Ubiquinone (oCoQ10) (≥98%), ubiquinol (rCoQ10) (≥97%), and DL-α-tocopherol (≥97%) were purchased from Carbosynth (Berkshire, England). Anhydrous iron(II) chloride (≥98%) and iron(III) chloride (≥98%), ascorbic acid (≥99%), β-carotene (≥97%), 1,4-dithiothreitol (DTT) (≥99%), ellagic acid (≥95%), sodium sulfide (≥99%), quercetin (≥98%), 85% orthophosphoric acid (H3PO4), and HPLC grade solvents: acetonitrile (ACN), methanol (MeOH), n-hexane and tetrahydrofuran (THF) were obtained from Sigma-Aldrich (Steinheim, Germany). Anhydrous ethanol (EtOH) was obtained from Merck (Darmstadt, Germany). Ultra-pure water was obtained through a Milli-Q water purification system A10 Advantage (Millipore Corporation, Bedford, MA, USA).
Ortho phosphoric acid h3po4
Manufactured by Merck Group
Sourced in Germany, United States, India
Ortho-phosphoric acid (H3PO4) is a colorless, odorless, and viscous liquid that is commonly used in various laboratory applications. It is a strong mineral acid with a chemical formula of H3PO4. Ortho-phosphoric acid is known for its ability to act as a pH regulator, a buffer, and a complexing agent in various laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Merck Group (4 mentions)
Milli q advantage a10 water purification system, by Merck Group (2 mentions)
Tetrahydrofuran thf, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Trichloroacetic acid, by Merck Group (2 mentions)
Hydrogen peroxide h2o2, by Merck Group (2 mentions)
Sulfuric acid, by Merck Group (2 mentions)
Trizol, by Thermo Fisher Scientific (2 mentions)
Sodium sulfide, by Merck Group (1 mentions)
Anhydrous ethanol etoh, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
β carotene, by Merck Group (1 mentions)
1 4 dithiothreitol dtt, by Merck Group (1 mentions)
Iron 3 chloride, by Merck Group (1 mentions)
Ellagic acid, by Merck Group (1 mentions)
N hydroxysuccinimide nhs, by Thermo Fisher Scientific (1 mentions)
Prestoblue cell viability reagent, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by RCI Labscan (1 mentions)
24 protocols using ortho phosphoric acid h3po4
1
Antioxidant Compounds Characterization
2
Asiatic Acid-Hyaluronate Hydrogel Synthesis
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Asiatic acid (AA) (purity 95%) was obtained from SEPPIC, Normandie, France. Sodium hyaluronate (HA) (MW = 47 kDa) was purchased from Dali company (Wuhan, Hubei, China), amine-terminated poly(N-isopropylacrylamide) (pNIPAM) (Mn = 5.5 kDa) from Sigma-Aldrich, St. Louis, MO, USA, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) from US Biological Life Sciences and N-hydroxysuccinimide (NHS) from Acros Organics, Morris Plains, NJ, USA. Reagents required to prepare the mobile phase such as acetonitrile and methanol were purchased from RCI Labscan, Bangkok, Thailand, and 85% orthophosphoric acid (H3PO4) from Sigma-Aldrich, St. Louis, MO, USA. All chemical reagents were of analytical grade and all solvents were of high-performance liquid chromatography (HPLC) grade. For the cell culture experiment, mouse L-929 fibroblasts were obtained from the Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) and penicillin-streptomycin (10,000 U/mL) were purchased from Gibco, Waltham, MA, USA. PrestoBlueTM cell viability reagent was purchased from Invitrogen Corporation, San Diego, CA, USA. For the animal study, rats, strain BrlHan:WIST@Jcl (GALAS), were bought from Nomura Siam International, Bangkok, Thailand.
