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Anti egr 1

Manufactured by Santa Cruz Biotechnology
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Sourced in United Kingdom, United States

Anti-Egr-1 is an antibody product offered by Santa Cruz Biotechnology. It is designed to detect the Early Growth Response 1 (Egr-1) protein. Egr-1 is a transcription factor that plays a role in cellular growth and differentiation. The Anti-Egr-1 antibody can be used in various research applications to study the expression and function of Egr-1 in biological systems.

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Lab products found in correlation

Anti β actin, by Santa Cruz Biotechnology (2 mentions) Ez chip chromatin immunoprecipitation kit, by Merck Group (1 mentions) Alexa 647, by Jackson ImmunoResearch (1 mentions) Anti c fos, by Santa Cruz Biotechnology (1 mentions) Camkii, by Merck Group (1 mentions) Vectastain abc kit, by Vector Laboratories (1 mentions) Alexa 488, by Jackson ImmunoResearch (1 mentions) Anti creb, by Cell Signaling Technology (1 mentions) Cell counting kit 8 (cck8), by Keygen Biotech (1 mentions) Anti γ actin, by Abcam (1 mentions) Anti α tubulin, by Cell Signaling Technology (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Irdye secondary antibody, by LI COR (1 mentions) Anti hras, by Santa Cruz Biotechnology (1 mentions) Ab53281, by Abcam (1 mentions) Sc 13943, by Santa Cruz Biotechnology (1 mentions) Wbkls0100, by Merck Group (1 mentions) Anti bdnf, by Santa Cruz Biotechnology (1 mentions) Anti trkb, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Novus Biologicals (1 mentions)

Spelling variants of this product

Egr-1 (32 citations) Rabbit anti-Egr-1 (2 citations)

17 protocols using anti egr 1

1

ChIP-Seq Protocol for p53 and Egr-1

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Chromatin immunoprecipitation was carried out by using the EZ-ChIP Chromatin Immunoprecipitation Kit (Millipore) according to the manufacturer’s protocol. Briefly, cells were fixed with 1% formaldehyde at room temperature for 10 min to cross-link proteins to DNA. Then, the cross-linked chromatin was sonicated into 200 to 1000 bp fragments and immunoprecipitated using anti-p53 (Abcam, ab1101), anti-Egr-1 (Santa Cruz, sc-189) or IgG control antibodies. The samples were extensively washed after the cross-links were reversed and DNA was purified. The primers used for PCR amplification of specific promoter regions were listed in Additional file 4: Table S4.
Wang W., Xiong Y., Ding X., Wang L., Zhao Y., Fei Y., Zhu Y., Shen X., Tan C, & Liang Z. (2019). Cathepsin L activated by mutant p53 and Egr-1 promotes ionizing radiation-induced EMT in human NSCLC. Journal of Experimental & Clinical Cancer Research : CR, 38, 61.
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2

Immunohistochemical Analysis of c-Fos and Egr-1 Expression

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One hour after the SAT, mice were trans-cardially perfused with 4% paraformaldehyde (Sigma Aldrich, St. Louis, MO). The brains were post-fixed for 24 h and cryoprotected in a 30% sucrose solution. Coronal cryosections (40 µm) were incubated with a rabbit polyclonal anti-c- Fos (1:2000, Santa Cruz Biotechnology, Santa Cruz, CA; Figure 1A) or anti-Egr-1 (1:500, Santa Cruz Biotechnology, Santa Cruz, CA; Figure 4F) antibody for 48 hours at 4°C and then with secondary biotinylated anti-Rabbit IgG (1:200, Jackson Immunoresearch, West Grove, PA) antibody. The c-Fos and Egr-1 immunoreactivity were visualized using standard ABC method (Vectastain ABC kit, Vector) and diaminobenzidine (DAB)-nickel solution (Sigma Aldrich, St. Louis, MO). For double immunofluorescence experiments, cryosections were incubated with a mixture of rabbit polyclonal anti-c-Fos antibody (1:500, EMD Millipore, Billerica, MA) and either a mouse anti-calcium calmodulin kinase II (CaMKII, 1:250; EMD Millipore) or guinea pig anti-gamma-aminobutyric acid antibody (GABA, 1:200; EMD Millipore). A mixture of fluorescent secondary anti-rabbit and anti-mouse or anti-guinea pig antibodies (Alexa 488 and Alexa 546 or Alexa 647, 1:200; Jackson Immunoresearch) were used to visualize staining.
Ferri S.L., Kreibich A.S., Torre M., Piccoli C.T., Dow H., Pallathra A.A., Li H., Bilker W.B., Gur R.C., Abel T, & Brodkin E.S. (2016). Activation of Basolateral Amygdala in Juvenile C57BL/6J Mice During Social Approach Behavior. Neuroscience, 335, 184-194.
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3

