Cell proliferation was measured by using the WST-1 assays (Roche) according to the manufacturers’ instructions. Flow cytometry was used for quantitative assessment of apoptosis using the FITC-Annexin V/propidium iodide (PI) Apoptosis Detection Kit I (BD Pharmingen).
Fitc annexin 5 propidium iodide apoptosis detection kit 1
Manufactured by BD
Sourced in United States
The FITC-Annexin V/propidium iodide (PI) Apoptosis Detection Kit I is a laboratory tool used to identify and quantify apoptosis, a type of programmed cell death. The kit contains FITC-labeled Annexin V and propidium iodide, which are used as fluorescent markers to detect phosphatidylserine exposure and cell membrane integrity, respectively.
Automatically generated - may contain errors
Lab products found in correlation
Wst 1 assay, by Roche (5 mentions)
Facs calibur 4 color flow cytometer, by BD (2 mentions)
Accuri c6, by BD (1 mentions)
Fc500, by Beckman Coulter (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
Cellquest software, by BD (1 mentions)
Facs lsr 2, by BD (1 mentions)
Flow cytometry, by BD (1 mentions)
15 protocols using fitc annexin 5 propidium iodide apoptosis detection kit 1
1
Cell Proliferation and Apoptosis Assay
2
Cell Proliferation and Apoptosis Assays
3
Cell Viability and Apoptosis Assays
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Cell viability was assessed using MTT assays for assessment of proliferative capacity, and flow cytometry for quantitative assessment of apoptosis. The standard MTT assays were performed as described previously [9 (link)]. For flow cytometry, the FITC-Annexin V/propidium iodide (PI) Apoptosis Detection Kit I (BD Pharmingen) were used according to the manufacturer’s instructions.
4
Quantifying Cell Cycle and Apoptosis
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Flow cytometry was used for the quantitative assessment of cell cycle and apoptosis [44 (link)]. Briefly, to measure cell cycle response, PEL cell pellets were fixed in 70% ethanol, and incubated at 4°C overnight. Cell pellets were re-suspended in 0.5 mL of 0.05 mg/mL PI plus 0.2 mg/mL RNaseA, and incubated at 37°C for 30 min. Cell cycle distribution was analyzed using a BD Accuri C6 flow cytometer. Apoptosis was assessed by the FITC-Annexin V/propidium iodide (PI) Apoptosis Detection Kit I (BD Pharmingen, San Jose, California, USA) on a flow cytometer. Data was normalized as the fold change compared to the DMSO control.
5
Cell Proliferation and Apoptosis Assay
6
Cell Proliferation and Apoptosis Assay
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MTT assays were used for assessment of proliferative capacity as described previously [47] (link). Flow cytometry was used for quantitative assessment of apoptosis using the FITC-Annexin V/propidium iodide (PI) Apoptosis Detection Kit I (BD Pharmingen) according to the manufacturer’s instructions.
7
Apoptosis Quantification by Flow Cytometry
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Apoptosis was quantified by flow cytometry using the FITC-Annexin V/propidium iodide (PI) Apoptosis Detection Kit I (BD Pharmingen) according to the manufacturer's instructions. Data were collected using a FACS Calibur 4-color flow cytometer (BD Bioscience).
8
Quantifying Apoptosis and Cell Cycle
9
Cell Proliferation and Apoptosis Analysis
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Cell proliferation was determined by using the WST-1 assays (Roche) according to the manufacturer's instructions. Briefly, after the period of treatment of cells, 10 μL/well of cell proliferation reagent, WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitro-phenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate), was added into 96-well microplate and incubated for 3 h at 37 °C in 5% CO2. The absorbance of samples was measured by using a microplate reader at 490 nm. Flow cytometry was used to the quantitative analysis of apoptosis with the FITC-Annexin V/propidium iodide (PI) Apoptosis Detection Kit I (BD Pharmingen).
10
Cell Viability and Apoptosis Analysis
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Cell viability was assessed using MTT assays for assessment of proliferative capacity, and flow cytometry was used for quantitative assessment of apoptosis. Standard MTT assays were performed as described previously [5 (link)]. For flow cytometry, the FITC-Annexin V/propidium iodide (PI) Apoptosis Detection Kit I (BD Pharmingen) was used according to the manufacturer's instructions.
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