Collagenase type 4

Manufactured by HiMedia

Sourced in India

Collagenase type 4 is an enzyme used for the dissociation and isolation of cells from various tissues. It is a mixture of proteolytic enzymes that efficiently break down the collagen-rich extracellular matrix, facilitating the release of individual cells.

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Lab products found in correlation

Hematoxylin, by HiMedia (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Gentamycin, by Thermo Fisher Scientific (1 mentions) Transfer buffer, by Genetix (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Ponceau s, by HiMedia (1 mentions) Hanks balanced salt solution (hbss), by HiMedia (1 mentions) Jc 1 assay kit, by Thermo Fisher Scientific (1 mentions) Protein assay kit, by Avantor (1 mentions) Cdna synthesis kit, by Genetix (1 mentions) Ripa lysis buffer, by Avantor (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Merck Group (1 mentions) Sodium cacodylate, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Bcl2 associated x (bax), by Bio-Synthesis (1 mentions) Bcl 2, by Bio-Synthesis (1 mentions) Diethylnitrosamine, by Merck Group (1 mentions)

2 protocols using collagenase type 4

Evaluating Cytotoxicity and Apoptosis in Cancer Cells

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ALA (463-40-1) (0.914gm/ml) was purchased from TCI Chemicals (India) Pvt. Ltd. RPMI 1640 (Gibco, USA); fetal bovine serum (FBS) (Gibco-10270); trypsin (Gibco, USA); eagle balanced salt solution (EBSS)(Gibco, 2018-11); hank’s balanced salt solution (HBSS) (Himedia, TL1190); EtBr (Himedia, MB071); AO(Himedia, MB116); JC-1 assay kit (Thermo Scientific, M34152); PI (SC-3541); tamoxifen citrate (TMX) (Tammodex 20, Biochem Pharmaceuticals India); penicillin- streptomycin (Thermo Scientific, 15410-163); gentamycin (Thermo Scientific, 15710-049);3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) (Himedia, TC191); RNase (SRL, 58895); dimethyl sulfoxide (DMSO) (Merck, 1.16743.0521); DMBA (Sigma Aldrich, 57-97-6); ponceau S (Himedia, ML045); sodium cacodylate (Sigma Aldrich, C0250); collagenase type 4 (Himedia, TC-214); hyaluronidase (Himedia, TC331); hematoxylin (Himedia, S058); eosin (Himedia, S007); RIPA lysis buffer (Amresco, N653); protein assay kit (Amresco, M173); bovine serum albumin (BSA) (Genetix, PG-2330); transfer buffer (Genetix, GX-9411AR), trizol reagent (Sigma-T9424), cDNA synthesis kit (Genetix-K1612). Caspase 3 (SC-4263) and caspase 8 (SC-4267) assay kits were procured from Santacruz Biotechnology Inc., California, Delaware. All others chemicals were of molecular biology grade and purchased from Genetix Biotech Asia Pvt. Ltd, New Delhi.

Roy S., Rawat A.K., Sammi S.R., Devi U., Singh M., Gautam S., Yadav R.K., Rawat J.K., Singh L., Ansari M.N., Saeedan A.S., Pandey R., Kumar D, & Kaithwas G. (2017). Alpha-linolenic acid stabilizes HIF-1 α and downregulates FASN to promote mitochondrial apoptosis for mammary gland chemoprevention. Oncotarget, 8(41), 70049-70071.

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Hepatocellular Carcinoma Induction Protocol

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GLA, diethylnitrosamine (DEN), Eagle balanced salt solution (EBSS), ponceau S, and RNase were procured from Sigma Aldrich (St Louis, MO, USA). Collagenase type 4, RNase, sodium cacodylate, hematoxylin, and eosin were purchased from Himedia. Bax and Bcl-2 were purchased from Biosynthesis Biotechnology (Beijing, China). The kits for aspartate aminotransferase (AST), alanine aminotransferase (ALT), alpha-fetoprotein (AFP), and alkaline phosphatase (ALP) were purchased from Beihuakangtai Biotechnology (Beijing, China). All other chemical and reagents used in the experimental study were acquired from the reputed vendor.

Cui H., Han F., Zhang L., Wang L, & Kumar M. (2018). Gamma linolenic acid regulates PHD2 mediated hypoxia and mitochondrial apoptosis in DEN induced hepatocellular carcinoma. Drug Design, Development and Therapy, 12, 4241-4252.

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