Surgery was conducted under isoflurane anesthesia, following the baseline VEP recording. Ophthalmic proparacaine (Akorn, Inc) was applied topically, and the edges of the upper and lower eyelids trimmed. The lids were stitched together using 7–0 prolene suture (Ethicon, Inc), and Gluture tissue glue (Abbott Laboratories) sealed the lids together. A thin film of cyanoacrylate (tissue glue) was used to cover the area to protect the surgical site during the 7-d period of MD.
7 0 prolene suture
Manufactured by Johnson & Johnson
Sourced in United States
The 7–0 prolene suture is a sterile, synthetic, nonabsorbable monofilament suture material made of polypropylene. It is designed for use in delicate ophthalmic and other fine surgeries.
Automatically generated - may contain errors
Lab products found in correlation
2 2 2 tribromoethanol, by Merck Group (2 mentions)
4 0 vicryl suture, by Johnson & Johnson (2 mentions)
5 0 vicryl suture, by Johnson & Johnson (1 mentions)
Rnu nude rats, by Charles River Laboratories (1 mentions)
Somnosuite low flow anesthesia system, by Kent Scientific (1 mentions)
Inveon, by Siemens (1 mentions)
6 0 prolene suture, by Johnson & Johnson (1 mentions)
Octopus stabilizer, by Medtronic (1 mentions)
18 protocols using 7 0 prolene suture
1
Eyelid Suturing and Tissue Gluing for Visual Deprivation
2
Murine Myocardial Infarction Model
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In total, the left anterior descending coronary artery (LAD) of 10-week-old male C57BL/6 mice (22–25 g) was ligated to establish MI model as described previously20 (link),21 (link). Briefly, mice were anesthetized with injected intraperitoneally with 2,2,2-tribromoethanol (200 mg/kg; Sigma, St. Louis, MO, USA), and then intubated to rodent ventilator (Shinano, Tokyo, Japan). Left thoracotomy was performed with a small incision at the third and fourth intercostal space, and 7-0 prolene suture (Ethicon, Inc., Somerville, NJ, USA) was applied to ligate LAD. The sham-operated mice for control underwent similar processes without tying the suture. The mice were sacrificed by cervical dislocation at 12 h after MI, randomization and blinding were adopted in animal experiments. All operations were performed under sterile conditions.
3
Sheep Carotid Artery Vascular Graft Implantation
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The Animal Care and Use Committee at Q-Test Laboratories (Columbus, Ohio) approved the care, use, and monitoring of animals for sheep experiments. Four custom-made nanofiber TEVGs were implanted bilaterally as AV shunts between the common carotid artery (CCA) to the ipsilateral external jugular vein (EJV) in four sheep. Implantation was accomplished as previously described.16 (link) Briefly, all sheep were anesthetized with 1% to 2% isoflurane and positioned in the dorsal recumbency during surgery. Heparin (100 IU/kg) was administrated intravenously after exposure of the bilateral CCA and EJV. Standard vascular anastomosis was performed with a 7-0 Prolene suture (Ethicon Inc, Somerville, NJ). Hemostasis was obtained, and the muscle, subcutaneous tissue, and dermal incision layers were closed. Antibiotic treatment (cefazolin) was administrated intraoperatively and for 7 days postoperative. All sheep were maintained on a daily oral medication of aspirin (325 mg/d) until the end of the study. Serial color Doppler ultrasound examinations were performed to estimate graft patency and to measure the lumen diameter and the blood flow velocity. Animals were humanely killed by pentobarbital sodium one month after implantation.
4
Inferior Vena Cava Ligation for Venous Thrombosis
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VT was formed via generation of stasis blood flow by infrarenal inferior vena cava (IVC) ligation.19 (link), 20 (link), 21 (link), 22 (link) In brief, the mice were anesthetized via 2% to 2.5% inhaled isoflurane with oxygen gas at 0.5 L/min, and midline laparotomy was performed. The venous side and dorsal branches were interrupted, and the infrarenal IVC was ligated with a 7-0 Prolene suture (Ethicon Inc, Somerville, NJ) to generate stasis thrombosis. The peritoneum was closed with 5-0 Vicryl suture (Ethicon Inc), and the skin incision was secured with skin glue or wound clips (7-mm wound clips; Reflex Inc, San Francisco, CA), and the mice were allowed to recover under a warming lamp. The mice were euthanized on postoperative day 21. Before processing, the IVC and thrombus were measured and weighed en bloc.18 (link), 19 (link), 20 (link)
5
Murine Myocardial Infarction Model
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The male C57BL/6 mice (about 8 weeks, 22–25 g) were housed at 23 ± 2°C under a cycle of 12/12 h light/ dark condition with free access to food and water. The AMI model was established as described previously (14 (link)). The 2,2,2-tribromoethanol (200 mg/kg; Sigma, St. Louis, MO, USA) was intraperitoneally injected to anesthetize animals. In the AMI group, a left thoracotomy was performed with a small incision at the third and fourth intercostal space, and a 7-0 prolene suture (Ethicon, Inc., Somerville, NJ, USA) was applied to ligate the left anterior descending branch (LAD). Mice in the sham group underwent the same procedure without stitches. The mice were sacrificed by cervical dislocation at 12 h after myocardial infarction (MI). All operations were performed under aseptic conditions. The procedures for experiments and animal care were approved by the West China Hospital (Approval Number: 2020020A) and conformed to the Guide for the Care and Use of Laboratory Animals produced by the National Institutes of Health.
