Mitoscreen jc 1

Manufactured by BD

Sourced in United States

BD MitoScreen (JC-1) is a laboratory instrument used for the detection and analysis of mitochondrial membrane potential. It utilizes the dye JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide) to assess mitochondrial function in cells.

Automatically generated - may contain errors

Lab products found in correlation

Fitc annexin 5 apoptosis detection kit 1, by BD (3 mentions) Facscalibur flow cytometer, by BD (1 mentions) Accuri c6, by BD (1 mentions) Annexin 5, by BD (1 mentions) Mitochondrial membrane potential detection kit, by Solarbio (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Xf cell mito stress test kit, by Agilent Technologies (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Facscalibur, by BD (1 mentions) Cytochrome c, by Cell Signaling Technology (1 mentions) Cellrox green, by Thermo Fisher Scientific (1 mentions) P h2ax, by Cell Signaling Technology (1 mentions) Fixation permeabilization solution kit, by BD (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Pe rabbit anti active caspase 3, by BD (1 mentions) Alexa fluor 700 mouse anti cleaved parp asp214, by BD (1 mentions) Cleaved caspase 8, by Cell Signaling Technology (1 mentions) Cell counting kit 8 (cck8), by Merck Group (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)

12 protocols using mitoscreen jc 1

Annexin V-PE/7-AAD Apoptosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

After the treatments, apoptosis was detected by flow cytometry using an annexin V- phycoerythrin (PE)/7-aminoactinomycin D (7-AAD) assay kit (Solarbio). The cells were collected and resuspended in binding buffer. The cells were stained with 5 μL of annexin V/PE at room temperature in the dark for 5 min. Then, 10 μL of 20 μg/mL 7-AAD and 400 μL of PBS were added and apoptotic cells were immediately detected using the BD FACSCalibur flow cytometer and analyzed using the CellQuest software.
The MMP was evaluated using MitoScreen (JC-1) (551302; BD Biosciences, Franklin Lakes, NJ, USA), which uses the membrane-permeable dye JC-1 to detect mitochondrial depolarization in cells. After the treatments, the cells were digested with trypsin and washed twice with binding buffer. Then, JC-1 was added for 30 min after which the cells were suspended in binding buffer. MMP levels were detected by using the FACSCalibur flow cytometer and analyzed using the CellQuest software. MMP is reflected by the proportion of JC-1 aggregates and monomers. The experiments were performed in triplicate.

Wang J., Chen M., Wang S., Chu X, & Ji H. (2022). Identification of Phytogenic Compounds with Antioxidant Action That Protect Porcine Intestinal Epithelial Cells from Hydrogen Peroxide Induced Oxidative Damage. Antioxidants, 11(11), 2134.

+ Open protocol

+ Expand

Apoptosis and Mitochondrial Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Apoptosis analyses were performed at the 48^th hour after anti-miR and prednisolone treatment by using Annexin V (BD Pharmingen, Cat. No: 556547) and MitoScreen JC-1 (BD Pharmingen, Cat. No: 551302) according to manufacturer's instructions. In addition, mitochondrial membrane potential changes were also detected by MitoScreen JC-1 assay. The results were evaluated by Bd Accuri C6 (BD Biosciences Pharmingen) flow cytometry.

Durmaz B., Bagca B.G., Cogulu O., Susluer S.Y., Alpay A., Aksoylar S, & Gunduz C. (2021). Antileukemic Effects of Anti-miR-146a, Anti-miR-155, Anti-miR-181a, and Prednisolone on Childhood Acute Lymphoblastic Leukemia. BioMed Research International, 2021, 3207328.

+ Open protocol

+ Expand

Mitochondrial Membrane Potential Analysis in COPD

Check if the same lab product or an alternative is used in the 5 most similar protocols

For recruited patients and healthy people, including 17 patients in the normal control group, 17 patients in the COPD group and 17 patients taking YQGB pills, peripheral blood lymphocytes were extracted using a lymph extraction kit (TBD, Tianjin, China). MitoScreen (JC-1) (BD) was used to detect the level of the mitochondrial membrane potential of peripheral blood lymphocytes in different groups. The extracted lymphocytes were stained using JC-1 and incubated for 15 min in the dark. Flow cytometry was performed after resuspension of the assay buffer.
The mitochondrial membrane potential of HBE cells was detected using a mitochondrial membrane potential detection kit (Solarbio, Beijing, China). The cell concentration was adjusted to 1×10⁶ cells/mL and then added to a 2 µm JC-1 probe, and the cells were incubated for 30 min at 37 ℃ in the dark. After being washed three times with phosphate buffered saline (PBS), the cells were stained with 4',6-diamidino-2-phenylindole (DAPI) to determine their nuclei, and then they were photographed and observed under a fluorescence microscope.

