Asiatic acid

Manufactured by Yuanye Bio-Technology

Sourced in China

Asiatic acid is a natural compound derived from various plant sources. It functions as a key active ingredient in pharmaceutical and cosmetic applications. The core function of Asiatic acid is to provide a stable, purified compound for further product development and research purposes.

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3 protocols using asiatic acid

Asiatic Acid Molecular Mechanisms

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Asiatic acid (high-performance liquid chromatography ≥98%; Shanghai Yuanye Biotechnology Co., Ltd.) was dissolved in DMSO. An appropriate concentration of DMSO was used for the experimental controls. Antibodies against Bcl-2 (cat. no. 4223), Bax (cat. no. 2772), phosphorylated (p-)ERK1/2 (cat. no. 4370), ERK1/2 (cat. no. 4695), p38 (cat. no. 8690), p-p38 (cat. no. 4511), JNK (cat. no. 9252), p-JNK (cat. no. 4668), p-PI3K (cat. no. 17366), CDK2 (cat. no. 18048) and cyclin A2 (cat. no. 67955) and the goat anti-rabbit IgG (cat. no. 7074) and anti-mouse IgG (cat. no. 7076) secondary antibodies were purchased from Cell Signaling Technology, Inc. The voltage dependent anion channel 1 (VDAC1; cat. no. ab14734), p-AKT (cat. no. ab192623), AKT (cat. no. ab179463), PI3K (cat. no. ab191606), LC3 (cat. no. ab48394) and p62 (cat. no. ab56416) antibodies were obtained from Abcam. The GAPDH antibody (cat. no. 21612) was purchased from Signalway Antibody LLC.

Pang H., Wu H., Zhan Z., Wu T., Xiang M., Wang Z., Song L, & Wei B. (2023). Exploration of anti‑osteosarcoma activity of asiatic acid based on network pharmacology and in vitro experiments. Oncology Reports, 51(2), 33.

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Extraction and Quantification of Triterpenoids

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Asiatic acid and madecassic acid were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. Absolute ethanol and methanol were purchased from Sinopharm Chemical Reagent Co., Ltd., China. All these reagents are of analytical reagent grade. All of the other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) except where noted. Millipore water (18.2 MΩ·cm) was used in the preparations of all aqueous solutions.

Jiang H., Zheng G., Lv J., Chen H., Lin J., Li Y., Fan G, & Ding X. (2016). Identification of Centella asiatica's Effective Ingredients for Inducing the Neuronal Differentiation. Evidence-based Complementary and Alternative Medicine : eCAM, 2016, 9634750.

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Antioxidant Compounds Characterization

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All chemicals and reagents used in this study were of analytical grade unless stated otherwise. Trolox, gallic acid, Folin–Ciocalteu phenol reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and 2,4,6-tris (2-pyridyl)-s-triazine (TPTZ) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Standard compounds such as hydroxytyrosol, esculin, taxifolin, luteolin, quercetin, kaempferol, apigenin, chlorogenic acid, plantamajoside, rutin, eriodictyol, tiliroside, apigenin-7-O-neohesperidoside, apigenin-7-O-glucoside, luteolin-7-O-glucoside, oleuropein, secoxyloganin, asiatic acid, oleanonic acid, maslinic acid, corosolic acid, oleanolic acid, and ursolic acid were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). For chromatography analysis, HPLC-grade acetonitrile and acetic acid were purchased from Merck (Darmstadt, Germany) and Alfa Aesar (Shanghai, China), respectively. Ultrapure water supplied by a Milli-Q system (Millipore, Bedford, MA, USA) was used throughout the experiments.

Zhang C., Xin X., Zhang J., Zhu S., Niu E., Zhou Z, & Liu D. (2022). Comparative Evaluation of the Phytochemical Profiles and Antioxidant Potentials of Olive Leaves from 32 Cultivars Grown in China. Molecules, 27(4), 1292.

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