NSCLC Tissue Collection and Analysis

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Paclitaxel and cisplatin were purchased from Suzhou Kowloon Hospital (Suzhou, China); Cell Counting Kit-8 were purchased from Keygen Biotech (Nanjing, China); The antibodies to TGF-β, smad3, p-smad2, p-smad3 and GAPDH were purchased from RUIYING Technology (Suzhou, China); anti-Egr-1 was obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA); anti-CREB was obtained from Cell Signaling Technology (Danvers, MA, USA); anti-CTSL was purchased from Abcam (Abcam, USA). All of the cell culture media and other reagents were from Invitrogen.
Human NSCLC tissue samples (n = 53) were collected from surgically resected specimens form patients in the Affiliated Hospital of Jiangsu University, Zhenjiang, China with written informed consent of patients.
Zhao Y., Shen X., Zhu Y., Wang A., Xiong Y., Wang L., Fei Y., Wang Y., Wang W., Lin F, & Liang Z. (2019). Cathepsin L-mediated resistance of paclitaxel and cisplatin is mediated by distinct regulatory mechanisms. Journal of Experimental & Clinical Cancer Research : CR, 38, 333.
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4

Immunoblot Analysis of Neuronal Proteins

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Protein concentrations were determined, and a total of 10 μg of protein was loaded onto 10% tris/SDS-polyacrylamide gels for electrophoresis separation and then transferred to nitrocellulose membranes, blocked with Rockland Blocking Buffer (VWR International), and incubated overnight at 4 °C with primary antibodies diluted in Rockland Blocking Buffer: anti-INO80 (1:1000; Bethyl Laboratories Inc., A303-371A), anti-TRIM3 (1:100; Santa Cruz Biotechnology, sc-136363), anti-EGR1 (1:100; Santa Cruz Biotechnology, sc-515830), anti–γ-actin (1:1000; Abcam, ab123034), anti–pan-SHANK [1:500; University California (UC) Davis/National Institutes of Health (NIH) NeuroMab Facility, 73–089], anti-GKAP/pan-SAPAP (1:500; UC Davis/NIH NeuroMab Facility, 73–156), and anti–α-tubulin (1:10,000; Cell Signaling Technology, 3837). After washing with tris-buffered saline (TBS) containing 0.1% Tween 20 (TBS-T), membranes were incubated with IRDye secondary antibodies (1:5000; LI-COR) in Rockland Blocking Buffer for 1 hour at room temperature. The blots were imaged using the Odyssey Infrared Imaging System (LI-COR) and quantified by densitometry using ImageJ (NIH). α-Tubulin was used as a protein loading control.
Werner C.T., Mitra S., Martin J.A., Stewart A.F., Lepack A.E., Ramakrishnan A., Gobira P.H., Wang Z.J., Neve R.L., Gancarz A.M., Shen L., Maze I, & Dietz D.M. (2019). Ubiquitin-proteasomal regulation of chromatin remodeler INO80 in the nucleus accumbens mediates persistent cocaine craving. Science Advances, 5(10), eaay0351.
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5

