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5 610 protocols using spss version 26

1

Soil Biota Analysis via PCoA and Statistics

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The principal coordinates analysis (PCoA), based on the Bray–Curtis distances at the out level, was conducted using the online platform (http://www.magichand.online, accessed on 4 April 2021) with R base programmes [23 (link)]. ANOISM (analysis of similarities) tests were used to define the significance of the differences between the control and planted groups, and a P of less than 0.05 indicated significance differences. Significant differences in the basic soil properties and diversity indices between the planted and control group were checked using t-tests in SPSS version 26, and a P of less than 0.05 indicated significance differences. Moreover, Pearson analysis was conducted to assess the basic soil properties and relative abundances of the top 20 AMF OTUs in SPSS version 26, and a P of less than 0.05 indicated a significant correlation. The figures were generated using Origin 2019.
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2

Metabolomic Biomarkers for Neurodegenerative Diseases

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The normality of individual metabolite was tested with the Shapiro–Wilk test using SPSS (version 26). The normally distributed data was analyzed using one-way ANOVA and Student’s t-test and non-distributed data analyzed using the Kruskal-Wallis test and multivariate analysis (PCA analysis) was performed using SIMCA 17. A heatmap was generated, and potential biomarkers and paired biomarker ratios were identified using MetaboAnalyst 5.0 (Pang et al., 2021 (link)). The receiver operating characteristic (ROC) analysis and bar chart was performed using GraphPad Prism 9. Spearman’s rank correlation coefficient was performed using SPSS (version 26) to determine the correlations between each metabolite and HMR, p-tau181, Aβ40, Aβ42, Aβ40/42, GFAP and NfL in AD and DLB subjects.
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3

ESBL and CPE Carriage Epidemiology

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All data were checked for completeness, coded, and entered using Epi-data version 4.6 and exported to SPSS version 26 for statistical analysis. Then data were analyzed using SPSS version 26 to determine the independent variables and the fecal carriage rate of ESBL and CPE frequency analysis and cross tab were used. The Chi-square test was used with appropriate correction for the observation. To determine the associated factors Multivariable Logistic regression analyses were used. A variable with a p-value ≤ 0.2 in bivariate logistic regression was checked in multivariate analysis for a statistically significant association by controlling the possible confounders. Crude and adjusted odds ratios were used to quantify the strength of association between ESBL-PE and CPE carriage rate and risk factors. A variable with a p-value of less than 0.05 at a 95% confidence interval was considered statistically significant.
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4

Bioactive Compounds and Antioxidant Activity

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The results are reported as the mean ± standard deviation (SD). Statistical analyses between samples were performed using nonparametric tests followed Kruskal-Wallis tests (SPSS version 26, SPSS Inc. Armonk, NY, USA). A significant difference was set at p < 0.05. Correlation analysis was performed using SPSS version 26, SPSS Inc., Armonk, NY, USA. Principal component analysis (PCA) of bioactive components between sprouts and their antioxidant activities was performed using Python software version 3.10.5 (Python Software Foundation, Fredericksburg, VA, USA). The bioactive compounds were normalized before computing the PCA analysis. The PCA scores and loading variables were obtained from the PCA model. The PCA score plot was used to illustrate the correlation between bioactive compounds and its antioxidant. The loading plot indicated the correlation between the bioactive compounds and their antioxidant activity at 0.5 (moderate correlation) and 1.0 (strong correlation) as a black dashed circle and a red solid circle, respectively.
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5

Assessing Intestinal Permeability by Dual Sugar Test

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Data were entered and cleaned in Epi Data version 3.1, then transferred to SPSS version 26 for analysis. Urine analysis was performed by HPLC. A dual sugar concentration in the urine was used to calculate the lactulose-to-mannitol ratio. Hemoglobin analysis was performed using hem cue 301 digital photometer. A data summary was performed using descriptive statistics. For continuous variables, the mean and standard deviation (SD) were used. A bivariate and multivariable logistic regression model was used to estimate crude and adjusted odds ratios with 95% confidence intervals. Variables with a p-value less than 0.05 were considered statistically significant.
Data were entered and cleaned in Epi Data version 3.1, then transferred to SPSS version 26 for analysis. Urine analysis was performed by HPLC. A dual sugar concentration in the urine was used to calculate the lactulose-to-mannitol ratio. Hemoglobin analysis was performed using hem cue 301 digital photometer. A data summary was performed using descriptive statistics. For continuous variables, the mean and standard deviation (SD) were used. A bivariate and multivariable logistic regression model was used to estimate crude and adjusted odds ratios with 95% confidence intervals. Variables with a p-value less than 0.05 were considered statistically significant.
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6

