IMR-5 with stable expression of ffLUC was used for bioluminescence imaging (BLI). Tumor-bearing mice were injected with d-luciferin potassium salt (150 mg/kg, intraperitoneal (IP)) and imaged on an IVIS Lumina XR System (PerkinElmer) 5 min after d-luciferin injection (1 min acquisition time). Region of interest analysis was performed using the Living Image software (PerkinElmer; V.4.3.1).
Ivis lumina xr system
Manufactured by PerkinElmer
Sourced in United States
The IVIS Lumina XR system is a non-invasive, in vivo imaging platform designed for small animal research. The system utilizes sensitive charge-coupled device (CCD) cameras to capture bioluminescent and fluorescent signals from living subjects, enabling the visualization and quantification of biological processes at the cellular and molecular level.
Automatically generated - may contain errors
Lab products found in correlation
Living image software, by PerkinElmer (3 mentions)
Xenolight dir, by PerkinElmer (1 mentions)
Living image 4, by PerkinElmer (1 mentions)
Balb c nude mice, by Charles River Laboratories (1 mentions)
Living image software package, by PerkinElmer (1 mentions)
Luciferase reporter reagent, by Promega (1 mentions)
Xenogen ivis 200 imaging system, by PerkinElmer (1 mentions)
Te2000 u, by Nikon (1 mentions)
12 protocols using ivis lumina xr system
1
In Vivo Bioluminescence Imaging
2
In Vivo Tracking of Magnetized MSCs
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Magnetized MSCs were fluorescently labeled with XenoLight DiR (PerkinElmer, Inc., Waltham, Massachusetts), a lipophilic near‐infrared fluorescent dye (absorption/emission: 748/780 nm). DiR was initially dissolved in ethanol, and this solution then mixed with PBS. MSCs were incubated with 32 μg/mL DiR solution in a 37°C incubator for 30 minutes. After incubation, the cells were centrifuged and washed with PBS to remove free dye, in accordance with the manufacturer's instructions.
In vivo tracking of MSCs was then performed using an IVIS Lumina XR system (Caliper Life Sciences/PerkinElmer, Hopkinton, Massachusetts). The filters were configured at 710 nm for excitation and 760 nm for emission. Fluorescence images were acquired immediately, 24 hours, and 48 hours after cell transplantation. At 48 hours, mice were euthanized by cervical dislocation under anesthesia and ex vivo imaging of their lungs was performed. Fluorescent signal intensities (expressed as average radiance values relative to silicotic mice without MSCs) were measured and analyzed in Living Image 4.3.1 software. Two independent experiments were performed in triplicate.
In vivo tracking of MSCs was then performed using an IVIS Lumina XR system (Caliper Life Sciences/PerkinElmer, Hopkinton, Massachusetts). The filters were configured at 710 nm for excitation and 760 nm for emission. Fluorescence images were acquired immediately, 24 hours, and 48 hours after cell transplantation. At 48 hours, mice were euthanized by cervical dislocation under anesthesia and ex vivo imaging of their lungs was performed. Fluorescent signal intensities (expressed as average radiance values relative to silicotic mice without MSCs) were measured and analyzed in Living Image 4.3.1 software. Two independent experiments were performed in triplicate.
3
Bioluminescence Optical Imaging in Mice
4
Xenograft Tumor Growth in Nude Mice
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Twenty-four-week-old male BALB/c nude mice (18–22 g) were purchased from the Beijing Vital River Laboratory Animal Technology Co., Ltd (Beijing, China) and housed in the Experimental Animal Center of Shanxi Medical University (Taiyuan, China SCXK Jin 2019-0007). The study protocol was approved by the Institutional Animal Care and Use Committee of Shanxi Medical University (IACUC SYDL2021013). Twenty nude mice were randomly divided into the NC and OE groups (n = 10 per group). The stable cell lines NC and OE (5 × 106) were subcutaneously inoculated into the left armpit of mice. The activity, diet, and mental state of the animals were observed daily. The tumor volume and weight of the mice were measured using a caliper and electronic scale every two days and calculated using the formula length × width2 × 1/2. Finally, the mice were euthanized (cervical dislocation) 40 days after the injection, and the tumors were photographed and dissociated.
To better evaluate tumor growth in nude mice, luciferase activity in tumor tissue was detected using the IVIS LuminaXR system (Perkin Elmer, Norwalk, Connecticut, USA).