Meng T., Li F.S., Xu D., Jing J., Li Z., Maimaitiaili M, & Bao Y.J. (2024). Yiqigubiao pill treatment regulates Sirtuin 5 expression and mitochondrial function in chronic obstructive pulmonary disease. Journal of Thoracic Disease, 16(4), 2326-2340.

+ Open protocol

+ Expand

Apoptosis and Mitochondrial Stress Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum, and trypsin-EDTA purchased from Gibco^®; Life Technologies Inc. FITC Annexin V Apoptosis Detection Kit I obtained from BD Pharmingen, USA. MitoScreen (JC-1) and Via-probe (7-AAD staining) purchased from BD Bioscience, USA. XF Cell Mito Stress Test kit purchased from Seahorse Bioscience, USA.

Demeekul K., Suthammarak W, & Petchdee S. (2021). Bioactive Compounds from Germinated Brown Rice Protect Cardiomyocytes Against Simulated Ischemic/Reperfusion Injury by Ameliorating Mitochondrial Dysfunction. Drug Design, Development and Therapy, 15, 1055-1066.

+ Open protocol

+ Expand

Mitochondrial Membrane Potential in HPV16-Transduced Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Changes in mitochondrial membrane potential (ΔΨ) were determined in cultures of PHK transduced with a retroviral vector expressing HPV16 E6 and E7. Briefly, sub-confluent cultures of cells were treated with 2 nM of TNF as described above. Cells were then detached from the plates, centrifuged, and processed using the BD™ MitoScreen (JC-1) (BD Biosciences, San Jose, CA, USA) kit following the manufacturer’s instructions. Finally, at least 10,000 events per sample were acquired using a FACSCalibur (BD Biosciences, Carlsbad, CA, USA).

Cabeça T.K., de Mello Abreu A., Andrette R., de Souza Lino V., Morale M.G., Aguayo F., Termini L., Villa L.L., Lepique A.P, & Boccardo E. (2019). HPV-Mediated Resistance to TNF and TRAIL Is Characterized by Global Alterations in Apoptosis Regulatory Factors, Dysregulation of Death Receptors, and Induction of ROS/RNS. International Journal of Molecular Sciences, 20(1), 198.

+ Open protocol

+ Expand

Multiparametric Apoptosis Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

BTZ, Cell Counting Kit-8, and N-acetylcysteine (NAC) were purchased from Sigma Aldrich (St. Louis, MO, USA). z-VAD-FMK was purchased from Calbiochem (San Diego, CA, USA). Antibodies against Skp2, p21, p27, MTH1, CDK4, CDK6, caspase-9, Bcl-2, cleaved caspase-3, caspase-3, Bax, p53, Cytochrome c, PARP, Cleaved caspase-8, PH2AX were purchased from Cell Signaling Technology (Danvers, MA, USA). FITC Annexin V apoptosis detection kit I, Apo-Direct kit, Fixation/Permeabilization solution kit, BD MitoScreen (JC1), BV421 mouse anti-γH2AX (pS139), PE rabbit anti-active caspase-3, and Alexa Fluor 700 mouse anti-cleaved PARP (Asp214) antibodies were purchased from BD Biosciences (San Jose, USA). CellROXGreen was purchased from Invitrogen (Massachusetts, USA).

Prabhu K.S., Ahmad F., Kuttikrishnan S., Leo R., Ali T.A., Izadi M., Mateo J.M., Alam M., Ahmad A., Al-Shabeeb Akil A.S., Bhat A.A., Buddenkotte J., Pourkarimi E., Steinhoff M, & Uddin S. (2024). Bortezomib exerts its anti-cancer activity through the regulation of Skp2/p53 axis in non-melanoma skin cancer cells and C. elegans. Cell Death Discovery, 10, 225.

+ Open protocol

+ Expand

Mitochondrial Membrane Potential Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Changes in mitochondrial membrane potential were assessed by JC-1 staining as per manufacturer’s instruction (BD Biosciences). In brief, cells were cultured in a four-chamber slide and challenged with S. aureus for the indicated time points. Following challenge, cells were washed with PBS and incubated with BD MitoScreen (JC-1) (BD Biosciences) for 1 h. Cells were then visualized using an Eclipse 90i fluorescence microscope (Nikon). For quantitative analysis, cells were grown in a six-well plate and challenged with S. aureus RN6390 for indicated time periods. Cells were harvested by treating with TrypLE, washed and stained with JC-1 dye as indicated above. Cells were acquired by AccuriC6 flow cytometer with excitation at 488 nm and emission using 670 nm. At least 50 000 cells were analyzed in each treatment. The flow cytometric data were analyzed using AccuriC6 software.

