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682 protocols using potassium hydroxide

1

Environmental Durability Testing of Materials

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1) High-temperature test: the sample was put into an oven (MTI, SS-00AB table dry oven) at a constant temperature of 80 °C and maintained for 1 wk; 2) Low-temperature test: the sample was put in a Dewar filled with liquid nitrogen for 1 wk. The tested sample was soaked in liquid nitrogen during the whole testing process; 3) Acid test: concentrated sulfuric acid (95 to 98%, Sigma-Aldrich) was diluted with deionized water. Its pH was adjusted to be around pH = 4 (tested by pH test strips, EMD Millipore). The sample was immersed in the solution continuously for 1 wk; 4) Alkali test: potassium hydroxide solution (pH = 10, tested by pH test strips, EMD Millipore) was prepared by potassium hydroxide (Sigma-Aldrich) and deionized water. The sample was immersed in the solution continuously for 1 wk. The MIR spectra of samples were measured, and photographs were taken before and after tests.
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2

Preparation of Ethanol, Phenol, and Glycogen Solutions

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A 95% ethanol solution (made with Analytical Grade ethanol of at least 99.8% purity (CarloErba Reagents, Val-de-Reuil, France) was used. A 5% phenol solution was created by mixing 50 g of phenol (Sigma Chemical Co., St. Louis, MO, USA) with solvent. A 30% potassium hydroxide and sodium sulfate solution was prepared by adding 180 g of potassium hydroxide (Sigma Chemical Co., St. Louis, MO, USA) as a solvent. A stock glycogen standard solution was made by dissolving 50 milligrams of cattle glycogen powder (Sigma Co., St. Louis, MO, USA) in 10 milliliters of solvent to reach a concentration of 5 milligrams per milliliter.
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3

Quantification of Phytosterol Compounds

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Reference standards with a purity assay greater than 95% were purchased from Sigma Aldrich (Sydney, Australia), including cholesterol, stigmasterol, stigmastanol, brassicasterol, lathosterol, lanosterol, 5b-cholestan-3aol, β-sitosterol, and campesterol (purity assay 65%). All reference standards were prepared in heptane as 500 mg/L stock solutions. An acid hydrolysis solution of 8 M hydrochloric acid diluted in ethanol was prepared before analysis. A saponification solution of 5 M potassium hydroxide (Sigma Aldrich), with the potassium hydroxide dissolved in water, was made up in ethanol to volume. N-O-bis-(trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane was obtained from Grace Davison (Columbia, MD); n-heptane, pyridine, and cyclohexane were obtained from Merck (Melbourne, Australia); and boiling chips were obtained from BDH (Sydney, Australia). Ultrapure, Type 1 water was used throughout the experiments and was obtained using a Millipore water purification system (Element A10; Millipore Sigma, Burlington, MA).
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4

Activated Carbon Preparation from Sawdust

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The sawdust used for the preparation of the activated carbon was obtained from the mushroom culture facility in the department. Phosphoric acid, potassium hydroxide, ferrous sulphate, sodium hydroxide, sodium dihydrogen orthophosphate, disodium hydrogen orthophosphate, graphite, potassium hexacyanoferrate(iii) trihydrate, potassium hexacyanoferrate(ii), hydrogen peroxide, hydrazine hydrate, potassium hydroxide, nitric acid and sulphuric acid were of analytical grade and were obtained from Merck (http://www.merckmillipore.com). The MP standard was purchased from Sigma-Aldrich. All the solutions were prepared in Milli-Q water (resistivity = 18.2 MΩ cm at 25 °C).
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5

Chitosan-Pectin Hydrogel for Lysozyme Encapsulation

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Chitosan with high molecular weight (310,000–375,000 Da) and a degree of a deacetylation >75% was supplied from Sigma Aldrich Co (St. Louis, MO, USA). Pectin from citrus peel with molecular weight 30,000–100,000 and a degree of esterification 63–66% (high methoxyl Pectin) was obtained from Fluka Chemie GmbH, Buchs, Switzerland. Potassium hydroxide was purchased from Merk (KGaAn Darmstadt, Germany). Lysozyme from chicken egg white was supplied from Sigma Aldrich Co (St. Louis, MO, USA). All the other reagents utilized were of analytical grade.
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6

