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Scmos camera

Manufactured by Leica
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The SCMOS camera is a high-performance digital imaging device designed for scientific and industrial applications. It features a scientific CMOS (sCMOS) sensor that delivers fast readout speeds, low noise, and high dynamic range. The camera is capable of capturing detailed images and data for a wide range of applications, including microscopy, spectroscopy, and other scientific research activities.

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Lab products found in correlation

Dm6b microscope, by Leica (2 mentions) Hcx pl apo fluotar 100 1.44 na oil immersion objective, by Leica (2 mentions) Las x premium software, by Leica (2 mentions) Thunder imager 3d assay, by Leica (2 mentions) Masson s trichrome kit, by Abcam (1 mentions) Thunder dmi8, by Leica (1 mentions) Uv 3101pc spectrophotometer, by Shimadzu (1 mentions) Dmi8 microscope, by Leica (1 mentions)

8 protocols using scmos camera

1

Dermis Spheroid Characterization

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Dermis spheroids at different days were fixed in formalin buffered solution (10%) and included in paraffin blocks and 5 μm sections were obtained. Tissue slices were stained with Masson’s trichrome kit (Abcam) following producers’ instructions. Images were acquired in brightfield (40× magnification) by Leica THUNDER DMi8.
Immunofluorescence was performed for Collagen III and CD44 on tissue sections after deparaffination of samples with rabbit polyclonal primary antibody for collagen III (Abcam, 1:200) and mouse monoclonal primary antibody for CD44 (Cell Signalling, 1:200) overnight at 4°C. Alexa 555 donkey anti-rabbit and Alexa 488 goat anti-mouse (ThermoScientific, 1:500 for both) were used as secondary antibody. Nuclei were stained with DAPI Fluoroshield™ solution (Sigma Aldrich, Darmstadt, Germany). Acquisitions were performed by Leica THUNDER DMi8 and images acquired with a Leica sCMOS camera and LAS X 3.0.1 software.
Rescigno F., Ceriotti L, & Meloni M. (2021). Extra Cellular Matrix Deposition and Assembly in Dermis Spheroids. Clinical, Cosmetic and Investigational Dermatology, 14, 935-943.
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2

Tumor Immunophenotyping in C57BL/6J Mice

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C57BL/6J tumor‐bearing mice were dissected at the end of the experiment and tumor tissues were immediately fixed with 4% polyformaldehyde. The process of paraffin embedding and immunohistochemistry against CD8, CD4, and CD45 was conducted by Shanghai ZuoCheng Bio Company (Shanghai, China). Images were acquired by the same company using a Leica DM6 B microscope equipped with an sCMOS camera (Leica, Wetzlar, Germany).
Wang L., Wang P., Chen X., Yang H., Song S., Song Z., Jia L., Chen H., Bao X., Guo N., Huan X., Xi Y., Shen Y., Yang X., Su Y., Sun Y., Gao Y., Chen Y., Ding J., Lang J., Miao Z., Zhang A, & He J. (2023). Thioparib inhibits homologous recombination repair, activates the type I IFN response, and overcomes olaparib resistance. EMBO Molecular Medicine, 15(3), e16235.
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3

Immunohistochemical Staining of XDH

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Immunohistochemistry staining of XDH was performed by Shanghai ZuoCheng Bio Company (Shanghai, China, No. HLug-Ade180Sur-01). Slides were photographed with a LeicDM6 B microscope equipped with sCMOS camera (Leica, Wetzlar, Germany). The staining was evaluated manually and graded using a two-score system based on intensity score and proportion score described previously 28 (link).
Chen M.M., Guo W., Chen S.M., Guo X.Z., Xu L., Ma X.Y., Wang Y.X., Xie C, & Meng L.H. (2023). Xanthine dehydrogenase rewires metabolism and the survival of nutrient deprived lung adenocarcinoma cells by facilitating UPR and autophagic degradation. International Journal of Biological Sciences, 19(3), 772-788.
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4

Characterization of Thin Films

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Absorbance measurements of films
were obtained using a UV-3101PC spectrophotometer (Shimadzu, Columbia,
MD). Fluorescence emission spectra were obtained using a HORIBA Spex
Fluorolog-3-spectrofluorometer (model FL3-22TAU3; Jobin Yvon, Edison,
NJ). All optical measurements were performed with a slit width of
5 nm. Films were mounted onto quartz glass slides for spectrophotometric
measurements. Fluorescence microscopy images were obtained using a
Leica DM6B upright microscope equipped with a Hamamatsu sCMOS camera,
Leica DFC450 color CCD, and Spectra X LED light engine and employing
green, blue, and bright field filters.
Ayala C.E., Pérez R.L., Mathaga J.K., Watson A., Evans T, & Warner I.M. (2022). Fluorescent Ionic Probe for Determination of Mechanical Properties of Healed Poly(ethylene-co-methacrylic acid) Ionomer Films. ACS Applied Polymer Materials, 4(2), 832-841.
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5

