Iron dextran, rabbit polyclonal antibody against 3-nitrotyrosine (anti-3-NT), 2,4-dinitrophenylhydrazine, rabbit polyclonal antibody against dinitrophenol, TIM, triethanolamine hydrochloride, NADH, α-glycerophosphate dehydrogenase, DL-glyceraldehyde 3-phosphate solution, N-ethyl-maleimide, N-acetyl-imidazole and butylated hydroxytoluene were purchased from Sigma (St. Louis, MO, USA). Monoclonal anti-3-NT adducts IgG was obtained from Millipore Corp. (Billerica, MA, USA). Chemiluminescence system was purchased from Pierce (Rockford, USA). Detection kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutathione peroxidase (GPx), glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and nitric oxide were gained from Nanjing Jiancheng Bioengineering Research Institute (Nanjing, China). NAD(H) kits were purchased from Suzhou Comin Biotechnology Co., Ltd. (Suzhou, China). rabbit polyclonal antibody against TIM and protein A/G agarose were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Horseradish peroxidase-conjugated goat anti-rabbit IgG and anti-mouse IgG was obtained from Thermo Fisher (Rockford, USA). All other reagents and chemicals were of analytical grade and purchased from a local reagent retailer.
Triethanolamine hydrochloride
Manufactured by Merck Group
Sourced in United States
Triethanolamine hydrochloride is a chemical compound used as a buffer and pH adjuster in various laboratory applications. It is a colorless, crystalline solid that is soluble in water and other polar solvents. The core function of triethanolamine hydrochloride is to maintain a specific pH range in experimental solutions, buffers, and other lab procedures where pH control is critical.
Automatically generated - may contain errors
Lab products found in correlation
Ampicillin, by Merck Group (2 mentions)
Glycolaldehyde dimer, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (2 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (2 mentions)
Dl dithiothreitol, by Merck Group (2 mentions)
Q sepharose, by GE Healthcare (2 mentions)
Sephacryl s 200, by GE Healthcare (2 mentions)
2 n morpholino ethanesulfonic acid sodium salt, by Merck Group (2 mentions)
Protein a g agarose, by Santa Cruz Biotechnology (1 mentions)
Horseradish peroxidase conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Butylated hydroxytoluene, by Merck Group (1 mentions)
N ethylmaleimide, by Merck Group (1 mentions)
Detection kits for alanine aminotransferase, by Nanjing Jiancheng (1 mentions)
Iron dextran, by Merck Group (1 mentions)
N acetylimidazole, by Merck Group (1 mentions)
α glycerophosphate dehydrogenase, by Merck Group (1 mentions)
Aspartate aminotransferase, by Nanjing Jiancheng (1 mentions)
Glutathione peroxidase, by Nanjing Jiancheng (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
7 protocols using triethanolamine hydrochloride
1
Oxidative Stress Biomarkers Assay
2
Genetic engineering of ∆argF∆argI E. coli
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The ∆argF∆argI double knockout E. coli strain was constructed by the Coli Genetic Stock Center at Yale. ATUM Bio supplied the backbone vector (pD884) for cloning. Terrific Broth (TB), M9 minimal medium, carbenicillin (100 mg/mL), kanamycin (50 mg/mL), and Luria broth (LB)/agar plates containing carbenicillin/kanamycin were obtained from Teknova. Ampicillin, L-rhamnose monohydrate, triethanolamine hydrochloride, sulfuric acid, acetic acid, lithium carbamoyl phosphate dibasic hydrate, L-ornithine monohydrochloride, diacetyl monoxime and L-citrulline were purchased from Sigma Aldrich. Thermo Fisher Scientific provided B-PER Complete Bacterial Protein Extraction Reagent, HisPur Ni–NTA spin plates, antipyrine, SYPRO Orange (5000X) dye, Lipofectamine 2000 and the bicinchoninic acid (BCA) assay kit. Novus Biologicals provided the rabbit polyclonal OTC antibody (NBP1-87408) for western blot analysis. All oligonucleotides in this work were purchased from IDT and Q5 DNA polymerase (NEB) was used for all PCR reactions.
3
Purification and Enzyme Characterization
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Water
was purified using Milli-Q Academic
purification system. Q-Sepharose and Sephacryl S-200 were purchased
from GE Healthcare. Dowex 50WX4-200R (H+ form), nicotinamide
adenine dinucleotide reduced (NADH, disodium salt), dihydroxyacetone
phosphate hemimagnesium salt, glycolaldehyde dimer, 2-(N-morpholino)ethanesulfonic acid sodium salt (MES, ≥99.5%),
triethanolamine hydrochloride (TEA, ≥99.5%), ampicillin, kanamycin
sulfate, andd ,l -dithiothreitol (DTT) were purchased
from Sigma-Aldrich. Protease inhibitor tablets (Complete brand) and
bovine serum albumin, fraction V (BSA), were purchased from Roche.
Ammonium sulfate (enzyme grade), guanidinium hydrochloride (electrophoresis
grade, min. 99%), sodium hydroxide (1.0 N), and hydrochloric acid
(1.0 N) were purchased from Fisher. Sodium phosphite (dibasic, pentahydrate)
was purchased from Fluka, and its water content was reduced to Na2HPO3·0.4H2O as previously described.7 (link) Quikchange II Site-Directed Mutagenesis Kits
were purchased from Agilent Technologies, and λDE3 Lysogenization
Kits were purchased from Novagen. All other chemicals were reagent
grade or better and were used without further purification.
was purified using Milli-Q Academic
purification system. Q-Sepharose and Sephacryl S-200 were purchased
from GE Healthcare. Dowex 50WX4-200R (H+ form), nicotinamide
adenine dinucleotide reduced (NADH, disodium salt), dihydroxyacetone
phosphate hemimagnesium salt, glycolaldehyde dimer, 2-(N-morpholino)ethanesulfonic acid sodium salt (MES, ≥99.5%),
triethanolamine hydrochloride (TEA, ≥99.5%), ampicillin, kanamycin
sulfate, and
from Sigma-Aldrich. Protease inhibitor tablets (Complete brand) and
bovine serum albumin, fraction V (BSA), were purchased from Roche.
