Anti cd31 alexa fluor 488

Manufactured by BioLegend

Anti-CD31-Alexa Fluor 488 is a fluorescently labeled antibody that targets the CD31 antigen, also known as PECAM-1. CD31 is a cell adhesion molecule expressed on the surface of endothelial cells, platelets, and some immune cells. The Alexa Fluor 488 dye is conjugated to the antibody, allowing for detection and visualization of CD31-expressing cells using fluorescence-based techniques.

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Lab products found in correlation

Collagen 1, by Cedarlane (1 mentions) Anti cd34 fitc, by BioLegend (1 mentions) Hsp47, by Abcam (1 mentions) Anti cd45 alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Anti ncam biotin, by BioLegend (1 mentions) Laminin, by Abcam (1 mentions) Anti cd29 apc, by BioLegend (1 mentions) Live dead fixable red dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Anti cd4 pe cy5, by BioLegend (1 mentions) Anti cd45ra apc cy7, by BioLegend (1 mentions) Alexa fluor 700, by BioLegend (1 mentions) Anti ccr7 bv785, by BioLegend (1 mentions) Anti cd19 bv711, by BioLegend (1 mentions) Anti cd14 bv711, by BD (1 mentions) Anti cd3 bv510 hit3a, by BD (1 mentions) Anti ki 67 pe cy7, by Thermo Fisher Scientific (1 mentions) Anti foxp3 pe, by Thermo Fisher Scientific (1 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Alexa Fluor 488 (61 citations) Anti-CD31 (45 citations) Alexa 488 (13 citations) Alexa Fluor 488 anti-human CD31 antibody (2 citations) Anti-mouse CD31-Alexa Fluor® 488 (2 citations)

2 protocols using anti cd31 alexa fluor 488

Antibody Panel for Cell Characterization

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The following antibodies were used in this study. The source of each antibody is indicated. Rabbit::H3K9me3 (Abcam #ab8898 1:4000), LAP2α (Abcam #ab5162 1:500), SIRT1 (Abcam #ab7343 1:200); Rabbit: Mouse: HP1γ (Millipore Sigma #05-690 1:200), Lamin A/C (Abcam #ab40567 1:200), GFP (Invitrogen, #A11122, 1:250); Luciferase (Sigma-Aldrich, #L0159, 1:200); Collagen I (Cedarlane Labs, #CL50151AP, 1:200); HSP47 (Abcam, #ab77609, 1:200), Laminin (Abcam, #AB11576, 1:1000), anti-CD31-Alexa Fluor 488 (clone WM59; BioLegend; #303110, 1:75), anti-CD45-Alexa Fluor 488 (clone HI30; Invitrogen; #MHCD4520, 1:75), anti-CD34-FITC (clone 581; BioLegend; #343503, 1:75), anti-CD29-APC (clone TS2/16; BioLegend; #303008, 1:75) and anti-NCAM-Biotin (clone HCD56; BioLegend; #318319, 1:75), anti-CD31-Alexa Fluor 488 (clone WM59; BioLegend; #303110, 1:75), anti-CD45-Alexa Fluor 488 (clone HI30; Invitrogen; #MHCD4520, 1:75), anti-CD34-FITC (clone 581; BioLegend; #343503, 1:75), anti-CD29-APC (clone TS2/16; BioLegend; #303008, 1:75), and anti-NCAM-Biotin (clone HCD56; BioLegend; #318319, 1:75).

Sarkar T.J., Quarta M., Mukherjee S., Colville A., Paine P., Doan L., Tran C.M., Chu C.R., Horvath S., Qi L.S., Bhutani N., Rando T.A, & Sebastiano V. (2020). Transient non-integrative expression of nuclear reprogramming factors promotes multifaceted amelioration of aging in human cells. Nature Communications, 11, 1545.

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Multiparametric Flow Cytometry Analysis

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The following anti-human fluorochrome-conjugated antibodies were used: Alexa Fluor 700-conjugated antibody to human CD3 (UCHT1); anti-IL-7Rα-BV421 (A019D5), anti-CD4-PE-Cy5 (OKT4), anti-CD45RA-APC-Cy7 (HI100), anti-Fas-BV650 (DX2), anti-CCR7-BV785 (G043H7), anti-CD31-Alexa Fluor 488 (WM59), anti-TCR γ/δ-Percp-Cy5.5 (B1), anti-CD25-BV650 (BD96; BioLegend), anti-CD19-BV711 (SJ25C1), anti-CD14-BV711 (MφP9), anti-CD3-BV510 (HIT3a), anti-CD8-Alexa Fluor 700 (RPA-T8; all from BD Biosciences); anti-CD19-PE-eFluor610 (HIB19); anti-CD14-PE-eFluor610 (61D3), and anti-CD56-APC (CMSSB; Thermo Fisher). Simultaneously, dead cells were stained using a Live/Dead Fixable Red Dead Cell Stain Kit (Thermo Fisher). The cells were fixed and permeabilized using a Foxp3 Staining Buffer Set (Thermo Fisher), and then intracellular molecules were stained (4°C, 20 minutes) using anti-Bcl-2-Alexa Fluor 488 (100; BioLegend), anti-Ki-67-PE-Cy7 (20Raj1), and anti-Foxp3-PE (236A/E7; Thermo Fisher). Detailed information about the multicolor flow cytometry panel used in this study is shown in supplemental Table 4.

Kim S., Lee S.W., Koh J.Y., Choi D., Heo M., Chung J.Y., Lee B.H., Yang S.H., Sung Y.C., Lee H., Shin E.C, & Park S.H. (2022). A single administration of hIL-7-hyFc induces long-lasting T-cell expansion with maintained effector functions. Blood Advances, 6(23), 6093-6107.

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