3
Simultaneous HPLC-UV Analysis of Vitamins
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All FSVs (vitamin A-palmitate, D3, E-acetate, K1 and β-carotene), butylated hydroxytoluene (BHT), anhy-Raku{a et al.: Novel HPLC-UV Method for Simultaneous ... drous ferric chloride, n-hexane, 85% orthophosphoric acid (H 3 PO 4 ) and HPLC grade: acetonitrile,methanol and tetrahydrofuran (THF) were purchased from Sigma-Aldrich (Steinheim, Germany). Coenzyme Q10 (CoQ10) was obtained from Kaneka Corporation (Osaka, Japan). Ultra-pure water was obtained through a Milli-Q water purification system A10 Advantage (Millipore Corporation, Bedford, MA, USA).
4
Quantitative Analysis of Sulfatase Activity
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Sulfatase from Helix pomatia, sinigrin hydrate, l -ascorbic acid (AA), trichloroacetic acid (TCA), dl -dithiothreitol (DTT), N-ethylmaleimide (NEM), 2,2′-bipyridyl, iron (III) chloride (FeCl3), 3-O-CQA (3-O-caffeoylquinic acid), 5-O-CQA (5-O-caffeoylquinic acid), butyl-isothiocyanate, caffeic acid (CA), 1,2-benzenedithiol, ß-mercaptoethanol (HPLC grade), methylene chloride (HPLC grade), sephadex A-25, sephadex C-25, acetonitrile (HPLC grade), methanol (HPLC grade), sulforaphane, sodium acetate, potassium phosphate, and orthophosphoric acid (H3PO4) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Desulfoglucoraphanin was obtained from Santa Cruz Biotechnology (Dallas, TX, USA).
5
Nitric Oxide and Cytokine Evaluation
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Nitric oxide (NO) evaluation was indirectly determined by nitrite concentration by the Griess colorimetric method [43 (link)]. Thus, 50 μL of supernatant from each well was added to 50 μL of Griess reagent (1:1 mixture of a 1% solution of sulfanilamide (Sigma-Aldrich, St. Louis, MO, USA) in 2.5% orthophosphoric acid (H3PO4) (Sigma-Aldrich, St. Louis, MO, USA) and 0.1% solution of N-(1-naphthyl)-ethylenediamine dihydrochloride (Sigma-Aldrich, St. Louis, MO, USA) in a 96-well microplate (Jet Biofil, Guangzhou, China). After 10 min, the optical density (absorbance) was spectrophotometrically (Biochrom, Cambridge, UK) evaluated by ELISA reader with length waveform of 570 nm. Results were expressed in μM of sodium nitrate (NaNO2) (Sigma-Aldrich, St. Louis, MO, USA), based on a standard reference curve. Cytokines IL-1β (Cat. No. 3259705), TNF-α (Cat. No. 9008894), and IL-6 (Cat. No. 7333548) were quantified by a BD OptEIA™ enzyme-linked immunosorbent assay (ELISA) test (BD Biosciences, San Jose, CA, USA) following the manufacturer’s instructions.
6
Synthesis and Evaluation of Au/Gd Nanoparticles
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Graphite flakes (100 mesh size), protocatechuic acid (PA) (98%—170.12 g/mol), phosphate-buffered saline (PBS) potassium permanganate (KMnO4) (99%), sulphuric acid (H2SO4) (98%), hydrogen peroxide (H2O2) (35%) and ortho-phosphoric acid (H3PO4) (85%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hydrochloric acid (HCl) (37%) and diethyl ether (85%) were supplied by Friedemann Schmidt (Parkwood, WA, USA). Ethyl alcohol (99.7%) was obtained from Hayman. Tetrachloroauric (III) acid trihydrate (49% Au—393.83 g/mol) and gadolinium (III) nitrate hexahydrate (99.9%) were supplied by Acros Organics (Morris Plains, NJ, USA), while human liver hepatocellular carcinoma (HepG2) and normal fibroblast (3T3) cell lines were supplied from the American Tissue Culture Collection (ATCC) (Manassas, VA, USA). Deionized water (DI) was used throughout the experiment.
