Purified mouse NG2-cell cultures were prepared from P1-3 CD1 pups, as previously described 58 (link),59 (link). NG2-cells were plated onto poly-L-lysine-coated coverslips and treated with 10 ng/ml platelet-derived growth factor (PDGF) (human AB, heterodimer form; Upstate Biotechnology, Lake Placid, NY). After 24 hours, cultures were treated with the GABAA receptor agonist muscimol (100 μM) (abcam) or with saline, both in the presence of the AMPA-type glutamate receptor antagonist NBQX (50 μM) (Tocris) and the NMDA receptor antagonist D-AP5 (20 μM) (abcam). In some cases bicuculline (50 μM) was applied 1 hour prior to the administration of muscimol. After 18 hours the cells were incubated with BrdU (30 μM) (Sigma) and fixed using 4% PFA 6 hours later.
Gabaa receptor agonist muscimol
Manufactured by Abcam
GABAA receptor agonist muscimol is a chemical compound used in scientific research. It acts as an agonist, or activator, of the GABAA receptor, which is an important neurotransmitter receptor in the central nervous system.
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Lab products found in correlation
2 protocols using gabaa receptor agonist muscimol
1
NG2-cell culture and GABAA receptor stimulation
2
NG2-cell culture and GABAA receptor stimulation
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Purified mouse NG2-cell cultures were prepared from P1-3 CD1 pups, as previously described 58 (link),59 (link). NG2-cells were plated onto poly-L-lysine-coated coverslips and treated with 10 ng/ml platelet-derived growth factor (PDGF) (human AB, heterodimer form; Upstate Biotechnology, Lake Placid, NY). After 24 hours, cultures were treated with the GABAA receptor agonist muscimol (100 μM) (abcam) or with saline, both in the presence of the AMPA-type glutamate receptor antagonist NBQX (50 μM) (Tocris) and the NMDA receptor antagonist D-AP5 (20 μM) (abcam). In some cases bicuculline (50 μM) was applied 1 hour prior to the administration of muscimol. After 18 hours the cells were incubated with BrdU (30 μM) (Sigma) and fixed using 4% PFA 6 hours later.
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