Hippocampal apoptotic proteins were assayed using ELISA kits for Bax and Bcl-2 (BioVision, Inc., Milpitas, CA, USA; catalog numbers E4513 and CSB-E08854r, respectively). A colorimetric kit was used to measure caspase-3 activity (Sigma-Aldrich: CASP3C-1KT). Cytochrome c and brain-derived neurotrophic factor (BDNF) were determined using ELISA kits sourced from Abcam (catalog numbers: ab210575 and ab213899, respectively). All assays were performed according to the manufacturer’s instructions.
Casp3c 1kt
Manufactured by Merck Group
CASP3C-1KT is a laboratory product manufactured by Merck Group. It is a kit that contains materials and reagents used for detection and quantification of caspase-3, an enzyme involved in the process of apoptosis, or programmed cell death. The kit provides the necessary components to perform biochemical assays to measure caspase-3 activity. No further details on intended use or applications are provided.
Automatically generated - may contain errors
6 protocols using casp3c 1kt
1
Hippocampal Apoptotic Protein Assays
2
Cerebral Cortex Apoptosis Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cerebral cortex apoptotic proteins were assessed using ELISA kits for Bax and Bcl-2 (BioVision, Inc., Milpitas, CA, United States; catalog numbers E4513 and CSB-E08854r, respectively). A colourimetric kit was used to measure caspase-3 activity (Sigma-Aldrich: CASP3C-1KT). All assays were performed according to the manufacturer’s instructions.
3
Apoptotic Protein Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentration of apoptotic proteins including cytochrome c, Bax, and Bcl-2 was measured using enzyme-linked immunosorbent assay kits obtained from Cusabio (catalogue number CSB-EL006328RA), BioVision, Inc. (catalogue number E4513), and Cusabio (catalogue number CSB-E08854r) according to the suppliers' protocols, respectively, while caspase-3 activity is measured by using a calorimetric kit (Sigma-Aldrich, CASP3C-1KT) based on the hydrolysis of Ac-DEVD-pNA by caspase-3, resulting in the release of the p-nitroaniline (pNA) moiety. pNA is detected at 405 nm (εmM = 10.5).
4
Caspase 3 Activity Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Caspase 3 activity was analyzed in total homogenate using a colorimetric kit from Sigma Aldrich (CASP3C-1KT).
5
Apoptotic Protein Estimation in Hippocampus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The apoptotic proteins in the hippocampal tissue were estimated using enzyme-linked immunosorbent assay kits; Bax (BioVision, Inc., catalog number E4513) and Bcl-2 (BioVision, Inc., catalog number CSB-E08854r), whereas caspase 3 activity was measured by using a colorimetric kit (Sigma-Aldrich, CASP3C-1KT).
6
Caspase-3 Activity Assay in HTR-8/SVneo Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Based on the instructions, caspase-3 activity of HTR-8/SVneo cells was determined with a Caspase-3 assay kit (CASP3C-1KT, Sigma-Aldrich) after 48 h of transfection. The transfected cells were lysed with the lysis buffer from the kit, followed by centrifugation at 2000 g for 15 min. The supernatant of the lysed cells was collected and reacted with Ac-DEVE-pNA and reaction buffer. The optical density at 405 nm was determined as the caspase-3 activity.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!