All experimental procedures were approved by the Harvard Medical School Institutional Animal Care and Use Committee and were performed in compliance with the Guide for the Care and Use of Laboratory Animals. All imaging data were obtained from five male C57BL/6J-Tg(Thy1-GCaMP6s) GP4.12Dkim/J mice. For the optogenetic inactivation experiments, three male VGAT-ChR2-YFP mice were used (The Jackson Laboratory). All mice were 8-10 weeks old at the start of behavioral training, 12-24 weeks old during imaging and optogenetic experiments, and had not undergone previous procedures. Mice were kept on a reversed 12 h dark/light cycle and housed in groups of up to four littermates per cage. Mouse health was checked daily.
Vgat chr2 yfp mice
Manufactured by Jackson ImmunoResearch
VGAT-ChR2-YFP mice express channelrhodopsin-2 (ChR2) fused to yellow fluorescent protein (YFP) under the control of the vesicular GABA transporter (VGAT) promoter. This allows for optical control and visualization of GABAergic neurons in these transgenic mice.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using vgat chr2 yfp mice
1
Optogenetic Inactivation in C57BL/6J Mice
2
Optogenetic Inhibition in Mouse Behavior
3
Optogenetic and Calcium Imaging in Murine Cognitive Tasks
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experimental procedures were approved by the Harvard Medical School Institutional Animal Care and Use Committee and were performed in compliance with the Guide for the Care and Use of Laboratory Animals. All optogenetic inhibition data were acquired from 19 male VGAT-ChR2-YFP mice (The Jackson Laboratory, stock 014548). All calcium imaging data were acquired from six C57BL/6J-Tg (Thy1-GCaMP6s) GP4.3Dkim/J mice (stock 024275) of both sexes (five females, one male). Mice were 11–32 weeks old at the start of behavioral training. Age at training start did not vary systematically across tasks (simple task: 21 ± 7 weeks (n = 7), switching task: 17 ± 5 weeks (n = 13), delay task: 21 ± 11 weeks (n = 5), mean ± standard deviation (SD), including photoinhibition and calcium imaging mice). Mice were kept on a reverse dark/light cycle and housed in groups of 2–3 littermates per cage (mice for optogenetic inhibition) or single housed (mice for calcium imaging).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!