Human cd11c

Formalin-fixed paraffin-embedded (FFPE) blocks of GD thyroid tissues (n=4) were cut into 4-μm serial sections for immunohistochemical staining. Immunostaining for human CD11c (at a 1:300 dilution, Abcam, Cambridge, UK), human CD19 (at a 1:400 dilution, Abcam, Cambridge, UK), or CXCL10 (at a 1:200 dilution, Proteintech, China) was performed by 3,3’-diaminobenzidine (DAB) staining (Zhongshan Jinqiao Biotechnology, Beijing, China) according to the manufacturer’s instructions. CD19 antigen repair was conducted using citric acid solution (pH=6.0) for 15 min by microwave heating. CD11c and CXCL10 antigen repair was performed with Tris–EDTA buffer and heated for 15 min in a microwave. Two sections were prepared as negative controls for each immunostaining batch.

PCL (Mw = 80 kDa) was purchased from Sigma-Aldrich (St. Louis, MO, United States). Dichloromethane (DCM) and N, N-dimethylformamide (DMF) were purchased from BDH Chemicals (Dawsonville, GA, United States). DAPI was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, United States). Antibodies, including human CD8 (1:250), human CD4 (1:500), human CD3 (1:500), human CD11c (1:300) were ordered from Abcam (Cambridge, MA, United States). GM-CSF recombinant human protein and GM-CSF human ELISA kit were purchased from Invitrogen (Carlsbad, CA, United States).