Z serum clot activator vacuette tube

For serum chemistries, whole blood was collected into Z Serum Clot Activator Greiner Vacuette tubes (Greiner Bio-One, Monroe, NC). Tubes were allowed to clot for 30–60 min and the serum separated in a centrifuge set at 1800 × g for 10 min at ambient temperature. The required volume of serum was removed for chemistry analysis using a General Chemistry 13 panel (Abaxis, Union City, CA) on a Piccolo Point-Of-Care Analyzer (Abaxis, Union City, CA). Serum was removed from the clot within 1 hour of centrifugation and was analyzed within 12 hours of collection.
For hematology, whole blood was collected into Greiner Vacuette blood tubes containing K3 EDTA as an anti-coagulant. Hematology was performed on the Hemavet 950 FS (Drew Scientific, Waterbury, CT) within 4 hours of collection. Following completion of the complete blood count analysis, 1.5 mL of whole blood was added to 1.5 mL of phosphate buffered saline (w/o calcium or magnesium) (PBS-/-; Life Technologies, Grand Island, NY) for PBMC isolation.

For serum chemistries, whole blood was collected into Z Serum Clot Activator Greiner Vacuette tubes (Greiner Bio-One, Monroe, NC). Tubes were gently inverted by hand 5–10 times and placed upright. Tubes were allowed to clot for 30–60 minutes and the serum separated in a centrifuge set at 1800 x g for 10 minutes at ambient temperature. The required volume of serum was removed for chemistry analysis using a Piccolo Point-Of-Care Analyzer (Abaxis, Union City, CA). Serum was removed from the clot within 1 hour of centrifugation and was analyzed within 40–70 minutes of collection.
For hematology, whole blood was collected into Greiner Vacuette blood tubes containing the anti-coagulant K3 EDTA. Tubes were gently inverted by hand 5–10 times to ensure adequate mixing. Hematology was performed on the ADVIA 120 (Siemens Healthcare, Malvern, PA) for the aerosol study and the Hemavet 950 FS (Drew Scientific, Waterbury, CT) for the IM study. Following completion of the CBC analysis, the remaining blood was centrifuged to separate the plasma in a centrifuge set at 2500 x g for 10 minutes at ambient temperature. Centrifugation occurred within 6 hours of the addition of the blood to the EDTA tube. A 200 μl volume of EDTA plasma was added to 600 μl of TRIzol LS (Life Technologies, Grand Island, NY) in preparation for qRT-PCR.

For serum chemistries, whole blood was collected into Z Serum Clot Activator Greiner Vacuette tubes (Greiner Bio-One, Monroe, NC). Tubes were allowed to clot for 30–60 min and the serum separated in a centrifuge set at 1800 × g for 10 min at ambient temperature. Serum was removed from the clot within 1 hour of centrifugation and was analyzed within 12 hours of collection. Chemistry analysis was performed on Piccolo Point-Of-Care Analyzers (Abaxis, Union City, CA) using a General Chemistry 13 panel (Abaxis, Union City, CA). Complete blood counts (i.e., hematology) were performed within 4 hours of collection on K3 EDTA whole blood using the Hemavet 950 FS instrument (Drew Scientific, Waterbury, CT).