Ammonium sulfate (enzyme grade), guanidinium hydrochloride (electrophoresis
grade, min. 99%), sodium hydroxide (1.0 N), and hydrochloric acid
(1.0 N) were purchased from Fisher. Sodium phosphite (dibasic, pentahydrate)
was purchased from Fluka, and its water content was reduced to Na2HPO3·0.4H2O as previously described.7 (link) Quikchange II Site-Directed Mutagenesis Kits
were purchased from Agilent Technologies, and λDE3 Lysogenization
Kits were purchased from Novagen. All other chemicals were reagent
grade or better and were used without further purification.
4
Fatty Acid Metabolism Assay Protocol
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Fatty acid-free bovine serum albumin (FA-free BSA), palmitic acid, triethanolamine hydrochloride (TRA), amyloglucosidase and hexokinase/glucose-6-phosphate dehydrogenase were obtained from Sigma (St. Louis, MO, USA). [1- 14C] palmitic acid was from Perkin Elmer (Woodbridge, ON, Canada). D-[U- 14C] glucose was from GE Healthcare (Mississauga, ON, Canada). Protease (cOmplete Ultra Tablets) and phosphatase (PhosSTOP) inhibitors were from Roche Diagnostics GmbH (Mannheim, Germany). Glucose was measured by the glucose oxidase method using a OneTouch Ultra Mini Monitor. The NEFA kit was from Wako (Mountain View, CA, USA) and the rat insulin ELISA kit was from Alpco (Salem, NH, USA). All antibodies were purchased from Cell Signaling (Danvers, MA, USA) except for SLN which was purchased from Millipore (Billerica, MA, USA).
5
Purification and Characterization of Enzyme
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Water was obtained from
a Milli-Q Academic
purification system. Q-Sepharose and Sephacryl S-200 were purchased
from GE Healthcare. Dowex hydrogen form, nicotinamide adenine dinucleotide,
reduced form (NADH, disodium salt), glycolaldehyde dimer, 2-(N-morpholino)ethanesulfonic acid sodium salt (MES, ≥99.5%),
triethanolamine hydrochloride (≥99.5%), and acetaldehyde were
purchased from Sigma-Aldrich. Ethylammonium chloride,d ,l -dithiothreitol (DTT), sodium hydroxide (1.0 N), and hydrochloric
acid (1.0 N) were purchased from Fisher Scientific. Sodium phosphite
dibasic form was purchased from Riedel de Haën. The λDE3
lysogenization kits were purchased from Novagen. All other chemicals
were reagent grade or better and were used without further purification.
a Milli-Q Academic
purification system. Q-Sepharose and Sephacryl S-200 were purchased
from GE Healthcare. Dowex hydrogen form, nicotinamide adenine dinucleotide,
reduced form (NADH, disodium salt), glycolaldehyde dimer, 2-(N-morpholino)ethanesulfonic acid sodium salt (MES, ≥99.5%),
triethanolamine hydrochloride (≥99.5%), and acetaldehyde were
purchased from Sigma-Aldrich. Ethylammonium chloride,
acid (1.0 N) were purchased from Fisher Scientific. Sodium phosphite
dibasic form was purchased from Riedel de Haën. The λDE3
lysogenization kits were purchased from Novagen. All other chemicals
were reagent grade or better and were used without further purification.
6
Glutathione Reductase Activity Assay
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Acetovanillone ≥ 98% (Item No. W508454), ( ±)-α-lipoic acid synthetic (Item No. T5625 ≥ 99%, powder), OVA-albumin from chicken egg white (Item No. A5503; lyophilized powder, ≥ 98%, grade V), β-NADPH (β-nicotinamide adenine dinucleotide phosphate, Item No. N3886), Na2HPO4 (Item No. 567550), triethanolamine hydrochloride (TEA, Item No. T1502), 5,5′-dithio-bis (2-nitrobenzoic acid, DTNB, Item No. D8130), 2-vinylpyridine (Item No. 132292) and glutathione reductase (GR, Item No. G3664) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Trichloroacetic acid (TCA, Item No. 577970115) and 5-sulfosalicylic acid hydrate (5-SSA, Item No. 575640115) were purchased from Pol-Aura Sp. z o. o. (Lodz, Poland). All other reagents were obtained from R&D Systems Inc. (Minneapolis, MN, USA) and Biorbyt Ltd. (Cambridge, Cambs, UK).
7
Adipogenesis Regulation Protein Analysis
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5,5'dithiobis (2-nitrobenzoic acid), acetyl CoA, DLdithiothreitol (DTT), iodoacetamide, oxaloacetic acid, palmitoyl CoA, phenylmethylsulphonyl fluoride (PMSF) and triethanolamine hydrochloride were all purchased from Sigma-Aldrich (St Louis, MO, USA). Primary antibodies anti-SIRT1, -PGC-1α, -NRF1, -FAS and -CD36 were obtained from Abcam (Cambridge, UK). Primary antibodies anti-FATP1 (SLC27A1), -NRG4 and -EPDR1 were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Primary antibodies anti-p-AMPK (Thr 172), -total AMPK, -Acetylated lysine, -pHSL (Ser 660), -total HSL, -α-tubulin and -histone H3 were obtained from Cell Signalling Technology (Danvers, MA, USA). Primary antibodies anti-UCP1, -SIRT3, -GLUT4 and -ATGL were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
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