Levels of inflammatory factors were measured by ELISA with Interleukin- 6 (IL-6) kit (MAN0017508, 88-7064) and tumor necrosis factor-α (TNF-α) kit (MAN0017423, 88-7324) purchased from Thermo Fisher Scientific (Massachusetts, America). Heart tissue was cut up, added to nine times the volume of PBS (pH = 7.4), transferred to a glass homogenizer and made into a 10% homogenate on ice, then further ground using ultrasonic fragmentation. The prepared tissue homogenate was centrifuged at 5,000 r for 5 min and the supernatant was taken as reserve. Dilute the antibody with carbonate-coated buffer to a protein content of 10 μg/ml. Antibodies, enzyme conjugates and chromogenic substrates were added according to the instructions of the kit, and the optical density value of each well was measured at 450 nm using a microplate reader after termination.
Tnf α kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The TNF-α kit is a laboratory assay designed to detect and quantify the presence of the tumor necrosis factor alpha (TNF-α) protein in biological samples. TNF-α is a key cytokine involved in inflammatory and immune responses. The kit uses a sandwich ELISA (Enzyme-Linked Immunosorbent Assay) format to measure TNF-α concentrations in the sample.
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Lab products found in correlation
2 protocols using tnf α kit
1
Quantification of Inflammatory Factors
2
Baicalin Anti-Inflammatory Compound Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Baicalin active pharmaceutical ingredients (API) (Xi'an Baikang Biotechnology Co., Ltd., Xi'an, China); Baicalin Control (China Academy of Food and Drug Administration, Beijing, China); Methanol (Shanghai McLean Biotechnology Co., Ltd., Shanghai, China); Polyvinyl Alcohol (Chengdu West Asia Chemical Co., Ltd., Chengdu, China); Ethyl Cellulose (Chengdu West Asia Chemical Co., Ltd., Chengdu, China); Methylene Chloride (Sinopharm Group Chemical Reagent Co. Ltd., Shanghai, China); phosphoric acid (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China); foetal bovine serum (Biological Industries, USA); sodium bicarbonate (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China); trichloromethane (Tianjin YD Chemical Reagent Co., Ltd., Tianjin, China); methanol (Tianjin YD Chemical Reagent Co. Ltd., Tianjin, China); ethanol (Tianjin Yongda Chemical Reagent Co., Ltd., Tianjin, China); isopropanol (Tianjin Yongda Chemical Reagent Co., Ltd., Tianjin, China); IL-1β kit (Thermo Fisher Scientific, USA); TNF-α kit (Thermo Fisher Scientific, USA); ELISA reagents (SINOPACIFIC Group Chemical Reagent Co. Ltd., Shanghai, China); ELISA kit (Thermo Fisher Scientific, USA); ultrapure water (Speed Instrument Technology Co., Ltd., Shanghai, China).
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