Immunohistochemical Analysis of Key Regulators

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Tissues were fixed with 4% paraformaldehyde, dehydrated, embedded in paraffin and sectioned at 4μm. Sections were immunohistochemically stained using mouse anti-human monoclonal anti-PKM2, anti-H-Ras, anti-EGR1, anti-HP1α, (Santa Cruz, Biotech). As the secondary antibody, anti-mouse IgG (Horseradish peroxidase linked whole antibody from sheep, GE Healthcare Limited) was used at 100×dilution. Staining was performed using 3, 3-diaminobenzidine (DAB) substrate kit for peroxidase according to the manufacturer's instructions (Vector Laboratories Inc) and counterstained with hematoxylin.
Li H., Li J., Jia S., Wu M., An J., Zheng Q., Zhang W, & Lu D. (2015). miR675 upregulates long noncoding RNA H19 through activating EGR1 in human liver cancer. Oncotarget, 6(31), 31958-31984.
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6

Isolation and Analysis of PBMCs

Cited 1 time
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Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood for qRT-PCR analysis as previously described (33 (link)); primers designed in this study are listed in Table S4. The proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and incubated with a specific antibody (anti-ADAM10, diluted 1:500, ab53281 [Abcam, Cambridge, UK], anti-EGR1, diluted 1:800, sc-13943 [Santa Cruz Biotechnology], and anti-β-actin, diluted 1:2,000 [Santa Cruz Biotechnology]) at 4°C overnight, followed by horseradish peroxidase-conjugated goat anti-rat IgG as the secondary antibody. A chemiluminescence detection kit (catalog no. WBKLS0100; Millipore, USA) was used to visualize immunoreactive bands.
Chen F., Wang Y., Zhang W., Cai Y., Zhao T., Mai H., Tao S., Wei W., Li J., Chen X., Li X., Tang P., Fan W., Yang J., Ou M., Lu F., Lai Z., Chen H., Zou T., Sun F., Shao Y, & Cui L. (2019). A Functional Polymorphism-Mediated Disruption of EGR1/ADAM10 Pathway Confers the Risk of Sepsis Progression. mBio, 10(4), e01663-19.
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7

Immunohistochemical Analysis of Neurotrophin Signaling

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All immunohistochemical experiments were performed on paraffin-embedded serial slides. Briefly, sample slides were deparaffinized and rehydrated and submerged into the EDTA antigenic retrieval buffer (pH 8.0) and microwaved for antigenic retrieval. After being blocked by normal goat serum for 30 minutes, the slides were incubated with the primary antibody (anti-TrkB, anti-BDNF, anti-NT-4/5, and anti-Egr-1; all from Santa Cruz Biotechnology Inc. [Dallas, TX, USA] 1:100 diluted in ChemMate antibody diluent, Dako Denmark A/S [Glostrup, Denmark]) for 18 hours. Subsequently, slides were washed with Tris-buffered saline (pH 7.4–7.6) and incubated for 45 minutes with the corresponding secondary antibodies conjugated with alkaline phosphatase (Rockland, Gilbertsville, PA, USA) at 1:250 dilution. Negative controls were prepared with the omission of the primary antibody.
Liu H., Yin T., Yan W., Si R., Wang B., Chen M., Li F., Wang Q, & Tao L. (2017). Dysregulation of microRNA-214 and PTEN contributes to the pathogenesis of hypoxic pulmonary hypertension. International Journal of Chronic Obstructive Pulmonary Disease, 12, 1781-1791.
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8

