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3 protocols using sodium l tartrate

1

Protein Quantification Using Folin-Ciocalteu Assay

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Bovine serum albumin (BSA), glycerol, β‐mercaptoethanol, sodium hydroxide, and sodium L‐tartrate were purchased from Sigma‐Aldrich Co., LLC. (St. Louis, MO). Coomassie Brilliant Blue R‐250 was purchased from Bio‐Rad Laboratories (Hercules, CA). Copper (II) sulfate pentahydrate, 1 N‐hydrochloric acid and 1 N‐sodium hydroxide were purchased from Yakuri Pure Chemicals Co. Ltd. (Kyoto, Japan). Folin‐Ciocalteu's reagent were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). Sodium dodecyl sulfate (SDS) and glycine were purchased from Bio Basic Inc., (Ontario, Canada). Soy bean oil was purchased from Ottogi Co. Ltd. (Seoul, Korea). Trichloroacetic acid was purchased from Kanto Chemical Co. Inc. (Tokyo, Japan). Other reagents used in the experiments were analytical grade.
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2

Enzymatic Assay for Antioxidant and Tyrosinase Inhibition

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Sodium dodecyl sulfate (SDS) and glycine were purchased from Bio Basic Inc., (Ontario, Canada). 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) diammouium salt (ABTS), hippurly‐his‐leu acetate salt (HHL), lung acetone powder from rabbit, mushroom tyrosinase, bovine serum albumin (BSA), casein, hemoglobin, sodium carbonate, sodium hydroxide, sodium L‐tartrate, and potassium hydroxide were purchased from Sigma‐Aldrich Co., LLC. (St. Louis, MO, USA). 3,4‐Dihydroxy‐L‐phenylalanine (L‐DOPA) was purchased from Acros Organics (New Jersey, USA). Copper (II) sulfate pentahydrate was purchased from Yakuri Pure Chemicals Co., Ltd. (Kyoto, Japan). Folin‐Ciocalteu's reagent was purchased Junsei Chemical Co., Ltd. (Tokyo, Japan). Soybean oil was purchased from Ottogi Co., Ltd. (Seoul, Korea). All reagents used analytical grade.
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3

Enzymatic Assays for Biological Activities

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2,2′‐Azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) ammonium salt (ABTS), casein, hemoglobin, hippuryl‐his‐leu acetate salt (HHL), lung acetone powder from rabbit, mushroom tyrosinase, potassium persulfate, and sodium L‐tartrate were purchased from Sigma‐Aldrich. 3,4‐Dihydroxy‐L‐phenylalanine (L‐DOPA) was purchased from Acros Organics. Hydrochloric acid (1 M) and sodium hydroxide (1 M) were purchased from Yakuri Pure Chemicals Co., Ltd.. Folin–Ciocalteu reagent and acetic acid were purchased from Junsei Chemical Co., Ltd. Sodium dodecyl sulfate (SDS) and glycine were purchased from Bio Basic, Inc. Soybean oil was purchased from Ottogi Co., Ltd. All other reagents were of analytical grade.
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