Log In Sign up
The largest database of trusted experimental protocols

Amx 400 mhz

Manufactured by Bruker
Visit Supplier
Sourced in United States, Germany

The AMX-400 MHz is a nuclear magnetic resonance (NMR) spectrometer designed for analytical and research applications. It operates at a frequency of 400 MHz and is capable of performing various NMR experiments. The core function of the AMX-400 MHz is to analyze and characterize chemical compounds and materials using NMR spectroscopy.

Automatically generated - may contain errors

Lab products found in correlation

Nicolet 6700, by Thermo Fisher Scientific (3 mentions) Amx 500 mhz spectrometer, by Bruker (1 mentions) Polysep gfc p 4000 column, by Phenomenex (1 mentions) Optilab rex interferometric refractometer, by Wyatt Technology (1 mentions) Dawn heleos, by Wyatt Technology (1 mentions) Agilent 1260, by Agilent Technologies (1 mentions) Chirascan plus, by Applied Photophysics (1 mentions) Avance 3 600 mhz, by Bruker (1 mentions) Silica gel 60, by Merck Group (1 mentions) Cp chirasil dex cb, by Agilent Technologies (1 mentions) Vertex 80v spectrometer, by Bruker (1 mentions) Silica gel g for tlc, by Merck Group (1 mentions) Waters 1525, by Waters Corporation (1 mentions) Waters 2998 pda detector, by Waters Corporation (1 mentions) Avance drx 600, by Bruker (1 mentions) Agilent 6230 tof mass spectrometer, by Agilent Technologies (1 mentions) Pack pro, by YMC (1 mentions) Silica gel plates 60 f254, by Merck Group (1 mentions) Xevo tqd triple quadruple instrument, by Waters Corporation (1 mentions)

12 protocols using amx 400 mhz

1

NMR and GPC-MALLS Analysis of Molecular Weight

Check if the same lab product or an alternative is used in the 5 most similar protocols
The samples for NMR analysis were prepared at 20 mg mL −1 in deuterium oxide and analysed on either an AMX 400 MHz or an AMX 500 MHz spectrometer (Bruker, Rheinstetten, Germany). Molecular weight (Mw) measurements were performed on a GPC-MALLS dRI with a MALLS 120 mW solid-state laser (wavelength, λ658 nm) DAWN® HELEOSTM and Optilab rEX Interferometric Refractometer, respectively (Wyatt Technology Corporation, Santa Barbara, CA, USA). Size exclusion chromatography (SEC) was performed using PolySep™—GFC-P 4000 column (300 × 7.8 mm) protected by a PolySep™ (Phenomenex, Macclesfield, UK) with a GFG-P guard column (35 × 7.8 mm).
Petkova A.I., Kubajewska I., Vaideanu A., Schätzlein A.G, & Uchegbu I.F. (2022). Gene Targeting to the Cerebral Cortex Following Intranasal Administration of Polyplexes. Pharmaceutics, 14(6), 1136.
+ Open protocol
+ Expand
2

Characterization of Natural Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
Optical rotation data were obtained using an Autopol V Plus instrument (Rudolph, Hackettstown, NJ, USA) at 25 °C. IR data were measured on a PE Spectrum RXI spectrophotometer (PerkinElmer) using KBr disks. The UV and ECD spectra were obtained using a Chirascan Plus (Applied Photophysics Ltd., UK). NMR spectra were recorded on Bruker AMX-400 MHz and AMX-600 MHz instruments in CDCl3 with TMS as an internal standard. Mass spectra were obtained on a Dionex Ultimate 3000 UPLC instrument with an LTQ Velos Pro MS spectrometer (Thermo Fisher Scientific, USA). HRESIMS were acquired with a Bruker Daltonics APEXIII 7.0 TESLA FTMS system (Bruker Daltonics, Billerica, MA, USA). Preparative HPLC was performed on an Agilent 1260 (Agilent Technologies) with a JAI C18 column (20 × 240 mm, 5 μm).
Lu Y., Huang Y.S., Chen C.H., Akiyama T., Morris-Natschke S.L., Cheng Y.Y., Chen I.S., Yang S.Z., Chen D.F, & Lee K.H. (2020). Anti-HIV tigliane diterpenoids from Reutealis trisperma. Phytochemistry, 174, 112360.
+ Open protocol
+ Expand
3

