Syringe pump

Manufactured by Kent Scientific

Sourced in United States

A syringe pump is a laboratory device used to precisely control the flow rate and volume of fluids delivered through a syringe. It is designed to accurately dispense small, measured quantities of liquids or solutions in a controlled and consistent manner.

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Lab products found in correlation

Hamilton syringes, by Kent Scientific (2 mentions) 30 gauge injectors, by RWD Life Science (2 mentions) Dawn heleos 2 multi angle light scattering detector, by Wyatt Technology (1 mentions) Hamilton syringe, by RWD Life Science (1 mentions) Hfe 7500, by 3M (1 mentions)

Spelling variants of this product

GenieTouch syringe pump (8 citations) Genie Plus syringe pump (2 citations)

15 protocols using syringe pump

Xenon-129 MRI Flow Phantom Study

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The flow phantom used in this study is shown in Figure 2a. The 3.175 mm tube was connected to the syringe pump (Kent Scientific Co, Torrington, CT, USA). A 30 mL syringe was filled with saline and loaded into the pump. One liter of natural abundant ¹²⁹Xe (~26%) was polarized up to 52% using the Xemed xenon polarizer and dispensed into a TedLar bag. A TedLar bag and a saline tube were connected to the membrane contactor (3M Liqui-Cel MM-0.5 × 1 Series) to mix HP Xe with saline. The outlet of the membrane contactor was connected to the 3.175 mm tube and the end of the tube was placed into the custom-built quadrature coil tuned to the Xe resonance frequency at 3T (35.33 MHz). A saline flow rate was set to 5 mL/min, 6 mL/min, 7 mL/min, and 10 mL/min.
The ¹²⁹Xe TOF GRE imaging was conducted using 20 TOF recovery times that varied from 200 ms to 2000 ms, with a step of 100 ms. The following parameters were used for imaging: TR/TE = 104.16 ms/1.22 ms, FA = 12.5°, FOV = 100 × 100 mm².

Shepelytskyi Y., Hane F.T., Grynko V., Li T., Hassan A, & Albert M.S. (2020). Hyperpolarized 129Xe Time-of-Flight MR Imaging of Perfusion and Brain Function. Diagnostics, 10(9), 630.

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Curcumin-Loaded Polymeric Nanoparticles

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NP batches were prepared by nanoprecipitation. Briefly, 60 mg of PEGylated polymer were dissolved in 3 ml acetone. For drug-loaded NP batches, curcumin was added to the organic phase at a determined curcumin/polymer ratio (from 0 to 20 % w/w). The organic phase was slowly injected with a syringe pump (Kent Scientific Corp. Torrington, CT, USA) at a rate of 1mL/min with a 26G needle in 15 mL of PBS 10mM (pH 7.4) placed in a 25 ml beaker. The aqueous phase was kept under constant stirring (530 rpm) with a magnetic agitator during the injection of the organic phase.
NPs were purified by centrifugation on a tabletop centrifuge (Multi RF centrifuge, Thermo Electron Corp. Needham heights MA, USA) to remove eventual large debris, aggregates and precipitated non-encapsulated curcumin (5 min at 5000 rpm). Supernatant was finally dialyzed against PBS during 2 h in a regenerated cellulose membrane bag, with a cut-off of 6-8000 Da (SpectraPor, Spectrum Laboratories, Rancho Dominguez, USA) to remove organic solvent residuals as well as small non-precipitated polymer chains. NPs were stored in a dark container at 4°C or were used immediately after preparation. Residual amount of non-encapsulated curcumin in solution are rapidly degraded in the aqueous phase during NP suspension storage and are thus not contributing to observed biological properties.

Rabanel J.M., Faivre J., Paka G.D., Ramassamy C., Hildgen P, & Banquy X. (2015). Effect of polymer architecture on curcumin encapsulation and release from PEGylated polymer nanoparticles: Toward a drug delivery nano-platform to the CNS. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 96.

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Targeted Manipulation of Prefrontal Cortex

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On induction day, we bilaterally microinfused (2 μg/0.5 μl/side) of Daun02 or sterile 1× PBS into the PL over a 1 min period using 10 μl Hamilton syringes and a syringe pump (Kent Scientific, Torrington, CT, USA) with polyethylene-50 tubing to 30-gauge injectors (RWD Life Science, Guangdong, China) that extended 1 mm below the guide cannula. Injectors were left in the cannula for 1 min after infusion before being removed.

