Mouse melanoma B16 cells (Cat. No. TCM 2) and human melanoma A375 cells (Cat. No. SCSP-533) from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences China (Shanghai, China) were cultured in a sterile cell culture chamber (HF240, HEALFORCE, Shanghai Lishen Scientific Equipment Co. Ltd.) at 37°C with 95% air and 5% CO2 with saturated humidity. B16 cells were cultured in 1,640 medium (Cat. No. SH30809.01, Hyclone) supplemented with 10% FBS (Cat. No. 10091-148, Gibco) and 1% streptomycin solution (Cat. No. P1400, Solarbio). A375 cells were cultured in DMEM high glucose medium (Cat. No. SH30022.01, Hyclone) supplemented with 10% FBS, 1% streptomycin solution, and 1% sodium pyruvate (Cat. No. SP0100, Solarbio). Similarly, the normal human keratinocyte cell line HaCaT cells (Cat. No. KG300, KeyGEN BioTECH) were cultured in a DMEM high glucose medium supplemented with 10% FBS and 1% streptomycin solutions.
Hf240
Manufactured by Heal Force
Sourced in China, Israel
The HF240 is a laboratory centrifuge that is designed for general-purpose applications. It features a compact and durable construction, and is capable of achieving a maximum speed of 4,000 rpm. The HF240 is equipped with a fixed-angle rotor that can accommodate multiple sample tubes or microplates.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Streptomycin mixture, by Solarbio (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Sodium pyruvate, by Solarbio (1 mentions)
Trypsin, by Yeasen (1 mentions)
Penicillin, by Sartorius (1 mentions)
Streptomycin, by Sartorius (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Mc3t3 e1 cells, by Cyagen (1 mentions)
6 protocols using hf240
1
Culturing Melanoma and Keratinocyte Cells
2
Cultivation and Characterization of Human and Mouse Melanoma Cell Lines
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Human melanoma A375 cells (Cat no. SCSP-533) and mouse melanoma B16 cells (Cat no. TCM 2) were obtained from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences China (Shanghai, China). The cells were cultured in a sterile cell culture chamber (HF240, HEALFORCE, Shanghai Lishen Scientific Equipment Co. Ltd.) with 95% air and 5% CO2 saturated humidity at a temperature of 37 °C. Human melanoma A375 cells were subsequently cultured and tested using DMEM high glucose medium (Cat no. SH30022.01, Hyclone) supplemented with 10% FBS (Cat no. 10091-148, Gibco), 1% streptomycin mixture (Cat no. P1400, Solarbio), and 1% sodium pyruvate (Cat no. SP0100, Solarbio). Mouse B16 cells were cultured in 1640 medium (Cat no. SH30809.01, Hyclone) supplemented with 10% FBS and 1% streptomycin mixture.
3
Bacterial Culture and Suspension Preparation
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Gram‐negative E. coli, gram‐positive S. aureus and MRSA were inoculated into nutrient agar by streak plate method. After cultured in a constant temperature incubator (HF‐240, HEAL FORCE) for 24 h, colonies were transferred to 25 mL sterile Luria‐Bertani (LB) broth medium at 37 °C and cultured to mid‐log phase. After centrifugation and washing for three times, bacterial suspensions with different concentration in PBS were prepared. The concentrations of bacterial suspensions were estimated by flat colony counting method.
4
Culturing Human Tongue Squamous Cell Carcinoma
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Human tongue squamous cell carcinoma Cal-27 and Tca8113 cells were purchased from Yantai Bayu Biotechnology Co. Ltd. The Cal-27 cells were grown on 89% DMEM with 1% penicillin/streptomycin and 10% FBS. The Tca8113 cells were grown on RPMI-1640 medium containing 10% FBS and 1% penicillin/streptomycin. The cells were incubated in a sterile incubator (HF240, HEALFORCE, Shanghai, China). After the cells had grown to about 80% of the culture flask, passaging was carried out, and the passage was carried out once in a ratio of 3 : 1 in about 2-3 days.
5
Culturing Human Endometrial Adenocarcinoma Cells
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Human endometrial adenocarcinoma cell lines Ishikawa and HEC-1-a were purchased from Shanghai Bogoo Biotechnology. Co., Ltd. The cells were cultured in a RPMI1640 (Hyclone, Thermo) solution containing 10% fetal calf serum (Hyclone, Thermo) and incubated in a saturated humidity incubator (HF240, Heal Force) at 37°C with 5% CO2. The experiments were carried out when the cells reached the logarithmic growth phase.
6
Culturing MC3T3-E1 Osteoblast Cells
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MC3T3-E1 cells were purchased from Cyagen Biosciences Inc. (M7-0201, Guangzhou, China). Cells were cultured with Minimum Essential Medium Alpha (α-MEM, HyClone, Utah, USA) containing 10% fetal bovine serum (FBS) (BioInd, Israel), 100 U/mL streptomycin, and 100 U/mL penicillin (BioInd, Israel) in an incubator (Heal Force, HF-240) at 37 °C and 5% CO2. When the cell density reached 70–80%, it was digested with 0.25% trypsin (Yeasen) and then passaged.
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