Ivis lumina lt

Manufactured by PerkinElmer

Sourced in United States, Germany

The IVIS Lumina LT is a bioluminescence and fluorescence imaging system designed for in vivo and ex vivo applications. It features a sensitive CCD camera, motorized stage, and a range of filter sets for detecting various reporter genes and fluorescent probes.

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Lab products found in correlation

Living image software, by PerkinElmer (8 mentions) D luciferin, by Promega (3 mentions) D luciferin, by Yeasen (2 mentions) Living image 4, by PerkinElmer (2 mentions) Tm300, by Courage + Khazaka (1 mentions) Fox chase scid beige mice, by Charles River Laboratories (1 mentions) Vivoglo, by Promega (1 mentions) Photon imager, by Biospace (1 mentions) Beadblaster 24 homogenizer, by Benchmark Scientific (1 mentions) D luciferin, by Ozyme (1 mentions) Isoflurane, by Fujifilm (1 mentions) Pgl4.51 luc2 cmv neo vector, by Promega (1 mentions) Growth factor reduced matrigel, by BD (1 mentions) Living image v 4, by PerkinElmer (1 mentions) Balb c nu mice, by Charles River Laboratories (1 mentions) D luciferin, by Fujifilm (1 mentions) All in one microscope, by Keyence (1 mentions) Bz 2 analyzer, by Keyence (1 mentions) Female c57bl 6 mice, by Charles River Laboratories (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions)

57 protocols using ivis lumina lt

Bioluminescent Tumor Imaging in Mice

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The mice transplanted CDS tumor cells introduced with the luciferase cDNA were administrated with luciferin. Illumination from the labeled tumor cells were monitored by the IVIS Lumina LT imaging system (Perkin Elmer).

Yoshimoto T., Tanaka M., Homme M., Yamazaki Y., Takazawa Y., Antonescu C.R, & Nakamura T. (2017). CIC-DUX4 induces small round cell sarcomas distinct from Ewing sarcoma. Cancer research, 77(11), 2927-2937.

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Topical Nanogel Therapy for Psoriasis-like Skin Inflammation in Mice

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The total number of mice used in the study was 24 (6 animals for each treatment groups). The Balb/c mouse back was topically treated with imiquimod (IMQ) cream (Aldara, Inova) at 62.5 mg for 5 days to evoke psoriasis-like lesions (van der Fits et al., 2009 (link)). Before IMQ activation, nanogels (100 μL) containing 62.5 μg/mL AIPH and 25 μg/mL HPTS with or without heating were applied to the dorsal skin. The phenotypic skin surface was monitored by a digital camera and hand-held microscopy (M&T Optics). The combined score (erythema, scaling, and skin thickness) was estimated to reveal the inflammation severity (scale 0–12) according to the Psoriasis Area and Severity Index (PASI) scoring. The erythema (a*) and transdermal water loss (TEWL) of the skin were estimated by colorimetry (Yokogawa CD100) and a Tewameter (Courage & Khazaka TM300). An in vivo imaging system (IVIS) was used to visualize the cutaneous fluorescence derived from the topically applied nanogels. The nanocarriers were administered to the skin each day for 5 days. The IVIS (PerkinElmer IVIS Lumina LT) was used to image the dorsal skin 24 h postadministration. The excitation and emission wavelengths were set at 430 nm and 445–490 nm, respectively, for IVIS to image the HPTS fluorescence.

Nirmal G.R., Liao C.C., Lin Z.C., Alshetaili A., Hwang E., Yang S.C, & Fang J.Y. (2023). Topically applied pH-responsive nanogels for alkyl radical-based therapy against psoriasiform hyperplasia. Drug Delivery, 30(1), 2245169.

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Murine Multiple Myeloma Disease Model

Cited 1 time

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All experimental studies and procedures involving mice were performed in accordance with approved protocols from the Garvan Institute/St. Vincent’s Hospital Animal Ethics committee ARA 14/03 (Sydney, Australia) and the Maine Medical Center Research Institute (Scarborough, ME, USA) Institutional Animal Care and Use Committee (IACUC). All mice were weaned at 21 days after birth and fed a diet of normal chow and autoclaved water. Six-week old C57BL/KaLwRiJHsd (BKAL) male mice were injected with vehicle or 5TGM1 cells as described previously (2 (link)). In the MM.1S study, 12-week old female Fox Chase SCID-Beige mice (Charles River Laboratory; Wilmington, MA, USA) (n=3–6) were inoculated via tail vein injections with 5×10⁶ MM.1S^gfp+luc+ cells. “Survival endpoints” were euthanasia following MM-induced hind limb paralysis; mice from a predetermined endpoint (21 days following tumor cell inoculation) were used for measuring whole marrow gene expression (n=6). Tumor burden was measured in the MM.1S studies weekly or biweekly utilizing in vivo bioluminescence imaging in an IVIS Lumina LT (Perkin Elmer; Waltham, MA) as described previously (18 ). Following sacrifice, mouse tibia and femora were fixed and stained with hematoxylin and eosin prior to sectioning and imaging as previously described (19 (link)).

