SPF grade GK rats (male, ~300 g, 8–10 weeks old) were purchased from the Laboratory Animal Center of Zhejiang Chinese Medical University. All animal experimental experiments were performed following the guidelines for Animal Care and Use Committee. Microbubble contrast agents were purchased from Braco (Italy). Creatine kinase, lactate dehydrogenase, and cardiac troponin I assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (China). CXCR4 rabbit anti-mouse monoclonal antibody was purchased from Abcam (USA). Trizol RNA extraction reagent, Real-timeScript™ reverse transcription kit, and SYBR oride was purchased from Sigma-Aldrich (USA). SonoVue injection kit was purchased from BRACCO INTERNATIONAL BV. (Spain).
Trizol rna extraction reagent
Manufactured by Merck Group
Sourced in United States
Trizol is a reagent used for the extraction and purification of RNA from biological samples. It is a mono-phasic solution containing phenol and guanidine isothiocyanate, which helps to maintain the integrity of RNA during the extraction process. Trizol can be used to isolate RNA from a variety of sources, including cells, tissues, and biological fluids.
Automatically generated - may contain errors
Lab products found in correlation
Real timescript reverse transcription kit, by Merck Group (2 mentions)
Sybr oride, by Merck Group (2 mentions)
Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions)
Agilent 2200, by Agilent Technologies (1 mentions)
Dna clean beads, by Beckman Coulter (1 mentions)
Superscript 3 first strand synthesis system for rt pcr, by Thermo Fisher Scientific (1 mentions)
Dnase 1 amplification grade, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Transgene (1 mentions)
Genomic dna extraction kit, by Transgene (1 mentions)
Plasmid extraction kit, by Transgene (1 mentions)
Reverse transcription kit, by Transgene (1 mentions)
T4 ligase, by Transgene (1 mentions)
Anti mouse pd l1 antibody, by BioXCell (1 mentions)
Sybr green rt pcr master mix, by Transgene (1 mentions)
Escherichia coli dh5α, by Transgene (1 mentions)
5 protocols using trizol rna extraction reagent
1
Rat Myocardial Infarction Protocol
2
RNA Extraction and Sequencing of AAA Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from AAA tissues (N = 3) and normal abdominal aortic tissues (N = 3) with a Trizol RNA extraction reagent (Sigma–Aldrich, Shanghai, China). The quantity and purity of RNA were detected by the NanoDrop spectrophotometer (Thermo Fisher Scientific Inc., Shanghai, China). The RNA was electrophoresed on 1.2% agarose gel to analyze the integrity. For RNA-seq library construction, 1 μg of total RNA was used. The RNA libraries were constructed using the Total RNA-seq (HMR) Library Prep Kit (Azyme Biotech Co., Ltd., Nanjing, China). In brief, ribosomal RNA was removed with rRNA probes and RNase H, RNA was fragmented to ∼300 bp by metal ion, reverse transcribed into cDNA, and connected with adapters. The fragments were sorted by DNA Clean Beads (Beckman, USA), and subjected to PCR amplification and purification and libraries were validated using Agilent 2200 (Agilent, Santa Clara, CA, USA). Illumina platform (Illumina, Inc., CA, USA) was used for RNA sequencing.
3
Apoptosis and ROS Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
APS (purity >90%) was obtained from Tibobiochemistry Company (Beijing, China). The cellular apoptosis and ROS measurement kits were brought from Shanghai YoungChan Company (Shanghai, China). Trizol RNA extraction reagent, Real-timeScript™ reverse transcription kit, and SYBR oride were purchased from Sigma-Aldrich (USA). The SonoVue injection kit was purchased from BRACCO INTERNATIONAL BV (Spain). The antibodies targeting PPAR-γ, NF-κB, p-AMPK, t-AMPK, Beclin1 and Parkin were all brought from Abcam company (Cambridge, UK). Anti-mouse HRP secondary antibodies were also obtained from Abcam company (Cambridge, UK).
4
RNA Extraction and cDNA Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apterous adults (50 mg) of M. viciae and N. ribisnigri were homogenised separately in 1 mL TRIzol RNA extraction reagent (Sigma-Aldrich, St. Louis, MO) and total RNAs isolated following the manufacturer’s protocol. The integrity and purity of the total RNA were determined by agarose gel electrophoresis and the concentration measured using a spectrophotometer (NanoDrop ND-1000). Before cDNA synthesis, the RNA samples were treated with amplification grade DNase I (Life Technologies, Carlsbad, CA), according to the manufacturer’s instructions. cDNA was prepared from 1 μg of total RNA by reverse transcription, using SuperScript® III First-Strand Synthesis System for RT-PCR (Life Technologies, Carlsbad, CA), following the manufacturer’s protocol.
5
Comprehensive Molecular Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DMEM medium, fetal bovine serum, T4 ligase, T7 endonuclease, Escherichia coli DH5α, plasmid extraction kit, genomic DNA extraction kit, reverse transcription kit, and SYBR® Green RT-PCR Master Mix were purchased from TransGen Biotech Co (Beijing). ACCUTASE™ cell digestion solution was purchased from eBioScience Inc. Western blot antibodies, STAT1 (14994), p-STAT1 (Tyr701, 7649P). STAT3 (9132), p-STAT3 (Tyr705, 9145), STAT5 (9363p), p-STAT5 (Y694, 4322). AURKA (14475), p-AURKA (Thr288, 3079), PD-L1 antibody (13684), p-AKT (Ser473, 4060), AKT (pan) (4691), p-ERK/ERK antibody (4370), GAPDH (5174), β-tubulin (2128) were purchased from Cell Signaling Technology, CD3 histochemical antibody (100219), CD8 histochemical antibody (104705) were purchased from Biolegend, and 4% paraformaldehyde were purchased from Wuhan Seville Biological LTD. DAB histochemical staining kit was purchased from Zhongshan Jinqiao Biological Technology LTD (Beijing). ECL luminescence color reagent was purchased from Healthcare. Trizol RNA extraction reagent was purchased from Sigma-Aldrich (T9424). Anti-mouse PD-L1 Antibody was purchased from BioXcell.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!