Sortman B.W., Gobin C., Rakela S., Cerci B, & Warren B.L. (2022). Prelimbic Ensembles Mediate Cocaine Seeking After Behavioral Acquisition and Once Rats Are Well-Trained. Frontiers in Behavioral Neuroscience, 16, 920667.

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Monocyte Adhesion Assay in Arterial Chip

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Heparinized mouse whole blood was added to the artery-chip chamber reservoir and withdrawn through a single channel using a syringe pump (Kent Scientific) at a flow rate resulting in a physiological shear stress of 2 dynes/cm² at the glass fluid interface (a wall shear stress corresponding with athero-prone regions in the arterial vasculature).(20 (link)) Arrested monocytes were fixed with 1 × Lyse/Fix Buffer (Biolegend) and the coverslip was removed from the PDMS chamber and then incubated with hanks balanced salt solution (HBSS) containing 1% human serum albumin (HSA) for 30 minutes at room temperature. Coverslips were then washed twice with Dubelccos Phosphate Buffer Solution 1× stained with PE anti-CD115 and BODIPY.

Foster G.A., Xu L., Chidambaram A.A., Soderberg S.R., Armstrong E.J., Wu H, & Simon S.I. (2015). CD11c/CD18 signals very late antigen-4 activation to initiate foamy monocyte recruitment during the onset of hypercholesterolemia. Journal of immunology (Baltimore, Md. : 1950), 195(11), 5380-5392.

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Multi-Angle Light Scattering Analysis of Copolymer Solutions

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A 5.0 mg/mL stock solution
of each polymer [i.e., PVA-comb-PLLA,
PVA-comb-PDLLA, PVA-comb-(PLLA-b-PDLLA), and PVA-comb-(PDLLA-b-PLLA)] in THF was filtered through a 0.2 μm PTFE syringe filter
and then diluted with the same solvent in order to produce five concentrations
ranging between 1.5 and 5.0 mg/mL. 1.5 mL of each solution was injected
into a DAWN HELEOS II multi-angle light scattering detector, operating
at 660 nm and 25 °C (Wyatt Technology, US), using a syringe pump
(Kent Scientific Corporation, US). Software Astra version 7.1.4.8
(Wyatt Technology, US) was used to collect and analyze the static
light scattering (SLS) data (Zimm plots; see Supporting Information, Figure S5) and obtain the weight-average MW, radius
of gyration (R_g), and second virial coefficient
(A2) using the nominal concentration of the solutions and the dn/dc data previously obtained for the polymers
with the same composition analyzed by GPC/SEC. All samples were analyzed
using the Debye formalism with the fit degree equal to 1 for both
the angle and concentration.

Scoponi G., Francini N., Paradiso V., Donno R., Gennari A., d’Arcy R., Capacchione C., Athanassiou A, & Tirelli N. (2021). Versatile Preparation of Branched Polylactides by Low-Temperature, Organocatalytic Ring-Opening Polymerization in N-Methylpyrrolidone and Their Surface Degradation Behavior. Macromolecules, 54(20), 9482-9495.

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Ion Mobility Spectrometry Analysis of Phthalates

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As a drift gas in IMS, we used zero air generated by a MaSa Tech zero air generator and an additional moisture trap (Agilent) resulting in water concentration of ~20 ppm. In case of sample flow, non-purified lab air was constantly sucked in, while the IMS was operated in sub-atmospheric pressure. The sample flow rate was controlled by a micro-splitter valve (Supelco) accompanied by a capillary and a gas flow meter (Platon). The phthalates were supplied from Sigma-Aldrich with the following purities: dimethyl isophthalate 99%, dimethyl phthalate 99%, dimethyl terephthalate 99%.
The vapours of the phthalates were introduced into the reaction region of the IMS through a sample inlet. The phthalates were placed in a glass syringe (about 0.5 g) and we waited at least 30 min to achieve an equilibrium between the gas and the solid or liquid phase. Afterwards, the syringe was connected via a capillary with the sample inlet and using a syringe pump (Kent Scientific), the sample was introduced into the reaction region of the IMS with a pre-set flow rate.

Michalczuk B., Moravský L., Papp P., Mach P., Sabo M, & Matejčík Š. (2019). Isomer and conformer selective atmospheric pressure chemical ionisation of dimethyl phthalate. Physical chemistry chemical physics : PCCP, 21(25).