Western Blotting and Immunohistochemistry Protocol

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Western blotting was performed according to standard procedures. The antibodies used were: anti‐p65 (Santa Cruz, Heidelberg, Germany: sc‐109), anti‐p50 (Cell Signaling, Frankfurt, Germany: #3035), anti‐β‐tubulin (Santa Cruz: sc‐9104), anti‐AR (Merck, Vienna, Austria: 06‐680), anti‐TF (Abcam, Cambridge, UK: AB151748), anti‐IκBα (Santa Cruz: sc‐371), anti‐c‐Rel (Cell Signaling: #4727), anti‐EGR1 (Santa Cruz: sc‐110), anti‐SP1 (Cell Signaling: #9389), and anti‐GAPDH (Novus Biologicals, Littleton, CO, USA: NBP1‐47339). Immunohistochemistry was performed with a Vectastain Elite ABC horseradish peroxidase (HRP) Kit (Vectorlabs, Burlingame, CA, USA) according to the manufacturer's protocol. Antigen retrieval was performed by boiling slides for 20 min in 10 mm sodium citrate buffer (pH 6). HRP was developed with a Vectorlabs 3,3′‐diaminobenzidine peroxidase (HRP) Substrate Kit according to the manufacturer's protocol. Slides were counterstained with hematoxylin. The antibodies used for immunohistochemistry were anti‐TF (Abcam: AB151748) and anti‐EGR1 (Cell Signaling: #4154).
Hoesel B., Mussbacher M., Dikorman B., Salzmann M., Assinger A., Hell L., Thaler J., Basílio J., Moser B., Resch U., Paar H., Mackman N, & Schmid J.A. (2018). Androgen receptor dampens tissue factor expression via nuclear factor‐κB and early growth response protein 1. Journal of Thrombosis and Haemostasis, 16(4), 749-758.
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9

Western Blot Protein Detection

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Protein extraction and western blot procedure were carried out as previously reported [11] (link). After electrophoresis and blotting procedures, nitrocellulose membranes were incubated overnight with the primary antibody in a cold room (+4 °C). Membranes were then incubated with the secondary antibody (horseradish peroxidase-conjugated, 1∶3000) for 60 min (at room temperature) and the reaction was detected with a western blot detection system (GE Healthcare, Little Chalfont, UK). The antibodies used were anti-HA (Roche Applied Science, Mannheim, Germany), anti-CBX7 (sc-70232, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), anti-CBX7 (ab21873, Abcam, Cambridge, UK), anti-His-probe (sc-804, Santa Cruz Biotechnology), anti-Egr1 (sc-189, Santa Cruz Biotechnology, Inc.), anti-c-Fos (sc-52, Santa Cruz Biotechnology, Inc.), anti-Fos B (sc-48, Santa Cruz Biotechnology, Inc.), anti-Tubulin γ (sc-17787, Santa Cruz Biotechnology, Inc.) and anti-β-Actin (Clone AC-15 A5441, Sigma-Aldrich Co., St. Louis, MO).
Pallante P., Sepe R., Federico A., Forzati F., Bianco M, & Fusco A. (2014). CBX7 Modulates the Expression of Genes Critical for Cancer Progression. PLoS ONE, 9(5), e98295.
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10

Antibody Sourcing for Cellular Protein Analysis

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Anticathepsin D, anti–c-Jun, and anti–Egr-1 were from Santa Cruz; anti–Iba-1 was from Wako; anti-CD44 was from Cell Signaling; antivimentin was from Dako; anti-GAPDH, antidesmoplakin, anti– HMOX-1, anti-Prdx6, anti- ALDH1A1, and anti- IGF1 were from Abcam; antiheat-shock protein 27 (hsp27) was from Novus; anticlusterin was from Chemicon, anticystatin C was from Upstate; and anti–α-actin was from Sigma.
Kanata E., Llorens F., Dafou D., Dimitriadis A., Thüne K., Xanthopoulos K., Bekas N., Espinosa J.C., Schmitz M., Marín-Moreno A., Capece V., Shormoni O., Andréoletti O., Bonn S., Torres J.M., Ferrer I., Zerr I, & Sklaviadis T. (2019). RNA editing alterations define manifestation of prion diseases. Proceedings of the National Academy of Sciences of the United States of America, 116(39), 19727-19735.
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