6
Rat Myocardial Infarction Model
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Following procedures we have described previously (67 ), male athymic rats (8 to 10 weeks old, RNU Nude rats, Charles River) were anesthetized with isoflurane, intubated, and mechanically ventilated (SomnoSuite Low-Flow Anesthesia System, Kent Scientific). Rodent Surgical Monitor (INDUS instruments) was used to monitor animal’s condition throughout the surgery, including heart rate, body temperature, and heart function through ECG. A left thoracotomy was performed to expose the heart. The pericardium was opened, and the left anterior descending coronary artery was ligated below the left atrial appendage level using a 7-0 Prolene suture (Ethicon). Occlusion was confirmed by LV blanching and ST elevation on ECG after ligation. After 1 hour, the ligation was released, the chest cavity was aseptically closed, and the rat was allowed to recover for 1 day for echocardiographic measurement. For short-term histological analysis (time points up to and including day 7), rats with I/R injuries that caused a measurable decrease in FS were deemed sufficient for injection. For long-term histological analysis (day 28) and functional recovery studies, the rats that met the echocardiographic inclusion criterion (FS < 30%) were selected for experiments and randomly assigned into experimental groups.
7
Murine IVC Thrombosis Model
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VT was created by inducing complete blood stasis via infrarenal IVC ligation as previously described9 (link),15 (link),16 (link). Briefly, mice were initially sedated in an induction chamber with 4% isoflurane and oxygen, and then anesthetized by 2% inhaled isoflurane via nose cone. A midline laparotomy was performed, viscera were rotated laterally to expose the IVC and the venous side branches and dorsal branches were cauterized or ligated. The infrarenal IVC was ligated with a 7–0 prolene suture (Ethicon Inc., Somerville NJ) to generate stasis thrombosis. IVC, thrombi and plasma were harvested at 4, 8 and 21 days following IVC ligation22 (link). The IVC and thrombus of each mouse was first measured (cm) and weighed (grams) to quantify size. The specimens were then separated and snap frozen in liquid nitrogen. At the final time point (21 days), the vein wall and thrombus are inseparable due to fibrotic changes, and hence, the tissue must be analyzed together.
8
Cardiac Injury Models in Zebrafish and Mice
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Cardiac injuries were carried out in 4–12-month old zebrafish6 (link)–8 (link). Briefly, cryoinjury was performed by application of a cryoprobe frozen with liquid nitrogen to the surface of the exposed ventricle until the probe was fully thawed, damaging approximately 20% of the ventricle. For ventricle resection, 20% of the apex of the ventricle was surgically removed, resulting in the immediate formation of a blood clot. Exposing the ventricle, without injury, was performed for sham controls.
Permanent ligation of the left anterior descending coronary artery to induce MI in the adult mouse was performed56 (link). For neonatal MI, mice were anaesthetized using isoflurane 2% and then cooled on ice to induce cardio-respiratory arrest57 (link). A lateral thoracotomy was then performed, and a 7-0 prolene suture (Ethicon) then tied around the left anterior descending coronary artery to induce MI. The chest and skin were closed with 7-0 prolene, and pups were re-warmed under a heat lamp before being returned to the mother.
Permanent ligation of the left anterior descending coronary artery to induce MI in the adult mouse was performed56 (link). For neonatal MI, mice were anaesthetized using isoflurane 2% and then cooled on ice to induce cardio-respiratory arrest57 (link). A lateral thoracotomy was then performed, and a 7-0 prolene suture (Ethicon) then tied around the left anterior descending coronary artery to induce MI. The chest and skin were closed with 7-0 prolene, and pups were re-warmed under a heat lamp before being returned to the mother.
9
Myocardial Infarction and Stem Cell Therapy
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Myocardial infarction was simulated through surgical ligation of the left anterior descending (LAD) coronary artery with a 7/0 prolene suture (Ethicon, Inc., Somerville, NJ, USA) and successful ligation was verified through observation of a color change with the naked eye from red to white in the infarct area. 30 wild-type specific pathogen free C57/BL6 mice (8–10 weeks) were randomly assigned to three groups: CSC, bFGF-treated CSC (45 ng/ml) and bFGF-treated CSC + Akt inhibitor (5.0 mg/kg oral Akt inhibitor for 3 days prior to transplantation). For cell transplantation, animals received intra-myocardium injections of 1×106 cell solution immediately following LAD ligation. All animals were sacrificed one week following cell transplantation. A further 6 mice underwent LAD ligation without cell transplantation. bFGF protein expression and Sca-1+ cell aggregation was measured in these mice one week following simulated myocardial infarction.
10
IVC Ligation Thrombosis Model in Mice
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