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Multicomponent Fibrous Scaffold Fabrication

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PHBV was dissolved in a 90:10 DCM methanol ratio followed by the addition and dissolution of Poly-l-Lactic Acid for a composite blend formation. Each bilayer formulation has been named bilayer 90:10, bilayer 80:20, and bilayer 70:30, where 90:10 means weight by weight distribution of 90 against 10 between PHBV and PLLA, respectively. The solution was prepared at room temperature on a mechanical stirrer overnight. On the following day, 3 syringes were filled with 5 mL solutions and were fitted in the multichannel needle holder. The needle gauge was 18 mm in diameter. The syringes were fixed on the (Kent Scientific, Torrington, CT, USA) syringe pump, having a flow rate ranging from 0.35 to 0.45 mL/h. The applied voltage was 15kV with a high-voltage power supply (Genvolt, Bridgnorth, UK). The fibrous scaffold was collected on a rotating drum 16 cm × 6 cm in diameter and wrapped with aluminum foil at a distance of 14 cm from the needle tips with a rotation speed of 300 rpm [34 (link)]. An amount of 15 mL of the polymer solution was dispensed to make a fibrous sheet, as described by Bye et al. [46 (link)]. Figure 9 shows the schematic fabrication of membranes.

Jamal M., Sharif F., Shozab Mehdi M., Fakhar-e-Alam M., Asif M., Mustafa W., Bashir M., Rafiq S., Bustam M.A., S.a.i.f.-.u.r.-.R.e.h.m.a.n., Dahlous K.A., Shibl M.F, & Al-Qahtani N.H. (2024). Development of Biocompatible Electrospun PHBV-PLLA Polymeric Bilayer Composite Membranes for Skin Tissue Engineering Applications. Molecules, 29(9), 2049.

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Precise Intracranial Injections Using Syringe Pump

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We performed intracranial injections using a syringe pump (Kent Scientific) and 10 μl Hamilton syringes that were attached via polyethylene-50 tubing to 30-gauge injectors (RWD) that extended 1 mm beyond the guide cannula. We infused 0.5 μl over 1 min and left the injectors in place for an additional 1 min before removal.

Quintana-Feliciano R., Gobin C., Kane L., Sortman B., Rakela S., Genovese A., Tunstall B., Caprioli D., Iñiguez S.D, & Warren B.L. (2021). Food-Seeking Behavior Is Mediated by Fos-Expressing Neuronal Ensembles Formed at First Learning in Rats. eNeuro, 8(2), ENEURO.0373-20.2021.

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Intracranial Infusion of Daun02 Using Syringe Pump

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In Experiments 2 and 4, we performed intracranial infusions using a syringe pump (Kent Scientific) and 10-μl Hamilton syringes that were attached via polyethylene tubing to 30-gauge injectors (RWD) that extended 1 mm beyond the guide cannula. We infused 0.5 μl of Daun02 or vehicle over 1 min and left the injectors in place for an additional 1 min before removal, as previously described.^{20 (link),24 (link),28 (link)}

Gobin C., Sortman B., Rakela S., Quintana-Feliciano R, & Warren B.L. (2022). Fos-expressing neuronal ensembles in rat infralimbic cortex encode initial and maintained oxycodone seeking in rats. Addiction biology, 27(2), e13148.

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Platelet Activation in Flow Chambers

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The flow chambers were assembled by inverting the PDMS molded flow chamber onto the stamped Nexterion-H glass slide. Reversible sealing held the PDMS in place strongly enough to prevent leakage for the duration of the experiments. The flow chambers were connected to a syringe pump (Kent Scientific, Torrington, CT, USA) using polytetrafluorethylene tubing (shear rate 39 s⁻¹, PTFE, Cole-Parmer, Vernon Hills, IL, USA,). PTFE tubing was kept as short as possible to minimize any effects it might have on platelet activation. After assembly, a solution of human serum albumin (HSA, 1 mg/mL in PBS, Sigma Aldrich, St. Louis, MO, USA) was perfused through each flow cell and was allowed to incubate at room temperature for 1 hr. Covalently attached HSA served to passivate the unreacted regions of the glass slide, the walls of each flow chamber and PTFE tubing by physical adsorption.

Rahman S., Eichinger C, & Hlady V. (2018). Effects of Upstream Shear Forces on Priming of Platelets for Downstream Adhesion and Activation. Acta biomaterialia, 73, 228-235.

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