To better evaluate tumor growth in nude mice, luciferase activity in tumor tissue was detected using the IVIS LuminaXR system (Perkin Elmer, Norwalk, Connecticut, USA).
5
In Vivo Tumor Targeting of IR780
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IR780, as an NIR fluorescent dye, was utilized for the visualization the BMDI enrichment in tumor model by fluorescence imaging. Before the in vivo targeting investigation, BMDI dispersion with different concentrations were applied to measure the fluorescence intensity in vitro (the excitation wavelength and the emission wavelength were 740 nm and 790 nm, respectively) by IVIS Lumina XR system (PerkinElmer)
MCF-7 tumor cells were subcutaneously inoculated on mice for tumor model established. After the tumor volume growing up to about 200 mm3, mice were administrated with free IR780 and BMDI intravenously (at an equivalent IR780 concentration of 1.5 mg/kg, based on our previous study). Fluorescence images were captured by IVIS Lumina XR system at predetermined time points of 1, 2, 8, 24 and 32 h. After 32 h, all mice were euthanatized and their organs were applied for ex vivo imaging.
MCF-7 tumor cells were subcutaneously inoculated on mice for tumor model established. After the tumor volume growing up to about 200 mm3, mice were administrated with free IR780 and BMDI intravenously (at an equivalent IR780 concentration of 1.5 mg/kg, based on our previous study). Fluorescence images were captured by IVIS Lumina XR system at predetermined time points of 1, 2, 8, 24 and 32 h. After 32 h, all mice were euthanatized and their organs were applied for ex vivo imaging.
6
In Vivo Imaging of Arthritic Joints
7
Xenograft Model of CaL-27 Tumor Suppression
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Four-week-old female BALB/c nude mice were purchased from the Shanghai Institute for Biological Sciences (Shanghai, China). Twenty BALB/c nude mice were randomly divided into two groups (n = 10/group). CaL-27 cells were transfected with either C1QTNF6-shRNA or Ctrl-shRNA lentiviral vector labeled with luciferase. Lentivirus-transfected cells (1 × 107) were inoculated into the right side of the axillary of nude mice subcutaneously. The mice were observed every 5 days. The length and width of tumors were measured using calipers, and the volume of tumors was calculated using the equation (L × W2)/2. On day 35, the animals were sacrificed, and the tumors were removed and weighed. Luciferase activity in tumor tissue was detected by IVIS LuminaXR system (Perkin Elmer, Norwalk, Connecticut, USA).
8
Bioluminescence Imaging of Tumor-Bearing Mice
9
Quantifying Bioluminescence of P. aeruginosa
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Photon emission of luminescent P. aeruginosa (PAK’ΔloxA-luxCDABE) in the mouse was measured using the IVIS Lumina XR system (Perkin Elmer), which includes an IVIS charge-coupled device camera coupled to the LivingImage software package (Perkin Elmer). Analysis of photons was done under isoflurane inhalation anesthesia. A digital false-color photon emission image of the mouse was generated, and photons were counted using a 3-min acquisition time using the following settings: Medium binning (M), Field Of View: 12.5 cm (D), f1. Image analysis and luminescence quantification have been performed with LivingImage software (Perkin Elmer). Region Of Interest (ROI) were defined as area corresponding to the surface of the chest encompassing the whole lung region after 3 min acquisition time.
10
Bioluminescent Tracking of Aspergillus Infection
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The A. fumigatus isolate Af1161 was transformed with luciferase reporter genes with the gpdA promoter as previously described with minor modifications (Brock et al., 2008 (link); Ding et al., 2013 (link)). Spores were obtained and suspended in PBS. The activity was confirmed using microplate reader (Spectra Max M2) and IVIS Lumina XR system (PerkinElmer) with a spore suspension containing 1% luciferase reporter reagent (Promega). Infected mice were introduced intraperitoneally with 100 μl D-luciferin in PBS (33.3 mg/ml) 10 min before detection. Mice were anesthetized using 2.5% isoflurane with a constant flow and then placed in an IVIS Lumina XR system chamber. Scanning was performed on days 2, 4, and 6. The region was cropped in the chest area from each animal, and the total bioluminescence signal intensity of the lung region was extracted and analyzed using Living Image 4.2 (Caliper Life Sciences).
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