Bioluminescence assays were performed by pipetting 10 μL of sample into a black-walled 96-well plate (Corning) and adding 1000 μL of coelenterazine native substrate (NanoLight Technology) at 100 μmol/L diluted in phosphate buffered saline. Samples were read immediately using a BioTek Synergy 2 Multi- Mode Reader for 10s at a gain of 100–200 (BioTek).
Coelenterazine native substrate
Manufactured by Nanolight Technology
Coelenterazine native substrate is a chemical compound that serves as a substrate for various bioluminescent enzymes. It is the natural substrate for the luciferase enzymes that produce bioluminescence in marine organisms such as jellyfish and sea pansy. The core function of coelenterazine is to enable the production of light through bioluminescent reactions when combined with the appropriate enzymes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using coelenterazine native substrate
1
Bioluminescence Assay Protocol
2
Bioluminescence Assay Protocol
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