The largest database of trusted experimental protocols

Alexafluor 647 labeled ssdna oligonucleotides

Manufactured by Integrated DNA Technologies

AlexaFluor™ 647-labeled ssDNA oligonucleotides are single-stranded DNA molecules labeled with the AlexaFluor™ 647 fluorescent dye. They are designed for use in various molecular biology and biochemical applications that require the detection or tracking of DNA sequences.

Automatically generated - may contain errors

2 protocols using alexafluor 647 labeled ssdna oligonucleotides

1

Fluorescent DNA Oligonucleotide Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ssDNA oligonucleotides and AlexaFluor 647-labeled ssDNA oligonucleotides were purchased from Integrated DNA Technologies Inc. Full oligonucleotide sequences can be found in the accompanying Supporting Information (SI) file. Ultrapure equimolar deoxynucleotide triphosphate (dNTP) sodium salt solution was purchased from New England Biolabs Inc. (Catalog #N0447S) as an equimolar mixture of 10 mM dATP, dCTP, dGTP and dTTP giving a final dNTP concentration of 40 mM. AlexaFluor 647 C2 maleimide (Free AF) was purchased from ThermoFisher Scientific (Catalog #A20347). All other chemicals including 97% 1, 1′-diethyl-2, 2′-cyanine iodide (PIC, CAS #:977-96-8); molecular biology-grade water; sodium chloride (NaCl); magnesium chloride (MgCl2); and TRIS (2-Amino-2-(hydroxymethyl)propane-1, 3-diol; pH 7.0) were sourced from Sigma-Aldrich.
+ Open protocol
+ Expand
2

Fluorescent DNA Oligonucleotide Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ssDNA oligonucleotides and AlexaFluor 647-labeled ssDNA oligonucleotides were purchased from Integrated DNA Technologies Inc. Full oligonucleotide sequences can be found in the accompanying Supporting Information (SI) file. Ultrapure equimolar deoxynucleotide triphosphate (dNTP) sodium salt solution was purchased from New England Biolabs Inc. (Catalog #N0447S) as an equimolar mixture of 10 mM dATP, dCTP, dGTP and dTTP giving a final dNTP concentration of 40 mM. AlexaFluor 647 C2 maleimide (Free AF) was purchased from ThermoFisher Scientific (Catalog #A20347). All other chemicals including 97% 1, 1′-diethyl-2, 2′-cyanine iodide (PIC, CAS #:977-96-8); molecular biology-grade water; sodium chloride (NaCl); magnesium chloride (MgCl2); and TRIS (2-Amino-2-(hydroxymethyl)propane-1, 3-diol; pH 7.0) were sourced from Sigma-Aldrich.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!