Singh P.K, & Kumar A. (2016). Mitochondria mediates caspase-dependent and independent retinal cell death in Staphylococcus aureus endophthalmitis. Cell Death Discovery, 2, 16034-.

+ Open protocol

+ Expand

Investigating Autophagy Regulation in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Metformin (1,1-dimethylbiguanide hydrochloride), 3-methyladenine (3MA), chloroquine (CQ), and siRNA were purchased from Sigma Aldrich (St. Louis, MI, USA). Anti-actin antibody was purchased from Sigma; all other antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Modified Eagle’s medium (MEM), non-essential amino acids (NEAA), and trypsin/EDTA (0.25% trypsin, 1 mM EDTA) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Antibiotics/antimycotics (ABAM) were purchased from Gibco (Carlsbad, CA, USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo Laboratories (Tokyo, Japan). Caspase-Glo assay kits were purchased from Promega (Madison, WI, USA). FITC Annexin V apoptosis detection kit I, FITC BrdU Flow Kit, and BD MitoScreen (JC-1) were purchased from BD Pharmingen (San Diego, CA, USA). Acridine orange (AO) was purchased from Molecular Probes (Eugene, OR, USA). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, CA, USA).

Takahashi A., Kimura F., Yamanaka A., Takebayashi A., Kita N., Takahashi K, & Murakami T. (2014). Metformin impairs growth of endometrial cancer cells via cell cycle arrest and concomitant autophagy and apoptosis. Cancer Cell International, 14, 53.

+ Open protocol

+ Expand

Rinderol-Induced Mitochondrial Depolarization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Alterations of mitochondrial membrane potential (ΔΨm) in HeLa cells induced by exposure to rinderol were investigated using JC-1 cationic dye (BD™ MitoScreen (JC-1); BD Pharmingen, San Diego, CA, USA). Cells were cultured on 6-well plates after inoculation at a density of 2 × 10⁵ cells/well. After 24 h of incubation cells were treated with rinderol in concentrations and for time periods analogical to apoptosis detection assay. Staining was performed according to the manufacturer’s instructions and then examined by flow cytometry. Samples used as positive control were incubated with 10 µg/mL of carbonyl cyanide m-chlorophenyl hydrazone (CCCP; Sigma-Aldrich, St. Louis, MO, USA). Results were presented as the percent of cell population with depolarized mitochondrial membrane. For all assays, the number of gated events was set to 10,000. At least three independent trials were performed for each experiment.

Kawka M., Bubko I., Koronkiewicz M., Gruber-Bzura B., Graikou K., Chinou I., Jeziorek M., Pietrosiuk A, & Sykłowska-Baranek K. (2021). Polyurethane Foam Rafts Supported In Vitro Cultures of Rindera graeca Roots for Enhanced Production of Rinderol, Potent Proapoptotic Naphthoquinone Compound. International Journal of Molecular Sciences, 23(1), 56.

+ Open protocol

+ Expand

Multiparametric Assessment of Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were treated as indicated in the text. The media containing floating cells and the adherent cells, after trypsinization, were combined. Cells were centrifuged and the supernatant discarded. To assess cell viability, autophagic vacuole presence, and mitochondrial permeability on the resultant cell pellet, Fixable Viability Dye 780 (Thermo Fisher Scientific), CYTO-ID Autophagy detection kit 2.0 (Enzo Biochem, Farmingdale, NY), and BD MitoScreen (JC-1) (BD Biosciences, Franklin Lakes, NJ) dyes, were used respectively, according to the manufacturers’ instructions. Samples were measured by the Attune Nxt Flow Cytometer (Thermo Fisher Scientific). Forward and side scatter measurements were used to gate for singlets and exclude debris. Single-stain compensation controls were collected and gates were drawn accordingly.

Shaw J.J., Boyer T.L., Venner E., Beck P.J., Slamowitz T., Caste T., Hickman A., Raymond M.H., Costa-Pinheiro P., Jameson M.J., Fox T.E, & Kester M. (2020). Inhibition of Lysosomal Function Mitigates Protective Mitophagy and Augments Ceramide Nanoliposome-Induced Cell Death in Head and Neck Squamous Cell Carcinoma. Molecular cancer therapeutics, 19(12), 2621-2633.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!