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2 protocols using human ifnγ 285 if 100

1

Comprehensive Immune Marker Antibody Panel

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PBRM1 antibody (A301-591A) was from Bethyl Laboratories. Phospho-STAT1 antibody (clone ST1P-11A5; Tyr701; 33-3400), human CD3 antibody (clone F7.2.38; MA5-12577), human CD45RO antibody (clone UCHL1; MA5-11532), human CD4 antibody (clone 4B12; MS1528S0), and human CD8 antibody (clone C8/144B; MS457S0) were from ThermoFisher Scientific. Mouse CD3 antibody (D4V8L; 99940), mouse CD8 antibody(D4W22; 98941), mouse CD4 antibody (D7D2Z; 25229), human PD-L1 antibody (E1L3N; 13684), mouse PD-L1 antibody (D5V3B; 64988), mouse PD-1 antibody (D7D5W; 84651), PBRM1 antibody (D3F7O, 91894), JAK1 antibody (6G4, 3344), JAK2 antibody (D2E12, 3230), Phospho-JAK1 antibody (D7N4Z, Tyr1034/1035, 74129), Phospho-JAK2 antibody (C80C3, Tyr1007/1008, 3776), STAT1 antibody (D1K9Y, 14994), Phospho-STAT1 antibody (58D6, Tyr701, 9167), Phospho-STAT1 antibody (D3B7, Ser727, 8826), IRF1 antibody (D5E4, 8478), and BRG1 antibody (E9O6E; 52251) were from Cell Signaling Technology. IFNGR1 antibody (112802) was from BioLegend. IFNGR2 antibody (Cat No. GTX 64548) was from GeneTex. β-actin antibody (A1978) was from Sigma. Human IFNγ (285-IF-100) and mouse IFNγ (8234-MB-010) were from R&D Systems.
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2

Characterization of Sulfur Metabolism Proteins

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The following primary antibodies were used in this study: anti-SQOR (17256-1-AP) and anti-SLC7A11/xCT (26864-1-AP) from Proteintech, anti-caspase 1 (SC-515) from Santa Cruz Biotechnology, anti-NLRP3 (ag-20b-0014) and anti-ASC (AG-25B-0006) from AdipoGen, anti-IL-1β (5129-100) from Biovision, anti-GAPDH (ab9484) from Abcam. LPS from E. coli 055:B5 (L4005), PMA (P8139), dl-propargylglycine (p7888), aminooxyacetate (C13408) and sulfasalazine (S0883) were purchased from Sigma. Mouse IFN-γ (485-MI-100) and human IFN-γ (285-IF-100) were from R&D Systems. Poly (dA:dT) (tlrl-patn-1) and flagellin (tlrl-stfla) were from InvivoGen. EZ-Link Maleimide-PEG2-Biotin (21901BID), streptavidin-agarose resin (20361) and disuccinimidyl suberate (DSS) (21655) were from Thermo Scientific. The fluorescent probes WSP-5 and SSP4 were a kind gift of Prof. Ming Xian (Brown University). The MPST inhibitor I3-MT-3 was a kind gift of Prof. Kenjiro Hanaoka (Keio University, Tokyo, Japan). Tissue culture media and reagents were from Biological Industries (Beit Haemek, Israel).
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