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Ru dpp 3 cl2

Manufactured by Merck Group
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Sourced in China

[Ru(dpp)3]Cl2 is a ruthenium-based compound that serves as a photosensitizer. Its core function is to absorb light and transfer energy, which can be utilized in various applications. The detailed description and intended use of this product require further technical information that is not available within the scope of this request.

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Lab products found in correlation

Peg nh2, by Nanocs (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Bismuth nitrate pentahydrate, by Merck Group (1 mentions) 2 7 dichlorodihydrofluorescein diacetate, by Merck Group (1 mentions) Alexa fluor 647 mouse anti h2ax ps139, by BD (1 mentions) Glutathione (gsh), by Merck Group (1 mentions) Dulbecco s minimum essential medium, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Mcf 10a, by American Type Culture Collection (1 mentions) Hepg2, by American Type Culture Collection (1 mentions) Hek293, by American Type Culture Collection (1 mentions) Hl 7702, by American Type Culture Collection (1 mentions) Trypsin edta, by Nanocs (1 mentions) 9 10 anthracenedipropanoic acid, by Merck Group (1 mentions) 3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions) Dihydrorhodamine 123 dhr123, by Merck Group (1 mentions) Rpmi medium, by Thermo Fisher Scientific (1 mentions) N methylpyrrolidone, by Merck Group (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions)

5 protocols using ru dpp 3 cl2

1

Nanoparticle-based Apoptosis Detection

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Bismuth nitrate pentahydrate [Bi(NO3)3·5H2O], sodium chloride (NaCl), potassium borohydride (KBH4), hydrogen dioxide (H2O2), 2,7-dichlorodihydrofluorescein diacetate, MB, GSH, [Ru(dpp)3]Cl2, and DTNB were obtained from Sigma-Aldrich. PEG-NH2 [molecular weight (MW), 5000] and Cy5.5-PEG-NH2 (MW, 5000) were provided by Nanocs Inc. Trypsin-EDTA, Dulbecco’s minimum essential medium, RPMI 1640 medium, fetal bovine serum, and PBS (pH 7.4) were provided by Gibco Life Technologies. Alexa Fluor 647 mouse anti-H2AX (pS139) and anti-cleaved poly(adenosine 5′-diphosphate–ribose) polymerase (Asp214) antibodies were secured from BD Pharmingen. Normal human liver cells (HL-7702; catalog no. 77402), human embryonic kidney cells (HEK293, catalog no. CRL-1573), normal human mammary epithelial cells (MCF-10A, catalog no. CRL-10781), human breast cancer cell (MCF-7, catalog no. HTB-22), and human hepatoma cells (HepG2, catalog no. HB-8065) were supplied by the American Type Culture Collection (ATCC). ATCC used morphology, karyotyping, and polymerase chain reaction–based approaches to confirm the identity of human cell lines and rule out intra- and interspecies contamination. Also, the cell line was frequently evaluated through its morphological features.
Chen L., Mao Z., Wang Y., Kang Y., Wang Y., Mei L, & Ji X. (2022). Edge modification facilitated heterogenization and exfoliation of two-dimensional nanomaterials for cancer catalytic therapy. Science Advances, 8(39), eabo7372.
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2

Ruthenium Complex-Based Analytical Procedures

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Analytical grade KMnO4, sodium dodecyl sulfate (SDS), concentrated sulfuric acid (H2SO4), hydrogen peroxide (H2O2) were obtained from Beijing Chemicals Reagents. [Ru(dpp)3]Cl2 was purchased from Sigma-Aldrich Co. (Shanghai, China). MilliQ water was used throughout. All other chemical reagents were of analytical reagent grade. The citrate buffer was prepared by mixing an approximate ratio of citric acid and sodium citrate solutions.
Zhang Y., Hu K., Ling Z, & Di W. (2019). A MnO2–[Ru(dpp)3]Cl2 system for colorimetric and fluorimetric dual-readout detection of H2O2. RSC Advances, 9(14), 7803-7810.
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3

Vermiculite-Based Nanoparticle Synthesis

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Vermiculite was supplied from Yunnan province, China. Cy7-PEG-NH2 (MW: 5k) and PEG-NH2 (MW: 5k) were purchased from Nanocs Inc. PBS (pH 7.4 and 5.5), DMEM medium, RPMI medium, trypsin-EDTA, and fetal bovine serum (FBS) were purchased from Gibco Life Technologies. 9,10-anthracenedipropanoic acid (ABDA), [Ru(dpp)3]Cl2 (RDPP), H2O2 (30%), N-methyl-pyrrolidone (NMP), methylene blue (MB), 3,3′,5,5′-tetramethylbenzidine (TMB), glutathione, 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), and dihydrorhodamine 123 (DHR123) were supplied by Sigma-Aldrich.
Ji X., Ge L., Liu C., Tang Z., Xiao Y., Chen W., Lei Z., Gao W., Blake S., De D., Shi B., Zeng X., Kong N., Zhang X, & Tao W. (2021). Capturing functional two-dimensional nanosheets from sandwich-structure vermiculite for cancer theranostics. Nature Communications, 12, 1124.
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4

Oxygen Generation Efficiency Evaluation

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The O2 generation efficiency in cells was investigated with an O2 sensing probe, [(Ru(dpp)3)]Cl2 (Sigma–Aldrich, Co. Ltd.). The fluorescence of this agent could be strongly quenched by O2. RAW264.7 cells were cultured at a density of 1 × 104 cells per well in 96‐well plate and under hypoxia condition for 24 h. Then, the cells were incubated with O2 sensing probe for 4 h and with 40 µg mL−1 of MnO2 NPs, MSN@Ce, MnO2 + Ce, and MnO2‐motors for another 4 h. The fluorescent signal of [Ru(dpp)3]Cl2 in cells was then observed and photographed under inverted fluorescence microscopy.
Xu C., Jiang Y., Wang H., Zhang Y., Ye Y., Qin H., Gao J., Dan Q., Du L., Liu L., Peng F., Li Y, & Tu Y. (2022). Arthritic Microenvironment Actuated Nanomotors for Active Rheumatoid Arthritis Therapy. Advanced Science, 10(4), 2204881.
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5

Measurement of Intracellular Oxidative Stress

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M1 macrophages were treated with blank, ZIF-8, or 8% Ce@ZIF-8 NPs for 24 h. The dichloro-dihydrofluorescein diacetate (DCFH-DA) probe (Beyotime Biotechnology) and the O 2 indicator [Ru(dpp) 3 ]Cl 2 (Sigma) were utilized to measure the levels of H 2 O 2 and O 2 , respectively. After adding with DCFH-DA (10 μM) or [Ru(dpp) 3 ]Cl 2 (2 μM), M1 macrophages were incubated in the dark at 37 °C for 30 min, and images were captured using confocal microscopy. A hydrogen peroxide assay kit (Beyotime, China) was used to detect the quantitative level of intracellular H 2 O 2 .
Yan Q., Shi S., Ge Y., Wan S., Li M, & Li M. (2023). Nanoparticles of Cerium-Doped Zeolitic Imidazolate Framework-8 Promote Soft Tissue Integration by Reprogramming the Metabolic Pathways of Macrophages. ACS biomaterials science & engineering, 9(7).
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