Cells were treated as indicated in the appropriate figure legends and then lysed in CHAPS lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, 0.75% CHAPS, 5 μg/ml aprotinin, 5 μg/ml leupeptin, 1 mM sodium orthovanadate, 10 mM β-glycerol phosphate, 1 mM NaF, 1 mM DTT, 1 mM MgCl2, 1 μl/ml Pierce universal nuclease for cell lysis) for 20 min on ice and cleared by centrifugation. Equal amounts of lysates were separated by SDS-PAGE and proteins were detected by immunoblotting. Antibodies used were HA, RPA32, and RPA32 pS33 (1:1000, Bethyl, A300–246A). Quantification of immunoblots was performed with Image Lab 3.0 software.
Rpa32 ps33
Manufactured by Fortis Life Sciences
The RPA32 pS33 is a laboratory equipment product that serves a core function. Details on its intended use are not available.
Automatically generated - may contain errors
Lab products found in correlation
Dnapkcs ps2056, by Abcam (1 mentions)
Rpa32 pt21, by Abcam (1 mentions)
Rpa32 ps4 8, by Fortis Life Sciences (1 mentions)
Rfwd3, by Fortis Life Sciences (1 mentions)
Chk1 ps345, by Cell Signaling Technology (1 mentions)
Anti flag antibody, by Merck Group (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Dna pkcs, by Cell Signaling Technology (1 mentions)
Myc sc 40, by Santa Cruz Biotechnology (1 mentions)
2 protocols using rpa32 ps33
1
Immunoblotting Analysis of DNA Damage Response
2
DNA Damage Response Antibodies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were used in this study: PRP19 (ab27692, 1:400 IF, 1:1000 WB), ATM pS1981 (ab81292, 1:1000 WB), DNAPKcs pS2056 (ab18192, 1:1000 WB) and RPA32 pT21 (ab109394, 1:5000 WB) antibodies were from Abcam, CHK1 (SC-8404, 1:1000 WB), Myc (SC-40, 1:1000 WB) and HA (SC-7392, 1:1000 WB) antibodies were from Santa Cruz; RPA32 antibodies (MA1–26418, 1:500 IF, 1:1000 WB) were from Thermo, ATM (A300–299A, 1:1000 WB), RPA32 pS4/8 (A300–245A, 1:1000 WB), RPA32 pS33 (A300–246A, 1:5000 WB), RFWD3 (A301–397A, 1:250 IF, 1:1000 WB) antibodies were from Bethyl; ubiquitin antibodies were from Covance (MMS-257P, 1:1000 WB); CDC5L antibodies were from BD-Bioscience (612362, 1:1000 WB); anti-FLAG antibodies were from Sigma (F1804, 1:800 IF, 1:1000 WB); CHK1 pS345 (2348, 1:1000 WB), GAPDH (5174, 1:1000 WB), DNAPKcs (12311, 1:1000 WB) and tubulin (2144, 1:1000 WB) antibodies were from Cell Signaling.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!