Synthesis and Characterization of Thiourethane Silane

Check if the same lab product or an alternative is used in the 5 most similar protocols
Except where noted, all reagents used in this study were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used without further purification. The thiourethane silane was synthesized using trimethylolpropane tris(3-mercaptopropionate) (TMP), 1,3-bis(1-isocyanato-1-methylethyl) benzene (BDI) and 3-(triethoxysilyl)propyl isocyanate, at 2.5:1:1 mol ratios. Reagents were mixed in a 100 mL round-bottom flask containing 50 mL dichloromethane, under magnetic stirring at room temperature, using nitrogen gas as the reaction atmosphere for 30 min. After this time, three drops of triethylamine were added, followed by additional magnetically stirring over an ice bath for 24 h. Reaction completion was confirmed by the disappearance of the isocyanate peak in mid-IR (2270 cm−1). The material was then purified by precipitation in hexanes, and vacuum extraction. The complete consumption of isocyanate was verified by 1H Nuclear Magnetic Resonance — NMR (Bruker AMX-400 MHz, Santa Barbara, CA, USA) using dimethyl sulfoxide-d6 as solvent.
Faria-e-Silva A.L., dos Santos A., Tang A., Girotto E.M, & Pfeifer C.S. (2018). Effect of thiourethane filler surface functionalization on stress, conversion and mechanical properties of restorative dental composites. Dental materials : official publication of the Academy of Dental Materials, 34(9), 1351-1358.
+ Open protocol
+ Expand
4

Analytical Techniques for Secondary Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acetone-d6, chloroform-d, metthanol-d4, and DMSO-d6 were used as the analytical solvent for 1H and 13C NMR spectra (Bruker AMX-400 MHz and a Bruker Avance III-600 MHz). Tetramethylsilane (TMS) was used as a reference to the solvent residue while coupling constants (J) and chemical shifts (δ) are expressed in Hz and parts per million units (ppm) respectively. Silica gel 60 (0.040–0.063 mm, Merck), 60 F254 (Merck), OSD, and Sephadex LH-20 gel were used in different chromatographic techniques (HPLC, column, and TLC). Sulfuric acid 10% and UV light (254 and 365 nm) were used to reveal different secondary metabolites.
Kepdieu Tchebou R.V., Farooq U., Teponno R.B., Wani T.A., Tapondjou L.A., Rasool A., Sarwar R., Khushal A., Bukhari S.M., Zargar S., Xu H.G, & Khan S. (2024). Exploring Cassia mimosoïdes as a promising natural source of steroids with potent anti-cancer, urease inhibition, and antimicrobial properties. RSC Advances, 14(13), 9159-9168.
+ Open protocol
+ Expand
5

Chiral Organic Synthesis via GC

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemical reagents were obtained from commercial sources unless otherwise noted. The 1H and 13C NMR spectra were recorded with a Bruker AMX400 MHz spectrometer using TMS as an internal standard. All compounds were characterized by high resolution mass spectra (HRMS) (Waters GCT PremierTM orthogonal acceleration time-of-flight (oa-TOF) mass spectrometry with an EI Source). GC yields and e.r. values were recorded by SHIMADZU™ GCsolution software version 3 in GC-2014 gas chromatography system with Agilent CP-chirasil-Dex CB (FID, N2 as the carrier gas).
Xu J., Fan J., Lou Y., Xu W., Wang Z., Li D., Zhou H., Lin X, & Wu Q. (2021). Light-driven decarboxylative deuteration enabled by a divergently engineered photodecarboxylase. Nature Communications, 12, 3983.
+ Open protocol
+ Expand
6

Synthesis and Characterization of 4-Isobutylacetophenone

Check if the same lab product or an alternative is used in the 5 most similar protocols
All the chemicals used were of analytical grade and purchased from SD Fine and Himedia. 4-Isobutylacetophenone was obtained from Aldrich Chemical Co. Silica gel-G for TLC (Merck) was used as stationary phase and ethyl acetate : hexane (2 : 8) as mobile phase to check the purity of the compounds. UV light (254 nm) and iodine vapours were used to visualize the spots. Melting points were determined in open capillaries, using Boitus melting point apparatus, expressed in °C, and are uncorrected. The IR spectra were recorded using Bruker Vertex 80v spectrometer. 1H and 13C NMR spectra were recorded on Bruker AMX 400 MHz and chemical shifts are given in units as per million, downfield from tetramethylsilane (TMS) as the internal standard. MS spectra were recorded on Agilent LC-MS spectrometer and elemental analyses were carried out using a Carlo Erba 1108 elemental analyzer for C, H, and N.
Shaik A.B., Yejella R.P, & Shaik S. (2017). Synthesis, Antimicrobial, and Computational Evaluation of Novel Isobutylchalcones as Antimicrobial Agents. International Journal of Medicinal Chemistry, 2017, 6873924.
+ Open protocol
+ Expand
7

