Log In Sign up
The largest database of trusted experimental protocols

Nbp1 40256

Manufactured by Novus Biologicals
Visit Supplier
Sourced in United Kingdom

NBP1-40256 is a laboratory reagent produced by Novus Biologicals. It is intended for use in scientific research applications.

Automatically generated - may contain errors

Lab products found in correlation

Nb600 1335, by Novus Biologicals (2 mentions) β actin, by Merck Group (2 mentions) Immobilon, by Merck Group (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Nb400 144, by Novus Biologicals (1 mentions) Ubiquitin, by Proteintech (1 mentions) Ab3259, by Abcam (1 mentions) Ab11433, by Abcam (1 mentions) Ab30682, by Abcam (1 mentions) Ab140592, by Abcam (1 mentions) Amersham ecl prime, by GE Healthcare (1 mentions) Imagequant las 4000 mini, by GE Healthcare (1 mentions) Nb400 135, by Novus Biologicals (1 mentions) Subcellular protein fractionation kit for tissue, by Thermo Fisher Scientific (1 mentions) 4 to 12 bis tris gel, by Bio-Rad (1 mentions) Gp78 16675 1 ap, by Proteintech (1 mentions) Capturem ip co ip kit, by Takara Bio (1 mentions) Sc 2025, by Santa Cruz Biotechnology (1 mentions) Polyvinylidene difluoride membrane, by Bio-Rad (1 mentions) Mab374, by Merck Group (1 mentions)

6 protocols using nbp1 40256

1

Western Blot Analysis of ER Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue lysates were separated by SDS-PAGE and transferred to 0.45 μm polyvinylidene difluoride membranes (Immobilon, Millipore. Merck KGaA, Darmstadt, Germany). Blots were incubated with antibodies directed against CD36 (NB400-144), ATF6 (NBP1-40256), and CHOP (NB600-1335) purchased from Novus Biologicals (Bio-Techne LD-R&D Systems Europe Ltd., Abingdon, UK). Equal loading of protein in each lane was verified by β-actin (A5441, Sigma–Aldrich, Merck KGaA, Darmstadt, Germany).
Navas-Madroñal M., Castelblanco E., Camacho M., Consegal M., Ramirez-Morros A., Sarrias M.R., Perez P., Alonso N., Galán M, & Mauricio D. (2020). Role of the Scavenger Receptor CD36 in Accelerated Diabetic Atherosclerosis. International Journal of Molecular Sciences, 21(19), 7360.
+ Open protocol
+ Expand
2

Investigating Protein Homeostasis Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
Livers were homogenized and lysed in a solution containing 20 mM Tris (pH 7.5) 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, and 1 mM sodium orthovanadate. Subcellular fractions were obtained using Subcellular Protein Fractionation Kit for Tissues (Thermo Fisher Scientific). Immunoprecipitation was performed with each antibody using Capturem IP & Co-IP kit (Takara). Samples were separated by SDS-PAGE using 4 to 12% Bis-Tris gel (Bio-Rad) and transferred to a polyvinylidene difluoride membrane (Bio-Rad). Membranes were immunoblotted with each antibody. Amersham ECL prime (GE Healthcare Life Sciences) and ImageQuant LAS 4000mini (GE Healthcare Life Sciences) were used for the detection and quantification. Antibodies used in the present study are as follows: SREBP1 (ab3259; Abcam), VCP (ab11433; Abcam), S1P (ab140592; Abcam), SREBP2 (ab30682; Abcam), CHOP (2895; Cell Signaling Technology), BiP (3177; Cell Signaling Technology), β-actin (3700; Cell Signaling Technology), RHBDL4 (20869-1-AP; Proteintech), TBP (22006-1-AP; Proteintech), gp78 (16675-1-AP; Proteintech), HRD1 (13473-1-AP; Proteintech), Ubiquitin (10201-2-AP; Proteintech), carbohydrate-responsive element–binding protein (NB400-135; Novus Biologicals), ATF6 (NBP1-40256; Novus Biologicals), and normal mouse IgG (sc-2025; Santa Cruz Biotechnology).
Shibuya K., Ebihara K., Ebihara C., Sawayama N., Isoda M., Yamamuro D., Takahashi M., Nagashima S, & Ishibashi S. (2022). AAA-ATPase valosin-containing protein binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4. The Journal of Biological Chemistry, 298(6), 101936.
+ Open protocol
+ Expand
3

Antibody-Based Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used in this study were ATF6 (100 ng/mL, NBP1‐40256, Novus Biologicals), CHOP (C/EBP‐homologous protein) (1:10.000, 2895, Cell Signaling, Danvers, MA), GRP78 (70 ng/mL, 11587‐1‐AP, Proteintech, Rosemont, IL), IRE1α phosphorylated at Ser724 (200 ng/mL, MBS9610512, MyBioSource, San Diego, CA), IRE1α (40 ng/mL, sc‐390960, Santa Cruz Biotechnology, Dallas, TX), eIF2α phosphorylated at Ser51 (1:10.000, 3398, Cell Signaling), eIF2α (1:10.000, 2103, Cell Signaling), GAPDH (100 ng/mL, MAB374; Sigma‐Aldrich, St. Louis, MO), and β‐actin (6.1 ng/mL, 4970; Cell Signaling Technology).
Cicalese S., Torimoto K., Okuno K., Elliott K.J., Rizzo V., Hashimoto T, & Eguchi S. (2022). Endoplasmic Reticulum Chemical Chaperone 3‐Hydroxy‐2‐Naphthoic Acid Reduces Angiotensin II‐Induced Vascular Remodeling and Hypertension In Vivo and Protein Synthesis In Vitro. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 11(23), e028201.
+ Open protocol
+ Expand
4

