Cell light edu apollo 488 in vitro imaging kit 100t

Cells were seeded in 96-well plates at a density of 3 × 10³ cells. Then, cell viability were quantified by CellTiter 96® AQueous One Solution Cell Proliferation Assay (G3580, Promega) with 490 nm plate reading at indicated time points according to the manufacturer's protocol. Cells were seeded into 96-well plates and DNA synthesis was measured using Cell-Light™ EdU Apollo®488 in vitro Imaging Kit (100T) (C10310-3, RiboBio) following the manufacturer's protocols. In brief, cells were labeled with 50 μM EdU for 2 h, then fixed in 4% paraformaldehyde for 30 min, then stained with Apollo®488 and Hoechst 33342. Cells were imaged by Nikon eclipse Ti inverted microscope.

Cell proliferation was measured by the incorporation of 5-ethynyl-2’-deoxyuridine (EdU) during DNA synthesis using the Cell-Light™ EdU Apollo®488 In Vitro Imaging Kit(100 T) (Ribobio, Guangzhou, China) according the manufacture’s instruction. Briefly, the cells were seeded in 96-well plates the day before and incubated with 50 μM EdU for 2 h. Then, the cells were fixed with 4% paraformaldehyde and the cell nuclei were stained with Hoechst. Subsequently, the EdU positive cells were captured and quantified by fluorescence microscopy.