C. elegans Male Formation and Imaging

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Wild type and akir-1(gk528) males were imaged at 100x on DIC while immobilized with beads and an agar pad. Images were taken with an Andor Zyla sCMOS camera on a Leica DMi8 microscope. Males were obtained from populations of mating isogenic strains. C. elegans males are formed by random non disjunction of the X-chromosome (which can be facilitated by 4-hour incubation at 32°). Once a male (X0) was isolated it was crossed to hermaphrodites (XX) of the same genotype. 50% of crossed progeny were males. Mating plates were maintained for the duration of the experiments from which males were collected.
Bowman R., Balukoff N., Clemons A., Koury E., Ford T., Baxi K., Egydio de Carvalho C, & Smolikove S. (2019). Akirin Is Required for Muscle Function and Acts Through the TGF-β Sma/Mab Signaling Pathway in Caenorhabditis elegans Development. G3: Genes|Genomes|Genetics, 10(1), 387-400.
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6

Fluorescent In Situ Hybridization of Yeast Cells

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Cells were grown to mid-logarithmic phase and fixed with 4% formaldehyde in 0.1 M potassium phosphate buffer. Cells were then converted to spheroplasts using 0.1 M potassium phosphate buffer containing 1.2 M sorbitol and 500 μg of zymolyase. Spheroplasts were washed in 2× SSC buffer and incubated overnight at 37 °C with Cy3-labeled oligonucleotide probe (5′-Cy3-ATG CTC TTG CCA AAA CAA AAA AAT CCA TTT TCA AAA TTA TTA AAT TTC TT-3′) that is complementary to the 5′ portion of ITS1 (Faza et al., 2012). DNA was stained with 0.5 μg/ml DAPI. Cells were visualized using THUNDER Imager 3D Assay (Leica, Germany) equipped with a HCX PL-APO Fluotar 100×/1.44 NA oil immersion objective (Leica, Germany). Images were acquired with a fitted digital sCMOS camera and LAS X premium software (Leica, Germany).
Viéitez C., Busby B.P., Ochoa D., Mateus A., Memon D., Galardini M., Yildiz U., Trovato M., Jawed A., Geiger A.G., Oborská-Oplová M., Potel C.M., Vonesch S.C., Tu C.S., Shahraz M., Stein F., Steinmetz L.M., Panse V.G., Noh K.M., Savitski M.M., Typas A, & Beltrao P. (2021). High-throughput functional characterization of protein phosphorylation sites in yeast. Nature biotechnology, 40(3), 382-390.
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7

Immunohistochemical Analysis of Tumor Tissues

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Tumor tissues were fixed in 4% paraformaldehyde. Paraffin embedding, H&E staining and immunohistochemistry against CD45, CD4, CD8, CD11b, F4/80, CD206, cleaved-caspase 3, and Ki67 were conducted by Shanghai ZuoCheng Bio Company (Shanghai, China). Slides were observed under a Leica DM6 B microscope equipped with sCMOS camera (Leica, Wetzlar, Germany).
Sun P., Zhang X., Wang R.J., Ma Q.Y., Xu L., Wang Y., Liao H.P., Wang H.L., Hu L.D., Kong X., Ding J, & Meng L.H. (2021). PI3Kα inhibitor CYH33 triggers antitumor immunity in murine breast cancer by activating CD8+T cells and promoting fatty acid metabolism. Journal for Immunotherapy of Cancer, 9(8), e003093.
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8

Fluorescent In Situ Hybridization of Yeast Cells

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Cells were grown to mid-logarithmic phase and fixed with 4% formaldehyde in 0.1 M potassium phosphate buffer. Cells were then converted to spheroplasts using 0.1 M potassium phosphate buffer containing 1.2 M sorbitol and 500 μg of zymolyase. Spheroplasts were washed in 2× SSC buffer and incubated overnight at 37 °C with Cy3-labeled oligonucleotide probe (5′-Cy3-ATG CTC TTG CCA AAA CAA AAA AAT CCA TTT TCA AAA TTA TTA AAT TTC TT-3′) that is complementary to the 5′ portion of ITS1 (Faza et al., 2012). DNA was stained with 0.5 μg/ml DAPI. Cells were visualized using THUNDER Imager 3D Assay (Leica, Germany) equipped with a HCX PL-APO Fluotar 100×/1.44 NA oil immersion objective (Leica, Germany). Images were acquired with a fitted digital sCMOS camera and LAS X premium software (Leica, Germany).
Viéitez C., Busby B.P., Ochoa D., Mateus A., Memon D., Galardini M., Yildiz U., Trovato M., Jawed A., Geiger A.G., Oborská-Oplová M., Potel C.M., Vonesch S.C., Tu C.S., Shahraz M., Stein F., Steinmetz L.M., Panse V.G., Noh K.M., Savitski M.M., Typas A, & Beltrao P. (2021). High-throughput functional characterization of protein phosphorylation sites in yeast. Nature biotechnology, 40(3), 382-390.
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