Western Blot Analysis of EMC3, ATF6, and α-Tubulin

Check if the same lab product or an alternative is used in the 5 most similar protocols
Retinas were homogenized in radioimmunoprecipitation assay buffer (RIPA) lysis buffer. After ultrasound and adjustment of protein concentrations, equal amounts of protein were loaded into sodium dodecyl sulfate-polyacrylamide gels. Standard Western blotting procedures were performed. EMC3 antibody (mouse, 1:500; HPA042372; Sigma-Aldrich), cyclic AMP-dependent transcription factor ATF-6 alpha (ATF6) antibody (rabbit, 1:500; NBP1-40256; Novus Biologicals), and α-tubulin antibody (mouse, 1:1000; 660331-1-lg; ProteinTech Group, Rosemont, IL, USA) were incubated overnight at 4°C. The samples were then incubated with the IRDye Secondary Antibody (LI-COR; Biosciences, Lincoln, NE, USA), and signals were visualized using a near-infrared imaging system (Odyssey CLX, LI-COR; Biosciences) and quantified using the ImageJ software. The α-tubulin was used as a loading control. All the original Western blot bands were displayed (Supplementary Fig. S2).
Cao X., An J., Cao Y., Lv J., Wang J., Ding Y., Lin X, & Zhou X. (2021). EMC3 Is Essential for Retinal Organization and Neurogenesis During Mouse Retinal Development. Investigative Ophthalmology & Visual Science, 62(2), 31.
+ Open protocol
+ Expand
5

Studying ER Stress Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine recombinant insulin-like growth factor-1 (IGF-1) was purchased from PeproTech (Rocky Hill, NJ). Thapsigargin was obtained from Applichem (St Louis, MO), tunicamycin from Sigma (St Louis, MO) and brefeldin A from TOKU-E (Bellingham, WA). mTOR and PERK inhibitors were purchased from LC Labs (Woburn, MA) (rapamycin), Selleckchem (Houston, TX) (WYE125132, GSK2656157), Abcam (Cambridge, UK) (PP242) and from GlaskoSmith and Kline (Middlesex, UK) (GSK2126458). Antibodies against phospho-PERK (Thr980) (No.3179), PERK (No. 3192), phospho-eIF2α (Ser51) (No. 3597), eIF2α (No.2103), phospho-p70S6K1thr389 (No. 9234), p70S6K1 (No. 9202) and cleaved-caspase3 (No. 9664) were purchased from Cell Signaling Technology (Beverly, MA). Phospho-4E-BP1 (phosphorylation on Thr45; ab68187), and 4E-BP1 (ab2606) were from Abcam (Cambridge, UK). ATF6 clone 70B1413.1 were from Abcam (Cambridge, UK; ab11909) and Novus Biological (Littleton, CO; NBP1-40256). Antibodies against CHOP (SC-575), ATF4 (SC-200), GADD34 (SC-8327) and XBP1 (SC-7160) came from SantaCruz Biotechnology (Santa Cruz, CA) and BiP (610978) from BD Laboratories™ (Franklin Lakes, NJ). Antibody against α-tubulin was from Sigma-Aldrich (St. Louis, MO).
Freis P., Bollard J., Lebeau J., Massoma P., Fauvre J., Vercherat C., Walter T., Manié S., Roche C., Scoazec J.Y, & Ferraro-Peyret C. (2017). mTOR inhibitors activate PERK signaling and favor viability of gastrointestinal neuroendocrine cell lines. Oncotarget, 8(13), 20974-20987.
+ Open protocol
+ Expand
6

Western Blot Analysis of UPR Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue lysates were separated by SDS-PAGE and transferred to 0.45 μm polyvinylidene difluoride membranes (Immobilon, Millipore. Merck KGaA, Darmstadt, Germany). Blots were incubated with antibodies directed against ATF6 (NBP1-40256), XBP-1 (NBP1-75514), CHOP (NB600-1335), dynamin related protein 1 (DRP-1; NB110-55288) and p62 (NBP1-48320) purchased from Novus Biologicals (Bio-Techne LD-R&D Systems Europe Ltd, Abingdon, UK); rabbit polyclonal antibodies against Beclin 1 (PA1-16857) and LC3A/LC3B (PA1-16931)
were obtained from Pierce (ThermoFisher Scientific). Equal loading of protein in each lane was verified by β-actin (A5441, Sigma Aldrich).
Navas-Madroñal M., Rodriguez C., Kassan M., Fité J., Escudero J.R., Cañes L., Martínez-González J., Camacho M, & Galán M. (2019). Enhanced endoplasmic reticulum and mitochondrial stress in abdominal aortic aneurysm. Clinical science (London, England : 1979), 133(13).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!