Axiovision imager a1

Manufactured by Zeiss

The Axiovision Imager A1 is a compact and versatile microscope imaging system designed for a wide range of applications. It features a high-resolution camera and intuitive software for capturing, processing, and analyzing images. The core function of the Axiovision Imager A1 is to provide users with a reliable and efficient tool for microscopic imaging and analysis.

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Lab products found in correlation

Lsm800 confocal microscope, by Zeiss (4 mentions) Fluorescein labeling mix, by Roche (1 mentions) Goat anti rabbit alexa488 antibody, by Thermo Fisher Scientific (1 mentions) Rabbit anti human γ h2ax, by Cell Signaling Technology (1 mentions) Tricaine, by Merck Group (1 mentions) Acridine orange, by Merck Group (1 mentions)

Close spelling variants of this product

AxioVision A1 Imager A1 AxioVision

5 protocols using axiovision imager a1

Whole-Mount In Situ Hybridization Protocol

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RNA probes were produced with digoxigenin or fluorescein labeling mix (Roche). huc, her4, myoD, dlc, tbx24, her1 and mib plasmids were used as templates26 (link)44 (link)45 (link)46 (link)47 (link). The templates of dystrophin, foxc1a, fgf8a, papc, tcf15 and p53 were generated by RT-PCR with primers (listed in supplementary Table 6). The protocol of WISH followed the instruction of previous literatures48 (link)49 (link)50 (link). Embryos were mounted in 95% glycerol (Sigma, cat:15523)/PBST (UniRegion Bio-Tech, cat:UR-PBS001 and Tween20) pH 5.5 or flat-mounted in 95% glycerol/PBST pH 5.5 or 2:1 benzyl benzoate (Sigma, cat:B6630)/benzyl alcohol (Alfa Aesar, cat:041218). Images were captured by Zeiss Axiovision Imager A1 or Zeiss Discovery V8.

Hsu C.H., Lin J.S., Po Lai K., Li J.W., Chan T.F., You M.S., Tse W.K, & Jiang Y.J. (2015). A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect. Scientific Reports, 5, 10673.

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Quantification of DNA Damage in Zebrafish Embryos

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Laid embryos were collected as mentioned above. The stage of embryos was staged as described42 (link) and doubly checked by WT or WT-like control embryos (the siblings) that were collected in the same time windows. Embryos were dechorionated and sorted into different dishes of mibⁿⁿ²⁰⁰² homozygotes and WT siblings at 14 hpf. WT and mibⁿⁿ²⁰⁰² homozygotes that did not exposed to UV were moved to room temperate for 15 min. At the same time, the WT-UV control was placed in laminar flow hood with UV on for 15 min. Afterwards, all dishes were moved to 28.5 °C incubator for 1 hour before collected. Collected embryos were dehydrated by −20 °C methanol/acetone (1:1) and stored at −20 °C. Embryos were then sorted into 10 embryos/vial and washed by 1% Triton/PBS several times on shaker. After overnight blocking, embryos were washed by 2% BSA/1% Triton/PBS at 4 °C on shaker, and 1:1000 rabbit anti-human γ-H2AX (Cell signaling, #9718) in 2% BSA/1% Triton/PBS was used for overnight reaction. After intensive wash, 1:1000 goat anti-rabbit Alexa-488 antibody (Molecular probe) in 2% BSA/1% Triton/PBS was used for overnight reaction. After intensive wash, embryos were fixed by 4% paraformaldehyde. Embryos were further deyolked and flat-mounted in 75% glycerol to avoid strong background fluorescence from yolk. Images were taken by Zeiss Axiovision Imager A1.

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Quantitative Fluorescence Microscopy Analysis

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For image analysis, at least five sections from each slide (each with different antibodies)/animal were analyzed. Images were captured at the same exposure and threshold, and at the same intensity per condition using Zeiss LSM 800 confocal microscope or Zeiss AxioVision Imager A.1. The automatic cell counter in ImageJ (Rueden et al., 2017 (link); Schneider et al., 2012 (link)) was used to count the total number of cells. Colabeled cells with cell type-specific markers and viral marker RFP were counted manually in separate RGB channels and with the following stereological considerations: 1) systematic and random sampling; 2) calculation of total cell numbers instead of signal densities, 3) counting of cells with aid from DAPI staining, not cell profiles, and 4) specific staining to identify the cells of interest. Data were presented as the mean ± standard error of the mean (SEM). Statistical significance between two conditions was calculated by Student's t-test and multi-group comparisons were performed using one-way ANOVA, followed by Tukey post-hoc test. A p-value of less than 0.05 was considered statistically significant.

Patel M., Anderson J., Lei S., Finkel Z., Rodriguez B., Esteban F., Risman R., Li Y., Lee K.B., Lyu Y.L, & Cai L. (2021). Nkx6.1 enhances neural stem cell activation and attenuates glial scar formation and neuroinflammation in the adult injured spinal cord. Experimental neurology, 345, 113826.

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Apoptosis Assay in Zebrafish Embryos

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Embryos were placed in 2 μg/ml acridine orange (Sigma, cat:A6014)/Egg water for 30 min, then washed 4 times with a 30 min interval. Embryos anaesthetized by tricaine (Sigma, cat:E10521) were mounted in 4% methylcellulose (Sigma, cat:M0387) with E3 egg water on top and captured by Zeiss Axiovision Imager A1 or Zeiss Discovery V8.
Embryos for Tunel assay (Millipore, cat:S7160) were dehydrated in 70% ethanol overnight after fixation. 10 min ethanol/acetic acid treatment at −20 degree was used to increase membrane permeability. After the pretreatment of equilibration buffer at room temperature for 10 min, embryos were placed in TdT labeling solution for 2 hours and then washed by phosphate buffered saline. Embryos were then mounted in 2% low-temperature agarose and captured by Nikon A1 confocal microscope.
The pixels of green fluorescence were counted and assayed by Image J. The statistic calculation of the pixels was carried out by Excel (Microsoft).

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Quantitative Analysis of Cell Types

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At least five sections from each slide/animal were analyzed. Images were captured at the same exposure and threshold, and at the same intensity per condition using a Zeiss LSM 800 confocal microscope or Zeiss AxioVision imager A1. The automatic cell counter in ImageJ⁶⁴ (link) was used to count the total number of cells. Co-labeled cells with cell type-specific markers and viral marker RFP were counted manually using ImageJ in separate RGB channels and with the following stereological considerations: (1) systematic and random sampling; (2) calculation of total cell numbers instead of signal densities; (3) counting of cells, not cell profiles; and (4) specific staining to clearly identify the cells of interest.

Patel M., Li Y., Anderson J., Castro-Pedrido S., Skinner R., Lei S., Finkel Z., Rodriguez B., Esteban F., Lee K.B., Lyu Y.L, & Cai L. (2021). Gsx1 promotes locomotor functional recovery after spinal cord injury. Molecular Therapy, 29(8), 2469-2482.

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