As indicated in Supporting Fig. 9 , mice were injected with cardiotoxin at 4 weeks of age, and samples were collected at 17 days postinjury and fixed for 24 hours in 4% paraformaldehyde. HO was detected and quantified in high-resolution, cross-sectional reconstructed images of paraformaldehyde (PFA)-fixed hind limbs using a micro–computed tomography (μCT) VivaCT40 imager (Scanco Medical AG, Brüttisellen, Switzerland) at a source voltage of 55 kV, a source current of 145 μA, and an isotropic voxel size of 19.0 μm. Three-dimensional renderings to quantify HO were reconstructed using Scanco μCT V6.1 software from regions of interest that were free-hand drawn around HO every 5 to 10 reconstructed slices and then interpolated for total volume. Users ensured that the HO region of interest did not include skeletal bone. Thresholding values for HO detection ranged from 240 to 1000 Hounsfield units.
μct v6
Manufactured by Scanco
Sourced in Switzerland
The μCT v6.1 is a high-performance X-ray microtomography system designed for advanced imaging and analysis. It provides detailed, non-destructive 3D visualization and quantification of internal structures within a wide range of samples. The system's core function is to capture high-resolution, high-contrast images that can be used for various analytical applications.
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Lab products found in correlation
6 protocols using μct v6
1
Quantifying Heterotopic Ossification in Mice via Micro-CT Imaging
2
Quantifying Heterotopic Ossification in Mice
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21 days following injury, mice were euthanized and both lower hind limbs were collected and fixed for 2 h in 4% paraformaldehyde at 4 °C. HO was detected and quantified in high‐resolution, cross‐sectional reconstructed images of paraformaldehyde (PFA)‐fixed hind limbs using micro-computed tomography (μCT) VivaCT40 imager (Scanco Medical AG, Brüttisellen, Switzerland) at a source voltage of 55 kV, a source current of 145 µA, and an isotropic voxel size of 19.0 µm. Three‐dimensional renderings to quantify HO were reconstructed using Scanco μCT V6.1 software from regions of interest that were free‐hand drawn around HO every 5–10 reconstructed slices and then interpolated for total volume. Users ensured that the HO region of interest did not include skeletal bone. Thresholding values for HO detection ranged from 240 to 1000 Hounsfield units.
3
Femur Trabecular Bone Microarchitecture Analysis
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After euthanasia, the right femur was dissected from each mouse, fixed for 2 days in 10% neutral buffered formalin, and then transferred into 70% ethanol for μCT scanning on a high-throughput μCT specimen scanner (μCT-35; Scanco Medical AG). The distal 33% of each bone was scanned using the following conditions: 50 kV, 120 mA, 151-ms integration time, 0.5 mm Al filter, and 10-μm voxel resolution (Bouxsein et al., 2010 (link)). Three-dimensional morphometric properties of the distal femur cancellous bone were measured as previously described (Niziolek et al., 2011 (link)). Briefly, trabecular bone volume fraction (BV/TV; %), trabecular number (Tb.N; 1/mm), and trabecular thickness (Tb.Th; mm) were determined on a 1.5 mm region of the distal femur secondary spongiosa, using an ROI beginning 0.6 mm proximal to the distal growth plate (identified by radiolucency and morphology) and extending proximally for 1.5 mm. The trabecular bone was digitally isolated from the cortical compartment by manually lassoing the trabecular bone every 15 slices, then interpolating the trabecular compartment in intervening slices using the contouring function in the Scanco software. All measurements were calculated automatically using the Scanco software (μCT v6.1).
4
Distal Femur Bone Microstructure Analysis
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After euthanasia, the right femur was dissected from each mouse, fixed for 2 d in 10% neutral buffered formalin, and then transferred into 70% ethanol for micro computed tomography (μCT) scanning on a high-throughput μCT specimen scanner (μCT-35; Scanco Medical AG). The distal 33% of each bone was scanned using the following conditions: 50 kV, 120 mA, 151-ms integration time, 0.5 mm Al filter, and 10-μm voxel resolution.29 (link) Three-dimensional morphometric properties of the distal femur cancellous bone were measured as previously described.30 (link) Briefly, trabecular bone volume fraction (BV/TV; %), trabecular number (Tb.N; 1/μm), trabecular thickness (Tb.Th; μm), trabecular spacing (Tb.Sp; μm), and connectivity density (Conn.Dn; 1/μm3) were determined on a 1.5 mm region of the distal femur secondary spongiosa, using an ROI beginning 0.6 mm proximal to the distal growth plate (identified by radiolucency and morphology) and extending proximally for 1.5 mm. The trabecular bone was digitally isolated from the cortical compartment by manually lassoing the trabecular bone every 15 slices, then interpolating the trabecular compartment in intervening slices using the contouring function in the Scanco software. All measurements were calculated automatically using the Scanco software (μCT v6.1).
5
Evaluating Radial Bone Defect Repair
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The defective radius and adjoining ulna were harvested and scanned by a μ-CT imaging system (Scanco medical AG vivaCT40, Switzerland). 3D μ-CT images were reconstructed using the special software μ-CT v6.1 of Scanco medical AG. Slice increment is 19.5 μm. Meanwhile, in order to observe the repair effect directly on the middle of radial bone defects, the middle section images were selected from all the 2D section view images at 2 months with different implants.
6
Quantitative Micro-CT Analysis of Bone and Teeth
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Femora and 5th lumbar vertebrae (LV5) were analyzed by quantitative μCT using an LTC-100 μCT scanner (Hitachi-Aloka, Tokyo, Japan) with an automatic dose setting and with mouse bone mode at 60 μm resolution. Scans of the femora were taken at 0.4 and 4.4 mm from the end of the growth plate for distal metaphysis and midshaft analyses, respectively. Bone mineral content (BMC) and bone area were reported using Aloka software (SYS-C320 version 1.5) as previously described [29 (link)]. Bone mineral density (BMD) was calculated as BMC normalized to bone volumetric area [29 (link)]. μCT measurements of left hemi-mandibles were conducted at Charles River Laboratories Montreal (Senneville, Québec, Canada), with a Scanco μCT 100 scanner (Scanco Medical AG, Brüttisellen, Switzerland) with a dose of 70 kVp, 114 μA, 8 W and at a isotropic voxel resolution of 5 μm. The software used for acquisition was μCT v6.1 (Scanco Medical AG, Brüttisellen, Switzerland) and the software used for analyses was μCT Evaluation v6.5–3 (Scanco Medical AG, Brüttisellen, Switzerland). The evaluation was completed prior to processing portions of the left mandible to ground plastic sections (see above). Evaluation included the determination of enamel (incisor and molar) and pulp volume in a gated area in the left mandible (see S2 Fig and S2 Text for details).
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