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γ 32p dctp

Manufactured by PerkinElmer
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[γ-32P]-dCTP is a radioactively labeled deoxycytidine triphosphate used in molecular biology applications. It serves as a substrate for DNA synthesis and labeling experiments.

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Lab products found in correlation

Ecorv restriction enzyme, by Thermo Fisher Scientific (1 mentions) High prime dna labeling kit, by Roche (1 mentions) Restriction enzyme, by Thermo Fisher Scientific (1 mentions) 3h serine, by American Radiolabeled Chemicals (1 mentions) Phospholipid standards, by Merck Group (1 mentions)

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32P-dCTP (27 citations)

2 protocols using γ 32p dctp

1

Transgenic Mouse DNA Genotyping

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We performed tail biopsies from three-week-old transgenic mice. The DNA was isolated via the phenol/chloroform extraction method. The DNA was digested with EcoRV restriction enzyme (Fermentas) and separated on a 0.8% agarose gel. Following electrophoresis, the DNA was transferred by capillary blotting onto a GeneScreen hybridization membrane (Perkin Elmer). BC1 transgenic probe 800 bp DNA fragment downstream from an EcoRV site located in the 3′ flank of the BC1 RNA gene was labeled with [γ-32P]-dCTP (PerkinElmer) and high prime DNA labeling Kit (Roche).
Robeck T., Skryabin B.V., Rozhdestvensky T.S., Skryabin A.B, & Brosius J. (2016). BC1 RNA motifs required for dendritic transport in vivo. Scientific Reports, 6, 28300.
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2

Radiolabeled Lipid Biosynthesis Protocol

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Unless otherwise stated, all reagents were of analytical grade and purchased from Sigma‐Aldrich (Buchs, Switzerland) or Merck (Darmstadt, Germany). Restriction enzymes were obtained from Fermentas (St. Leon‐Rot, Germany) and antibiotics from Sigma‐Aldrich, Invivogen (Nunningen, Switzerland) or Invitrogen (Basel, Switzerland). [3H]Serine (1 mCi ml−1, 20 Ci mmol−1) was purchased from American Radiolabeled Chemicals (St. Louis, USA) and [γ‐32P]‐dCTP (3000 Ci mmol−1) from PerkinElmer (Waltham, USA). Phospholipid standards were purchased from Sigma‐Aldrich or Avanti Polar Lipids Inc. (Alabaster, AL).
Farine L., Jelk J., Choi J., Voelker D.R., Nunes J., Smith T.K, & Bütikofer P. (2017). Phosphatidylserine synthase 2 and phosphatidylserine decarboxylase are essential for aminophospholipid synthesis in T rypanosoma brucei. Molecular Microbiology, 104(3), 412-427.
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