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3 amino 9 ethylcarbazole substrate chromogen

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3-amino-9-ethylcarbazole substrate chromogen is a laboratory reagent used in various immunohistochemical and diagnostic applications. It functions as a chromogenic substrate, producing a colored reaction when exposed to certain enzymatic activities. This product enables the visualization of target analytes in biological samples.

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Lab products found in correlation

Biotin labeled secondary immunoglobulin, by Agilent Technologies (4 mentions) 3 3 diaminobenzidine tetrahydrochloride, by Merck Group (3 mentions) Cox 2, by Cell Signaling Technology (2 mentions) Ab7970, by Abcam (2 mentions) S100a9 nb110 89726, by Novus Biologicals (1 mentions) Ab109617, by Abcam (1 mentions) 3 3 diaminobenzidine tetrahydrochloride dab, by Merck Group (1 mentions)

Close spelling variants of this product

3-amino-9-ethylcarbazole (22 citations) 3-amino-9-ethylcarbazole plus substrate-chromogen (3 citations) 3-amino-9-ethylcarbazole substrate (2 citations) 3-amino-9-ethylcarbazole AEC+/substrate chromogen (2 citations)

9 protocols using 3 amino 9 ethylcarbazole substrate chromogen

1

Immunohistochemical Staining of SGO2 in Tissue Microarrays

Cited 3 times
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IHC staining was conducted with commercially available tissue microarrays (BS17015a and NGL961; Biomax, Rochester, NY, USA) according to previous protocol23 (link), 24 (link). The tissue microarrays were incubated with a polyclonal rabbit anti-human SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm. Sweden) which was diluted in phosphate buffered saline (PBS) at a ratio of 1:20 for 1 h at room temperature, washed 3 times (each for 5 min in PBS), incubated with biotin-labeled secondary immunoglobulin (1:100, DAKO, Glostrup, Denmark) for 1 h at room temperature, washed 3 times, and treated with 3-amino-9-ethylcarbazole substrate chromogen (DAKO) at room temperature to visualize peroxidase activity16 (link). Labeling index was scored accordance with multiplying quantity by intensity. The quantity was defined as Negative: 0, < 25%: 1, 25–75%: 2, and > 75%: 3 The intensity was defined as Negative: 0, Weak: 1, Moderate:2, and Strong: 325 (link).
Kao Y., Tsai W.C., Chen S.H., Hsu S.Y., Huang L.C., Chang C.J., Huang S.M, & Hueng D.Y. (2021). Shugoshin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas. Scientific Reports, 11, 18541.
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2

Immunohistochemical Protein Localization

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IHC was performed (10 (link)) using primary antibodies for PCNA (clone PC-10; Ab-1; Thermo Scientific), KRT14 (NCL-LL002; Novocastra), COX-2 (12282; Cell Signaling), S100A8 (T-1032; BMA), S100A9 (NB110-89726; Novus Biologicals), NF-κB p65 (ab7970; Abcam), STK40 (orb101780; Biorbyt), EGLN3 (orb443107; Biorbyt), and MBOAT2 (orb185503; Biorbyt). Protein was localized by incubation with the 3-amino-9-ethylcarbazole substrate chromogen (Dako) or 3,3′-diaminobenzidine tetrahydrochloride (Sigma-Aldrich).
Fong L.Y., Taccioli C., Palamarchuk A., Tagliazucchi G.M., Jing R., Smalley K.J., Fan S., Altemus J., Fiehn O., Huebner K., Farber J.L, & Croce C.M. (2020). Abrogation of esophageal carcinoma development in miR-31 knockout rats. Proceedings of the National Academy of Sciences of the United States of America, 117(11), 6075-6085.
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3

Immunohistochemical Staining of DDX3X

Cited 2 times
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IHC staining of tissue microarray (BS17016; Biomax, Rochester, NY, USA) was conducted according to previous protocol [26 (link),29 (link)], incubated with a polyclonal rabbit anti-human DDX3X antibody (N3C2) (1:250 diluted in phosphate buffered saline (PBS); GeneTex) for 1 h at room temperature, washed 3 times (each for 5 min in PBS), incubated with biotin-labeled secondary immunoglobulin (1:100, DAKO, Glostrup, Denmark) for 1 h at room temperature, washed 3 times, and treated with 3-amino-9-ethylcarbazole substrate chromogen (DAKO) at room temperature to visualize peroxidase activity [19 (link)]. Sections of breast cancer tissue (known to stain positive for DDX3X) were used as positive control [12 (link)].
Hueng D.Y., Tsai W.C., Chiou H.Y., Feng S.W., Lin C., Li Y.F., Huang L.C, & Lin M.H. (2015). DDX3X Biomarker Correlates with Poor Survival in Human Gliomas. International Journal of Molecular Sciences, 16(7), 15578-15591.
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4

IHC analysis of hexokinase 2 and PCNA

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FFPE tissues were deparaffinized, and rehydrated in graded alcohols. IHC was carried out as previously described [20 (link), 27 (link)], using anti-hexokinase 2 antibody [3D3] (#NBP1-51643, mouse monoclonal, Novus Biologicals, Littleton, CO, USA) and PCNA antibody (clone PC-10, Ab-1, Thermo Scientific, Waltham, MA, USA), after citrate-based antigen retrieval. Protein was localized by incubation with 3-amino-9-ethylcarbazole substrate-chromogen (Dako, Carpinteria, CA, USA) or 3,3’-diaminobenzidine tetrahydrochloride (Sigma-Aldrich, St Louis, MO, USA).
Fong L.Y., Jing R., Smalley K.J., Taccioli C., Fahrmann J., Barupal D.K., Alder H., Farber J.L., Fiehn O, & Croce C.M. (2017). Integration of metabolomics, transcriptomics, and microRNA expression profiling reveals a miR-143-HK2-glucose network underlying zinc-deficiency-associated esophageal neoplasia. Oncotarget, 8(47), 81910-81925.
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5

