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Staphylococcus aureus s aureus

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Staphylococcus aureus (S. aureus) is a Gram-positive bacterium commonly found in the human respiratory tract and on the skin. It is a well-characterized microorganism used in laboratory settings for various research and testing applications.

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Lab products found in correlation

Escherichia coli e coli, by American Type Culture Collection (10 mentions) Pseudomonas aeruginosa p aeruginosa, by American Type Culture Collection (4 mentions) Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Sodium salt of penicillin g, by Avantor (2 mentions) 2 aminoethyl methacrylate hydrochloride, by Merck Group (2 mentions) Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (2 mentions) Staphylococcus epidermidis, by American Type Culture Collection (1 mentions) S epidermidis, by American Type Culture Collection (1 mentions) Pseudomonas aeruginosa, by American Type Culture Collection (1 mentions) Anhydrous calcium chloride, by Merck Group (1 mentions) Staphylococcus epidermidis s epidermidis, by American Type Culture Collection (1 mentions) Silver nitrate, by Merck Group (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Biowest (1 mentions) Triethanolamine, by ITW Reagents (1 mentions) Triton x 100, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) Polyacrylonitrile pan, by Merck Group (1 mentions) N n dimethylformamide (dmf), by Merck Group (1 mentions) Methacrylic anhydride, by Merck Group (1 mentions)

16 protocols using staphylococcus aureus s aureus

1

Standardized Microbiological Organism Collection

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The following 10 standard organisms Gram-positive, Gram-negative, Microbiology Reference Laboratories were obtained, the American Type Culture Collection (ATCC), 12301 Drive, Rockville, MD 20852, USA: Enterobacter aerogenes (E. aerogenes): ATCC: 13048, Enterococcus faecalis (E.faecalis): ATCC: 29212, Escherichia coli (E.coli): ATCC: 25922, Klebsiella pneumoniae (K. pneumoniae) : ATCC: 700603, Pseudomonas aeruginosa (P.aeruginosa): ATCC: 27853, Serratia marcescens (S.marcescens): ATCC: 8100, Staphylococcus aureus (S.aureus ): ATCC: 29213, Staphylococcus epidermidis (S.epidermidis): ATCC: 12228, Staphylococcus Methicillin Sensitive (MSSA): ATCC: 29213, and Staphylococcus Methicillin-Resistant (MRSA): ATCC: 23591 [42 ].
, & Wadi M. (2022). Bioactivity and Chemical Characterization of Sudanese Bee Honey: Crude Acacia and Its Organic Extracts. BioMed Research International, 2022, 8441239.
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2

Antimicrobial Activity of Ulvan Nanoparticles

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Ulvan was extracted from green algae Ulva Lactuca Linn., ethanol, boric acid, glycerol, deionized water, phosphate-buffered saline (PBS) media, silver nitrate, anhydrous calcium chloride, silver nitrate (AgNO3), Mueller Hinton Agar (MHA) were purchased from Sigma-Aldrich. Star® Ag gel, Escherichia coli (E. coli, ATCC® 35281), Staphylococcus aureus (S. aureus, ATCC® 25923), and Pseudomonas aeruginosa (P. aeruginosa, ATCC® 9027), Staphylococcus epidermidis (S. epidermidis, ATCC® 12228) were purchased from Microbiologics.
Sulastri E., Lesmana R., Zubair M.S., Abdelwahab Mohammed A.F., Elamin K.M, & Wathoni N. (2023). Ulvan/Silver nanoparticle hydrogel films for burn wound dressing. Heliyon, 9(7), e18044.
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3

Antimicrobial Silver Nanoparticle Formulation

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Silver nanoparticles (NTX-300WT) were purchased from Denafu Nanometer Technology Co., Ltd. (Shanghai, China). Propane and butane were obtained from Jiali Daily Chemical Co., Ltd. (Foshan, China). Both poloxamer 407 (WPED612D) and poloxamer 188 (WPOD583B) were purchased from BASF SE (Charlotte, NC). Carbopol (974 P) was obtained from Jiefu Trading Co., Ltd. (Guangzhou, China). Asimi® was purchased from Qinghua Yuanxing Nano-pharmaceutical Co., Ltd. (Shenzhen, China).
Escherichia coli (E. coli, ATCC 25922), Staphylococcus aureus (S. aureus, ATCC 25923), Pseudomonas aeruginosa (P. aeruginosa, ATCC 27853) and Candida albicans (C. albicans, ATCC 64550) were obtained from American Type Culture Collection (ATCC, Manassas, VA). SD rats were provided by Guangdong Medical Laboratory Animal Center (Foshan, China). Studies were conducted in accordance with guidelines and procedures approved by the Institutional Authority for Laboratory Animal Care and Ethics Committee of Sun Yat-sen University.
Mei L., Chen J., Yu S., Huang Y., Xie Y., Wang H., Pan X, & Wu C. (2017). Expansible thermal gelling foam aerosol for vaginal drug delivery. Drug Delivery, 24(1), 1325-1337.
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4