Statistical Analysis of Rice Straw Cultivars

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MS-Excel (Microsoft Office version 2010) was used to statistically analyse the biochemical properties of cultivars of rice straw, including descriptive statistics. The same data set was used to create a heat diagram with SPSS version 26.0 and a dendrogram-clustering with an average linkage between groups of 13 rice straw cultivars produced from hierarchical cluster analysis with SPSS version 26.0 (IBM Corp., 2019, Armonk, NY, USA).
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7

Factors Influencing Severe Trauma Outcomes

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Data analysis was performed using IBM SPSS version 26 (IBM Corp., Armonk, New York, USA). We completed the Shapiro–Wilk test for continuous variables, and none of the continuous variables showed a Gaussian distribution. Continuous variables are presented as medians and interquartile ranges. Categorical variables are presented as frequencies (percentages). Continuous variables were compared using the Wilcoxon rank-sum test, and categorical variables were compared using Fisher's exact test. Continuous variables were compared using the Mann–Whitney U test, and categorical variables were compared using Fisher's exact test. Statistical significance was set at P < 0.05.
Univariate and multivariate logistic regression analyses were performed to assess risk factors for severe trauma. All significant variables in the univariate analysis were subjected to multivariate logistic regression analysis. All variables with a P value of less than 0.05 were included in a logistic regression analysis. To evaluate the performance of the multivariate logistic regression model, a receiver operating characteristic (ROC) curve was generated, and the area under the receiver operating characteristics curve (AUROC) was calculated. SPSS version 26.0 (SPSS Inc., Chicago, IL, USA) was used for the statistical analysis.
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8

Propensity Score Matching Analysis of Recovery Factors

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We used mean and SD for normally distributed variables, or median (interquartile) for the variables not normally distributed, to summarize the participants' demographics. Statistical analyses were performed using SPSS version 26.0. Comparisons were performed using the t-test or Mann–Whitney test for continuous variables and chi-square test for categorical variables, as appropriate. All tests were two-sided, and an α <0.05 was considered significant.
Propensity score matching (PSM) was performed using SPSS version 26.0 to ensure an even distribution of possible confounders between the two groups. A 1:1 matching range by using proximity matching was performed with a caliper width of 0.01. The underlying characteristics considered in the propensity-matching process were age, gender, hypertension, diabetes mellitus, hyperlipidemia, psychiatric disease, perinatal period, history of BP, and time from the onset to medical interventions. After matching patient characteristics, the Kaplan–Meier method was used to estimate survival curves. Cox proportional hazards models were used to estimate the hazard ratio (HR) of recovery and the corresponding 95% confidence interval (CI).
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9

Validating Instrument Content and Construct

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We conducted a cross-sectional study of instrument content and construct validity. It consisted of developing tests and devices, including both the design or adaptation and the analysis of their psychometric properties [65] .
As a method to test the validity of the content, we used a panel of experts. To analyse the metric properties of each item, basic descriptive coefficients (mean, dispersion, kurtosis and skewness) were employed, with SPSS version 26.0. Kolmogorov-Smirnov and Levene's tests were performed to confirm normality and homoskedasticity of the sample. The validity of the construction was carried out through exploratory factor analysis (EFA) with Factor Analysis version 10.10.01 [66] (link), to determine the goodness of the fit and the validity of the scale [67] (link)[68] (link)[69] [70] , and confirmatory factor analysis (CFA) with M-PLUS, to establish the validity and reliability of the fit of the model [71, 72] . The internal consistency of the instrument was calculated using Cronbach's alpha coefficient with SPSS version 26.0, and the Composite Reliability (CR).
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10

Evaluating Changes in Goals of Care After Consultation

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Descriptive analysis of patient characteristics included frequencies, means, and standard deviations. A Wilcoxon signed-rank test was used to test for significant changes in GoC levels after consultation, and all models were tested for proportionality using the test of parallel lines. Univariate ordinal logistic regressions were used to determine whether age, frailty stage, cognition, or COVID-19 status were associated with changes in care plans after consultation. All regression models were controlled for pre-intervention GoC level. All analyses were conducted using R version 4.1.0 and IBM SPSS version 26. (R Core Team, Vienna, Austria) and IBM SPSS version 26 (IBM Corporation, Armonk, NY, USA).
The Nova Scotia Health Research Ethics Board approved this research (REB# 26635).
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