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Sybr green qrt pcr reagents

Manufactured by Qiagen
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Sybr green qRT-PCR reagents are a set of laboratory consumables used for quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. The reagents contain Sybr green, a fluorescent dye that binds to double-stranded DNA, enabling real-time monitoring of the PCR amplification process.

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Lab products found in correlation

Enhanced chemiluminescence reagent, by Cytiva (2 mentions) Taqman qrt pcr reagents, by Thermo Fisher Scientific (2 mentions) Microrna 31 mimic, by Thermo Fisher Scientific (2 mentions) Mir 31 antagomir, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (2 mentions) Tnf α, by R&D Systems (2 mentions) Anti h3k4me3 antibody, by Abcam (1 mentions) Anti gapdh, by Abcam (1 mentions) Anti klf4, by Santa Cruz Biotechnology (1 mentions) Anti nestin, by Santa Cruz Biotechnology (1 mentions) Anti lin28, by Santa Cruz Biotechnology (1 mentions) Anti olig2 antibodies, by R&D Systems (1 mentions) Anti sox2, by Santa Cruz Biotechnology (1 mentions) Anti gapdh antibody, by Abcam (1 mentions) Anti oct4, by Santa Cruz Biotechnology (1 mentions) Il 1β, by R&D Systems (1 mentions)

Spelling variants of this product

SYBR Green (372 citations) SYBR green reagent (35 citations) QRT-PCR (10 citations) SYBR Green PCR reagent (7 citations)

2 protocols using sybr green qrt pcr reagents

1

TNF-α-mediated Signaling Pathway Analysis

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TNF-α was from R & D Systems. Secondary antibodies and enhanced chemiluminescence reagents were from Amersham. Anti-TRADD and anti-p65 antibodies were from Santa Cruz Biotechnology. Anti-phosphorylated p65 (p-p65) and anti-AcH3 antibodies were from Cell Signaling, and anti-GAPDH and anti-H3K4Me3 antibodies were from Abcam. Real time qRT primers and Taqman qRT-PCR reagents were from Applied Biosystems. Sybr green qRT-PCR reagents were from Qiagen. MicroRNA-31 mimic, miR-31 antagomiR and negative control (−CT) miR were purchased from Ambion/Life Technologies. MicroRNAs were transfected using Lipofectamine RNAiMax Transfection reagent (Life Technologies).
Rajbhandari R., McFarland B.C., Patel A., Gerigk M., Gray G.K., Fehling S.C., Bredel M., Berbari N.F., Kim H., Marks M.P., Meares G.P., Sinha T., Chuang J., Benveniste E.N, & Nozell S.E. (2015). Loss of tumor suppressive microRNA-31 enhances TRADD/NF-κB signaling in glioblastoma. Oncotarget, 6(19), 17805-17816.
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2

Molecular Profiling of Pluripotent Stem Cells

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IL-1β and TNF-α were from R & D Systems. The secondary peroxidase-conjugated antibodies and enhanced chemiluminescence reagents were purchased from Amersham (Arlington Heights, IL). Anti-KLF4, anti-Lin28, anti-Nestin, anti-Oct4 and anti-SOX2 antibodies were from Santa Cruz. Anti-Olig2 antibodies were purchased from R & D Systems. Anti-GFAP antibodies were from Pharmingen, and anti-Brn2 (POU3F2) and anti-GAPDH antibodies were from Abcam. Taqman qRT-PCR reagents were from Applied Biosystems (Beverly, MA). Sybr green qRT-PCR reagents were from Qiagen (Valencia, CA). All other primers were purchased from Integrated DNA Technologies (Iowa City, IA). MicroRNA-31 mimic, miR-31 AntagomiR and negative control (CT) miR were purchased from Ambion/Life Technologies (Austin, TX). MicroRNAs and shRNA molecules were transfected using Lipofectamine RNAiMax Transfection reagent (Life Technologies, Grand Island, NY). Control and Lin28 specific shRNA were purchased from Dharmacon.
Meares G.P., Rajbhandari R., Gerigk M., Tien C.L., Chang C., Fehling S.C., Rowse A., Mulhern K.C., Nair S., Gray G.K., Berbari N.F., Bredel M., Benveniste E.N, & Nozell S.E. (2018). MicroRNA-31 is Required for Astrocyte Specification. Glia, 66(5), 987-998.
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