Lavender Oil-based Antimicrobial Formulation

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The lavender essential oil of Lavandula angustifolia was purchased from Naturales Casvior (Bogotá, Colombia). Mineral oil (99%), Tween 20® (Polysorbate 20) (GC grade), Span 80® (Sorbitan Monooleate) (GC grade), Thrombin, and Triton X-100 (laboratory grade) were purchased from Sigma-Aldrich (Milwaukee, WI, USA). Carbopol (poly(acrylic-acid)) (99%), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Triethanolamine (99%) was obtained from PanReac AppliChem (Barcelona, Spain). The Mueller Hinton agar for microbiological testing was acquired from Merl-Millipore (Darmstadt, Germany). The bacteria strains were Staphylococcus aureus (S. aureus) (ATCC 23235) and Escherichia coli (E. coli) (ATCC 25922). The CW49 peptide was purchased from GL Biochem Ltd. (Shanghai, China). The human keratinocytes were HaCaT (CVCL 0038). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Biowest (Kansas City, MO, USA).
Jaramillo V., Díaz E., Muñoz L.N., González-Barrios A.F., Rodríguez-Cortina J., Cruz J.C, & Muñoz-Camargo C. (2023). Enhancing Wound Healing: A Novel Topical Emulsion Combining CW49 Peptide and Lavender Essential Oil for Accelerated Regeneration and Antibacterial Protection. Pharmaceutics, 15(6), 1739.
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5

Antibacterial Polymeric Nanofiber Synthesis

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HNTs were supplied by Esan Eczacıbaşı
(Turkey). ICG and ultrapure Tris Base (Tris-hydroxymethyl aminomethane)
were purchased from MP Biomedicals, LLC. Polyacrylonitrile (PAN) (MW
= 150 kDa) and N,N-dimethylformamide
(DMF) (99.8%) were purchased from Sigma-Aldrich. Tryptic soy broth
(TSB) and agar powder were purchased from Medimark (Italy). Antibacterial
activity tests were carried out using Staphylococcus
aureus (S. aureus) (ATCC 29213). All experiments were performed by using
deionized (DI) water.
Demirel O., Gundogdu S.O., Yuce S, & Unal H. (2023). Indocyanine Green-Loaded Halloysite Nanotubes as Photothermal Agents. ACS Omega, 8(41), 37908-37917.
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6

Antibacterial and Antioxidant Biomaterials

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Chitosan (CTS) with 85% deacetylation (molecular weight < 5 kDa), type B gelatin (GEL), methacrylic anhydride (MA), fluorescamine (98%), dimethyl sulfoxide (DMSO), and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP; 95%) were purchased from Sigma-Aldrich (Aldrich, St. Louis, MO, USA). Gallic acid (GA; 3,4,5-trihydroxybenzoic acid monohydrate, 98%), pyrogallol (PG; 1,2,3-trihydroxybenzene, 99%), and tannic acid (TA, 95%) were obtained from Alfa Aesar (Heysham, UK), and their chemical structures are shown in Figure 1a.
Escherichia coli (E. coli; ATCC 8739), Staphylococcus aureus (S. aureus; ATCC 25923), Porphyromonas gingivalis (P. gingivalis; A7436), Streptococcus mutans (S. mutans; ATCC 700610) strains, and L929 fibroblast cell line (RM60091) were obtained from the Bioresource Collection and Research Center (Hsinchu, Taiwan). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin solution were purchased from Gibco (Langley, OK, USA). The alamarBlue reagent was from Invitrogen (Grand Island, NY, USA). Bacto tryptic soy broth and Wilkins-Chalgren anaerobe broth were purchased from Becton Dickinson (Sparks, MD, USA) and Oxoid (Hampshire, UK), respectively. All reagents were of analytical grade and used as received without further purification.
Chen C.C., Wang J.M., Huang Y.R., Yu Y.H., Wu T.M, & Ding S.J. (2023). Synergistic Effect of Thermoresponsive and Photocuring Methacrylated Chitosan-Based Hybrid Hydrogels for Medical Applications. Pharmaceutics, 15(4), 1090.
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7

Antimicrobial Evaluation of Bacterial Strains

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The Gram-positive bacteria (Bacillus spizizenii (B. spizizenii) ATCC 6633, Staphylococcus aureus (S. aureus) ATCC 6538), and Gram-negative bacteria (Escherichia coli (E. coli) ATCC 8739, Pseudomonas aeruginosa (P. aeruginosa) ATCC 12453, and Proteus mirabilis (P. mirabilis) ATCC 43071) used in this study were obtained from the American Type Culture Collection (ATCC). The bacterial strains were grown at 37 °C and maintained on nutrient agar.
Hamed R., Abu Alata W., Abu-Sini M., Abulebdah D.H., Hammad A.M, & Aburayya R. (2023). Development and Comparative Evaluation of Ciprofloxacin Nanoemulsion-Loaded Bigels Prepared Using Different Ratios of Oleogel to Hydrogels. Gels, 9(7), 592.
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8