Immunohistochemical Analysis of FFPE Tissue

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Formalin-fixed, paraffin-embedded (FFPE) tissues were deparaffinized, and rehydrated in graded alcohols. IHC was carried out as previously described [37 (link), 38 (link), 60 ] using primary antibodies for PCNA (clone PC-10, Ab-1, Thermo Scientific, Waltham, MA, USA), KRT14 (NCL-LL002, Novocastra, Buffalo Grove, IL, USA), COX-2 (#12282, Cell Signaling, Danvers, MA, USA), S100A8 (T-1032, BMA, Augst, Switzerland), PDCD4 (LS-B1388, Lifespan Biosciences, Seattle, WA), STK40 (orb101780, Biorbyt, Cambridge, United Kingdom), FBXW7 (ab109617, Abcam, Cambridge, MA, USA), and PTEN (#9188, Cell Signaling) after citrate-based antigen retrieval. Protein was localized by incubation with 3-amino-9-ethylcarbazole substrate-chromogen (Dako, Carpinteria, CA, USA) or 3,3′-diaminobenzidine tetrahydrochloride (Sigma-Aldrich, St Louis, MO, USA).
Fong L.Y., Taccioli C., Jing R., Smalley K.J., Alder H., Jiang Y., Fadda P., Farber J.L, & Croce C.M. (2016). MicroRNA dysregulation and esophageal cancer development depend on the extent of zinc dietary deficiency. Oncotarget, 7(10), 10723-10738.
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6

Immunohistochemistry Analysis of FFPE Tissue

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IHC on FFPE sections was performed as previously described (36 (link)). Tissue sections were incubated with primary antibodies for mouse monoclonal PCNA (dilution 1:300, clone PC-10, Ab-1; Thermo Scientific), rabbit polyclonal NF-κΒ p65 (dilution 1:500, ab7970; Abcam), rabbit polyclonal COX-2 (dilution 1:300, NB1-689; Novus Biologicals), and rabbit polyclonal SLC39A1 (ZIP1) (dilution 1:2,000, NBP1-76498; Novus Biologicals), followed by incubation with appropriate biotinylated secondary antibodies and streptavidin HRP. Protein was localized by incubation with 3-amino-9-ethylcarbazole substrate-chromogen (Dako) or 3,3′-diaminobenzidine tetrahydrochloride (DAB) (Sigma-Aldrich).
Fong L.Y., Jing R., Smalley K.J., Wang Z.X., Taccioli C., Fan S., Chen H., Alder H., Huebner K., Farber J.L., Fiehn O, & Croce C.M. (2018). Human-like hyperplastic prostate with low ZIP1 induced solely by Zn deficiency in rats. Proceedings of the National Academy of Sciences of the United States of America, 115(47), E11091-E11100.
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7

IHC Staining of TELO2 in Bladder Cancer

Cited 2 times
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IHC staining of commercially available tissue microarray (BS17016; Biomax, Rochester, NY, USA) was performed according to previous protocol [29 (link)–31 (link)], incubated with a polyclonal rabbit anti-human TELO2 antibody (Cat. No. ab122722, Abcam, Cambridge, UK) (1:100 diluted in phosphate buffered saline (PBS) for 1 h at room temperature, washed 3 times (each for 5 min in PBS), incubated with biotin-labeled secondary immunoglobulin (1:100, DAKO, Glostrup, Denmark) for 1 h at room temperature, washed 3 times, and treated with 3-amino-9-ethylcarbazole substrate chromogen (DAKO) at room temperature to visualize peroxidase activity [32 (link)]. Sections of human urinary bladder cancer tissue (known to stain positive for TELO2) were used as positive control according to the datasheet of TELO2 antibody (ab122722).
Feng S.W., Chen Y., Tsai W.C., Chiou H.Y., Wu S.T., Huang L.C., Lin C., Hsieh C.C., Yang Y.J, & Hueng D.Y. (2016). Overexpression of TELO2 decreases survival in human high-grade gliomas. Oncotarget, 7(29), 46056-46066.
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8

Immunohistochemical Analysis of PSMB8 in Glioma

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A glioma tissue microarray (GL807a; Biomax) was incubated with a rabbit anti–human PSMB8 monoclonal antibody (Abcam) at room temperature. Information on the antibodies is presented in Table S1. After 16 hours of incubation, the samples were incubated with biotin‐labeled secondary immunoglobulin and treated with 3‐amino‐9‐ethylcarbazole substrate chromogen (DAKO, Glostrup, Denmark) to visualize peroxidase activity.
Chang H., Cheng Y., Tsai W, & Chen Y. (2020). PSMB8 inhibition decreases tumor angiogenesis in glioblastoma through vascular endothelial growth factor A reduction. Cancer Science, 111(11), 4142-4153.
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9

PSMB4 Protein Immunohistochemistry

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A tissue microarray (GL807a; Biomax, Rochester, NY, USA) was incubated with a monoclonal rabbit anti-human PSMB4 antibody (Cat. No. ab137067, Abcam, Cambridge, UK) at room temperature, incubated with biotin-labeled secondary immunoglobulin (1:100, DAKO, Glostrup, Denmark) at room temperature, and treated with 3-amino-9-ethylcarbazole substrate chromogen (DAKO) at room temperature to visualize peroxidase activity.
Cheng Y.C., Tsai W.C., Sung Y.C., Chang H.H, & Chen Y. (2018). Interference with PSMB4 Expression Exerts an Anti-Tumor Effect by Decreasing the Invasion and Proliferation of Human Glioblastoma Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 45(2).
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