The concentration of IgG in the supernatant was measured using an enzyme-linked immunosorbent assay (ELISA) kit in accordance with the manufacturer's protocol (IgG (Total) Human Uncoated ELISA Kit, Invitrogen, USA).
Igg total human uncoated elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The IgG (Total) Human Uncoated ELISA Kit is a quantitative immunoassay designed to measure total IgG levels in human serum or plasma samples. The kit provides the necessary components to perform the enzyme-linked immunosorbent assay (ELISA) procedure.
Automatically generated - may contain errors
Lab products found in correlation
Infinite m200 pro, by Tecan (1 mentions)
Prism software, by GraphPad (1 mentions)
Igg subclass human elisa kit, by Thermo Fisher Scientific (1 mentions)
Biotek gen5, by Agilent Technologies (1 mentions)
Synergy ht, by Agilent Technologies (1 mentions)
Ruxolitinib, by InvivoGen (1 mentions)
Gentamycine, by Thermo Fisher Scientific (1 mentions)
Texmacs medium, by Miltenyi Biotec (1 mentions)
9 protocols using igg total human uncoated elisa kit
1
IgG Quantification using ELISA
2
Quantifying Total Human IgG in Plasma
3
Quantifying Human IgG in Mouse Serum
4
IgG Quantification by ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentration of IgG in the supernatant was
measured using an enzyme-linked immunosorbent assay
(ELISA) kit in accordance with the manufacturer’s
protocol [IgG (Total) Human Uncoated ELISA Kit,
Invitrogen, USA]. Normal serum samples that contained
IgG were used as the positive control.
measured using an enzyme-linked immunosorbent assay
(ELISA) kit in accordance with the manufacturer’s
protocol [IgG (Total) Human Uncoated ELISA Kit,
Invitrogen, USA]. Normal serum samples that contained
IgG were used as the positive control.
5
Quantifying Human IgG in Mouse Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Detection of human IgG in mouse serum samples was performed using the IgG (Total) Human Uncoated ELISA Kit (Invitrogen) according to manufacturer's protocol.
6
Determination of IgG Concentration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentration of IgG in the supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit (IgG (Total) Human Uncoated ELISA Kit, Invitrogen, MA, USA). The test was performed in accordance with the manufacturer's protocol.
7
Trastuzumab Antibody Quantification by ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The supernatants obtained from transfected HEK293T cells were sterile filtered through a 0.22 µm syringe filter. The cleared supernatants from the expressions were used for ELISA analysis. For the detection of trastuzumab antibody the IgG (Total) Human Uncoated ELISA Kit (Thermo Fisher Scientific, 88-50550-88) was used. The ELISA analysis was performed following the manufacturer’s instructions. The samples were prediluted 1:100 in assay Buffer A, followed by 2 serial dilutions of 1:2. Tecan Infinite M200 Pro was used to measure absorbance values at 450 nm and 570 nm. Microsoft Excel files were generated after the measurements, and background-corrected values were calculated according to the manufacturer’s instructions. Then the corrected values were analyzed with 5PL (five parameter logistic) curve mode using online data analysis tool MyAssays (https://www.myassays.com/ ). Mean concentration for a sample in one experiment was calculated using obtained concentration values for serial dilutions. Three independent experiments were performed for all tested samples. The mean concentrations for each sample from each experiment was downloaded into Prism software (GraphPad), and average (mean) values and SD for samples’ concentrations were quantified and applied to generate the corresponding bar plot.
8
Quantitative ELISA for Heat-Exposed Antibodies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ELISA was used to determine the Ab activity after heat exposure.
Depending on the type of Abs, commercially available ELISA kits including
an IgG (Total) human uncoated ELISA kit and an IgG subclass human
ELISA kit (Thermo Fisher, USA) were used. Plates were read at 450
nm on a BioTek Synergy HT plate reader using BioTek Gen5 (BioTek).
Data are representative of an average of three sample replicates and
normalized to the stock or initial Abs activity after vacuum drying.
Depending on the type of Abs, commercially available ELISA kits including
an IgG (Total) human uncoated ELISA kit and an IgG subclass human
ELISA kit (Thermo Fisher, USA) were used. Plates were read at 450
nm on a BioTek Synergy HT plate reader using BioTek Gen5 (BioTek).
Data are representative of an average of three sample replicates and
normalized to the stock or initial Abs activity after vacuum drying.
9
Coculture of Sorted Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sorted Tfh cells, non-Tfh CD4 + T cells and purified CD19 + B cells were cocultured at 50 000 cells/well in 96-well plates (Starstedt) at a 1:1 ratio in 200 µL of TexMACS medium (Miltenyi Biotec) supplemented with gentamycine (Gibco) and 0.1 µg/mL SEB (Toxin Technology, Sarasota, Florida, USA) from 13 patients with SSc (3 dSSc and 10 lSSc) and 6 HC. Supernatants were collected after 6 days of culture. For blocking experiments, 10 µg/mL recombinant Human IL-21 Fc Chimaera Protein (IL-21RFc) (R&D Systems, Lille, France), or 10 µg/mL anti-IL6R-IgG1 (Roactemra, tociluzimab; Roche), or 0.1 µg/mL ruxolitinib (InvivoGen, San Diego, California, USA) were added in the culture. The total IgG and IgM levels were measured in coculture supernatants by ELISA (IgG Total Human uncoated ELISA kit and IgM human uncoated ELISA kit; ThermoFisher) according to the manufacturers' instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!