Synthesis and Characterization of CoAEMAPF6

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2-Cobaltoceniumamidoethyl methacrylate hexafluorophosphate (CoAEMAPF6) was synthesized according to our earlier reports[52 (link), 56 (link)]. 2-Aminoethyl methacrylate hydrochloride (90%), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC-HCl, 98%), 4-(dimethylamino) pyridine and tetrabutylammonium chloride (TBACl) were purchased from Aldrich and used as received. Water was from Thermo Scientific Nanopure with ion conductivity at 18.2 MΩ. The following bacterial strains were purchased from ATCC: Staphylococcus aureus (S. aureus, ATCC-33591), Bacillus cereus (B. cereus, ATCC 11778), Escherichia coli (E. coli, ATCC-11775), Klebsiella pneumoniae (K. pneumoniae, ATCC-35596), Proteus vulgaris (P. vulgaris ATCC 33420). Nitrocefin was purchased from TOKU-E and used as received. Sodium salt of penicillin-G was purchased from VWR and used as received. 4-Cyanopentanoic acid dithiobenzoate (CPDB) was obtained from Strem Chemical Inc. Azobisisobutyronitrile (AIBN) was recrystallized from methanol before use. All other chemicals and reagents were from commercial sources and used as received.
Pageni P., Yang P., Bam M., Zhu T., Chen Y.P., Decho A.W., Nagarkatti M, & Tang C. (2018). Recyclable Magnetic Nanoparticles Grafted with Antimicrobial Metallopolymer-Antibiotic Bioconjugates. Biomaterials, 178, 363-372.
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9

Synthesis of Cobaltocenium-Containing Monomer

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The monomer 2-Cobaltoceniumamidoethyl methacrylate hexafluorophosphate (CoAEMAPF6) was prepared according to our earlier report.40 2-Aminoethyl methacrylate hydrochloride (90%), 4-(dimethylamino) pyridine tetrabutylammonium chloride (TBACl), and N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC-HCl, 98%) were purchased from Sigma Aldrich and used as received. Tetrakis(hydroxymethyl)phosphonim chloride (referred to hereafter as THPC) was an 80% aqueous solution (practical grade) from Fluka, chloroauric acid (hydrogen tetrachloroaurate(III)) was from Johnson Matthey, assayed at 49.42 %. All the bacteria were obtained from ATCC company: Staphylococcus aureus (S. aureus, ATCC-33591), Escherichia coli (E. coli, ATCC-11775), Klebsiella pneumoniae (K. pneumoniae, ATCC-35596), and Pseudomonas aeruginosa (P. aeruginosa, ATCC-10145). Nitrocefin was purchased from TOKU-E and used as received. Azobisisobutyronitrile (AIBN) was was purchased from VWR and recrystallized from methanol. Sodium salt of penicillin-G was purchased from VWR and 2-Cyano-2-propyl benzodithioate (CPB) was purchased from Strem Chemical Inc. Water was purified using Thermo Scientific Nanopure with ion conductivity at 18.2 MΩ. All other chemicals were from commercial sources and used as received.
Yang P., Pageni P., Rahman M.A., Bam M., Zhu T., Chen Y.P., Nagarkatti M., Decho A.W, & Tang C. (2018). Gold Nanoparticles with Antibiotic-Metallopolymers toward Broad-Spectrum Antibacterial Effect. Advanced healthcare materials, 8(6), e1800854.
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10

Functionalized Nanoparticles for Biomedical Applications

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HNTs were donated by Applied Minerals Inc. (USA). γ-Aminopropyl triethoxysilane (APTES) was purchased from Alfa Aesar (USA). Rifampicin (98%) was purchased from TCI (Japan). Indocyanine green (ICG, 95%) was purchased from Energy Chemical (China). Dimethyl sulfoxide (DMSO) was purchased from Aladdin (China). Lauric acid (97%) and stearic acid (95%) were purchased from Sigma-Aldrich (USA). Sodium alginate (low viscosity) was purchased from J&K (China). CaCl2 (AR, 96.0%) was purchased from Aladdin (China). Cell counting kit-8 (CCK-8) was purchased from WISSEN. Dulbecco's modified eagle medium (DMEM) was purchased from Gibco (USA). 4′,6-Diamidino-2-phenylindole (DAPI) was purchased from Solarbio (China). Alexa Fluor 568-phalloidin and LIVE/DEAD BacLight bacterial viability kit were purchased from Invitrogen (USA). Staphylococcus aureus (S. aureus) was purchased from ATCC (China). Anti-IL-6 antibody (bs-0782R) was purchased from Bioss (China). Anti-CD31 antibody (ab182981), anti-TNF-α antibody (ab212899), and anti-collagen Ⅲ antibody (ab6310) were purchased from Abcam (U.K). Anti-collagen Ⅰ antibody (PA1-26204) was purchased from Invitrogen. All the secondary antibodies were purchased from Servicobio (China).
Ye J.J., Li L.F., Hao R.N., Gong M., Wang T., Song J., Meng Q.H., Zhao N.N., Xu F.J., Lvov Y., Zhang L.Q, & Xue J.J. (2022). Phase-change composite filled natural nanotubes in hydrogel promote wound healing under photothermally triggered drug release. Bioactive Materials, 21, 284-298.
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