Proscan 3

Manufactured by Nikon

The ProScan III is a high-performance motorized microscope stage from Nikon. It provides precise and repeatable positioning of specimens for accurate imaging and analysis. The ProScan III features a range of travel, resolution, and speed options to meet the requirements of a variety of laboratory applications.

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Lab products found in correlation

Ti inverted widefield microscope, by Nikon (1 mentions) 4.2 plus scmos monochrome camera, by Oxford Instruments (1 mentions) Orca flash4.0 v3 digital cmos camera, by Hamamatsu Photonics (1 mentions)

Spelling variants of this product

H101F—ProScan III Open Frame Upright Motorized H101F Flat Top Microscope Stages (3 citations)

2 protocols using proscan 3

Confocal Imaging of Retinal Specimens

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Immunostained retinas were images on Olympus FV300 or Nikon A1 confocal laser
scanning microscopes. For experiments with reported retinal eccentricities, a motorized
translational stage (Prior ProScan III) integrated with the Nikon A1 microscope was used
to record coordinates of the ONH and each field of view in order to calculate distance
from the ONH.

Wang J., O’Sullivan M.L., Mukherjee D., Puñal V.M., Farsiu S, & Kay J.N. (2017). Anatomy and spatial organization of Müller glia in mouse retina. The Journal of comparative neurology, 525(8), 1759-1777.

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Fluorescent Retina Imaging Protocols

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Fluorescent flatmount images were acquired with Nikon Ti inverted widefield microscope with a Prior ProScanIII motorized stage. The objective used was Plan Apo Lambda 10 x/0.45 Air DIC N1 objective, and the camera used was Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera. Fluorescent retina section images were acquired with W1 Yokogawa Spinning disk confocal microscope with 50 µm pinhole disk and 488, 561, and 640 laser lines. The objectives used were either Plan Apo 20 x/0.75 air or Plan Apo 60 x/1.4 oil objectives, and the camera used was Andor Zyla 4.2 Plus sCMOS monochrome camera. Nikon Elements Acquisition Software (AR 5.02) was used for image acquisition and Fiji or Adobe Photoshop CS6 was used for image analysis.

Amamoto R., Wallick G.K, & Cepko C.L. (2022). Retinoic acid signaling mediates peripheral cone photoreceptor survival in a mouse model of retina degeneration. eLife, 11, e76389.

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