Spectroscopic Analysis of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
The NMR experiments were conducted on Bruker Avance DRX-600 and Bruker AMX-400 MHz NMR spectrometers (Bruker BioSpin, Bremen, Germany) in CDCl3 (δH 7.26, δC 77.16). HRESIMS spectra were recorded on an Agilent 6230 TOF mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). MPLC was carried out on an Interchim PuriFlash 450 instrument (Interchim, Montlucon, France). TLC was carried out on silica gel HSGF254 plates (Yantai Jiangyou Silica Gel Limited Company, Yantai, China). Column chromatography was conducted using Sephadex LH-20 (18−110 μm, Pharmacia Co., London, UK) and ODS C18 (15 μm, Santai Technologies, Inc., Changzhou, China). HPLC was performed using a Waters 1525 equipped with a Waters 2998 PDA detector (Waters, Milford, CT, USA). A C18 column (YMC-Pack Pro, 250 × 10 mm, 5 μm, YMC, Kyoto, Japan) was used for RP HPLC.
Wang J., Yan Y.L., Yu X.Y., Pan J.Y., Liu X.L., Hong L.L, & Wang B. (2024). Meroterpenoids from Marine Sponge Hyrtios sp. and Their Anticancer Activity against Human Colorectal Cancer Cells. Marine Drugs, 22(4), 183.
+ Open protocol
+ Expand
8

Characterization of Saccharide Methacrylates

Check if the same lab product or an alternative is used in the 5 most similar protocols
The structures of synthesized saccharide methacrylates were verified by middle infrared spectroscopy, mid-IR, using Nicolet 6700 Thermo Scientific (Waltham, MA, USA) in the range of 4000 at 400 cm−1, with 32 scans at a resolution of 4 cm−1, using NaCl plates. The 1H Nuclear Magnetic Ressonance (1H NMR) spectra (Bruker AMX-400 MHz, Santa Barbara, CA, USA) and the 13C NMR spectra were recorded at 100 MHz, both in DMSO-d6 solvent.
dos Santos A., André C.B., Martim G.C., Schuquel I.T., Pfeifer C.S., Ferracane J.L., Tominaga T.T., Khalil N.M., Radovanovic E, & Girotto E.M. (2018). Methacrylate saccharide-based monomers for dental adhesive systems. International journal of adhesion & adhesives, 87, 1-11.
+ Open protocol
+ Expand
9

Comprehensive Analysis of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents were obtained from
commercial suppliers and used without further purification. Thin-layer
chromatography (TLC) experiments were carried out using precoated
silica gel 60F254. HRMS spectra were recorded on a micromass Q-TOF
spectrometer. 1H and 13C NMR spectra were recorded
on a Bruker AMX 400 MHz and 100 MHz spectrometer, respectively, in
DMSO-d6 and analyzed using MestrNova software
and reported as chemical shifts (δ) in parts per million (ppm).
The coupling constants were reported in hertz (Hz). Column chromatography
for purification of products was performed on silica gel (100–200
mesh). The RP-HPLC experiments were carried out on a Agilent 1260
instrument (chiral column: amylose-2 and cellulose-1, pore size: 5
μm, diameter × length: 4.6 × 250 mm). The melting
points of the compounds were determined on a VEEGO (model: VMP-DS)
melting point apparatus.
Srinivasulu C., Sagar N.R., Vishwanatha T.M., Durgamma S, & Sureshbabu V.V. (2021). Synthesis of Nβ-Protected Amino Sulfenyl Methyl Formamides and Sulfonyl Methyl Formamides: A Simple Protocol. ACS Omega, 6(7), 4680-4686.
+ Open protocol
+ Expand
10

Synthesis and Characterization of Small Hybrid Molecules

Check if the same lab product or an alternative is used in the 5 most similar protocols
All synthetic experiments were performed in an oven-dried apparatus. High resolution mass spectra obtained from a quadrupole/TOF mass spectrometer with an ESI source. Solvents were distilled by standard distillation procedure and stored in 4 Å and 3 Å molecular sieves. 1H (400 MHz), 13C (100 MHz) NMR spectra was recorded with a Bruker AMX-400 MHz instrument. 1H and 13C chemical shifts are referenced to the solvents residual signals D2O 1H NMR δ 4.79, DMSO 1H 2.50 and δ 39.52 for 13C and CD3OD, 1H NMR δ 4.87, 3.31 and 13C δ 49.00 reported in parts per million (ppm) at 25 °C. Coupling constants are expressed in hertz (Hz). Reactions were monitored by thin-layer chromatography carried out on 0.25 mm E. Merck silica gel plates (60F-254), spots were visualized by phosphomolybdic acid and 10% H2SO4 in ethanol. All the reagents used in the preparation of small hybrid molecules were purchased from Sigma-Aldrich and used under good laboratory practice.
Chaurasiya A., Garg S., Khanna A., Narayana C., Dwivedi V.P., Joshi N., e Anam Z., Singh N., Singhal J., Kaushik S., Kaur Kahlon A., Srivastava P., Marothia M., Kumar M., Kumar S., Kumari G., Munjal A., Gupta S., Singh P., Pati S., Das G., Sagar R., Ranganathan A, & Singh S. (2021). Pathogen induced subversion of NAD+ metabolism mediating host cell death: a target for development of chemotherapeutics. Cell Death Discovery, 7, 10.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!