Synthesis and Characterization of KAlSiO4 Catalyst

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Silicic acid, potassium hydroxide (≥ 85.0 %), and hydrochloric acid (37%) were purchased from Merk, and aluminum foil and MB were purchased from Fluka. X ray diffraction (XRD) pattern was recorded with an X ray diffractometer (SHIMADZUXD-DL) using Cu K α radiation (λ = 1.5418 Å) at 35.4 kV and 28 mA with a scanning speed of 2θ = 10° min -1 . Fourier transform infrared (FT IR) spectrum was recorded with an FT IR spectrometer (Tensor 27 Bruker) at room temperature in the range of 400-1300 cm -1 . Scanning electron microscopy (SEM; EM-3200, KYKY) was used to determine the crystallite size and morphology of the sample. Nitrogen adsorption-desorption isotherms were measured at 77 K using a Quantachrome NovaWin2 apparatus. For each set of experiments, the residual MB concentrations in the supernatant were analyzed using a T90+ -UV-vis spectrophotometer. The pH measurements were made with a pH meter, model 827 pH lab, Metrohm, Switzerland.
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7

Metabolite Extraction and Derivatization

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Chloroform, methanol, potassium hydroxide, sodium bicarbonate, sodium hydroxide were purchased from MERK (Damstadt, Germany). The internal standard 2,3,3,3-d4-alanine, the derivatization reagent methylChloroformate (MCF), and pyridine were obtained from Sigma-Aldrich (St. Louis, USA). Anhydrous sodium sulphate and were obtained from Fluka (Steinheim, Germany). Sulphuric acid was brought from ROMIL Pure Chemistry (Lameira, England). All chemicals used in this research were of analytical grade.
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8

Organic Solvent Extraction and Purification

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Optima Grade Methanol was from Fisher Scientific (Fair Lawn, NJ, USA). Chloroform, heptane, and isooctane were HPLC grade from Sigma-Aldrich (St. Louis, MO, USA). Water was from an in-house Millipore (Bedford, MA, USA) Milli-Q Water Purification System. Potassium hydroxide (Aldrich Chemical Company, Milwaukee, WI, USA) and potassium chloride (Fisher Scientific) were reagent grade.
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9

Thiol-Based Self-Assembled Monolayers

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Thiophenol (TP), 4-fluorothiophenol (FTP), mercaptobenzoic acid (MBA), ethanethiol (ET), hexadecanethiol (HDT), mercaptopropionic acid (MPA), 11-mercaptoundecanoic acid (MUA), 16-mercaptohexdecanoic acid (MHA), and 1,4-mercaptobenzoic acid (MBA) were purchased from Aldrich. Potassium ferricyanide (99+%), hexaamineruthenium(iii) chloride (98%), potassium chloride (99+%), hydrochloric acid (ACS reagent), sodium sulphide (Na2S, ACS reagent), and potassium hydroxide (semiconductor grade, 99.99%) were used as received from Aldrich. Water was purified with a Millipore Milli-Q system.
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10

Synthesis of Heterocyclic Compounds

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Cyanuric chloride (99%), p-hydroxybenzaldehyde (98%), sodium hydroxide (NaOH) (99%), cyclohexanone (99.8%), 4-nitrobenzaldehyde (98%), potassium hydroxide (KOH) (≥ 85%), sodium sulfide (Na2S) (≥ 98%), sodium bicarbonate (NaHCO3) (99%), methyl red (MR) (≥ 99%), chlorhydric acid (HCl) (37%), acetone (99%), ethanol (99%), ethyl acetate (99.5%), n-hexane (99%), methanol (99%), n-butanol (99.5%), o-dichlorobenzene (≥ 99%), acetic acid (100%), N,N-dimethylformamide (DMF) (≥ 99.8%), dichloromethane (≥ 99.5%), and tetrahydrofuran (THF) (≥ 99.8%) were prepared from Aldrich and Merck companies and used without further purification.
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