7
Synthesis and Characterization of Biomaterials
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All of the chemicals used
in the study were at their highest integrity.
Calcium hydroxide (Ca(OH)2) was purchased from Xilong Scientific
Co., Ltd., (China). Orthophosphoric acid (H3PO4) and sodium hydroxide (NaOH) were purchased from Sigma-Aldrich (China).
Trisodium citrate (Na3C6H5O7) was obtained from Hangzhou Dayangchem Co., Ltd. (China). Urea molecules
(CO(NH2)2) and other chemicals including zinc
chloride (ZnCl2), copper chloride (CuCl2), and
ferrous chloride (FeCl2) were purchased from Zouping Zhijin
New Material Technology Co., Ltd. (China) and used without any further
purification. During the experimental period, ultrapure water (Evoqua
Type-I, Germany; resistivity <18.2 MΩ) was used for all of
the preparations and measurements.
in the study were at their highest integrity.
Calcium hydroxide (Ca(OH)2) was purchased from Xilong Scientific
Co., Ltd., (China). Orthophosphoric acid (H3PO4) and sodium hydroxide (NaOH) were purchased from Sigma-Aldrich (China).
Trisodium citrate (Na3C6H5O7) was obtained from Hangzhou Dayangchem Co., Ltd. (China). Urea molecules
(CO(NH2)2) and other chemicals including zinc
chloride (ZnCl2), copper chloride (CuCl2), and
ferrous chloride (FeCl2) were purchased from Zouping Zhijin
New Material Technology Co., Ltd. (China) and used without any further
purification. During the experimental period, ultrapure water (Evoqua
Type-I, Germany; resistivity <18.2 MΩ) was used for all of
the preparations and measurements.
8
Hyperforin Quantification by HPLC
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Hyperforin (Figure 1 , 99% purity) was purchased from Alexis Corp. (Lausen, Switzerland). (2‐Hydroxypropyl)‐β‐cyclodextrin and ortho‐phosphoric acid (H3PO4) were obtained from Sigma (St. Louis, MO, USA). HPLC grade methanol and acetonitrile were purchased from Merck (Darmstadt, Germany), and pure water was obtained from a Milli‐Q water purification system (Millipore, Bedford, MA, USA).
9
Collagen-based Calcium Phosphate Biomaterials
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Materials. Type I collagen extracted from equine tendon was provided from OPOCRIN Spa (Corlo di Formigine, MO, Italy). Calcium hydroxide (Ca(OH)2, ≥ 96.0 pure) was acquired to Fluka. Orthophosphoric acid (H3PO4, ACS reagent, ≥ 85% in H2O) and Terbium (III) chloride anhydrous (TbCl3, powder, 99.9% trace metals basis), were purchased to Sigma-Aldrich. All solutions were prepared with ultrapure water (0.22 μS, 25 °C, Milli-Q, Millipore).
10
Quantification of Antiviral Compounds by HPLC
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HPLC grade methanol, acetonitrile and water, orthophosphoric acid (H3PO4), and monopotassium phosphate (KH2PO4) were purchased from Sigma-Aldrich (Saint-Louis, USA). In addition, chloroquine (CHLO), ritonavir (RIT), and indomethacin (IND) drugs were also purchased from Sigma-Aldrich (Saint-Louis, USA) with a purity ≥ 98%. Standard stock solutions of 1000 mg L−1 were prepared in methanol or HPLC grade water, depending on the compound's solubility. These solutions were stored at 4 °C. Then freshly prepared working solutions were obtained by diluting the prepared stock solutions. The chemical structures of all studied compounds are shown in Table 1 .
Molecular structure and main physicochemical properties of the studied antivirals
| Drug | Molecular structure | Brut formula | pKa |
|---|---|---|---|
| Chloroquine | C18H26ClN3 | 10.1 | |
| Indometacin | C19H16ClNO4 | 4.50 | |
| Ritonavir | C37H48N6O5S2 | 2.84 13.68 |
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