Fairfield H., Dudakovic A., Khatib C.M., Farrell M., Costa S., Falank C., Hinge M., Murphy C.S., DeMambro V., Pettitt J.A., Lary C.W., Driscoll H.E., McDonald M.M., Kassem M., Rosen C., Andersen T.L., van Wijnen A.J., Jafari A, & Reagan M.R. (2020). Myeloma-modified adipocytes exhibit metabolic dysfunction and a senescence-associated secretory phenotype (SASP). Cancer research, 81(3), 634-647.

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Bioluminescence Imaging for Tumor Burden

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Starting on day 7, and biweekly until day 30, tumor burden was assessed with bioluminescence imaging (BLI) in an IVIS^® Lumina LT (Perkin Elmer Inc.; Waltham, MA, USA) equipped with a CCD camera (cooled at −90°C), mounted on a light-tight specimen chamber. Mice were injected with 150 mg/kg i.p. filter-sterilized D-luciferin substrate (VivoGlo, Promega; Madison, WI, USA) and imaged after 10 minutes. Data were acquired and analyzed using LivingImage software 4.5.1. (PerkinElmer). BLI flux equaling the radiance (photons/s) in each pixel integrated over the region of interest (ROI) area (cm²), where the ROI was the whole mouse, was used to quantify tumor burden. BLI and mouse weight data were graphed and analyzed only for days in which all mice remained in the study to avoid artifacts due to mouse death.

Natoni A., Farrell M.L., Harris S., Falank C., Kirkham-McCarthy L., Macauley M.S., Reagan M.R, & O’Dwyer M. (2020). Sialyltransferase inhibition leads to inhibition of tumor cell interactions with E-selectin, VCAM1, and MADCAM1, and improves survival in a human multiple myeloma mouse model. Haematologica, 105(2), 457-467.

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Tracking Exosomes Delivery to Flaps

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To confirm the delivery and uptake of BMSCs-exosomes by flaps after IR injury, exosomes were labeled with DiR Iodide (DiIC18(7), 40757ES25, YEASEN Biotech Co., Ltd., Shanghai, China) and injected through the tail vein into experimental animals. Exo-red fluorescence in animals was detected using a Pre-clinical In Vivo Imaging System (IVIS Lumina LT, PerkinElmer, Waltham, MA, USA) at 0.5, 1, 3, and 6 h after reperfusion.

Niu Q., Yang Y., Li D., Guo W., Wang C., Xu H., Feng Z, & Han Z. (2022). Exosomes Derived from Bone Marrow Mesenchymal Stem Cells Alleviate Ischemia-Reperfusion Injury and Promote Survival of Skin Flaps in Rats. Life, 12(10), 1567.

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Luciferase Complementation Imaging of RIPK-RIN4 Interaction

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For luciferase complementation imaging assays (LCI), the full-length coding sequence of ScRIPK/AtRIPK was cloned into Nluc, and the full-length coding sequence of ScRIN4/AtRIN4 was cloned into Cluc. The Nluc-ScRIPK, Cluc-ScRIN4, Nluc-AtRIPK, Cluc-AtRIN4, and the empty vectors Nluc and Cluc were transformed into Agrobacterium strain GV3101, respectively. Then, Nluc-ScRIPK was mixed with Cluc-ScRIN4 for the transient infiltration of N. benthamiana leaves. The same was done for Nluc-AtRIPK and Cluc-AtRIN4 as the positive control. Nluc-ScRIPK + Cluc, Nluc + Cluc-ScRIN4, Nluc-AtRIPK+ Cluc, Nluc + Cluc-AtRIN4, and Nluc + Cluc were used as the controls. After 2 days, LUC activity was observed and imaged using a CDD imaging apparatus (IVIS Lumina LT, PerkinElmer, USA).

Fang J., Chai Z., Huang R., Huang C., Ming Z., Chen B., Yao W, & Zhang M. (2023). Receptor-like cytoplasmic kinase ScRIPK in sugarcane regulates disease resistance and drought tolerance in Arabidopsis. Frontiers in Plant Science, 14, 1191449.

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ICD@PsPC NPs Biodistribution Imaging

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When the tumor volume reached ~400 mm³, mice were given a tail vein injection of ICD@PsPC NPs. The fluorescence intensities were photographically recorded by IVIS Lumina LT (PerkinElmer, Waltham, MA, USA) at 0, 0.5, 1, 2, 4, and 6 h after injection.

Zhang C., Zhong W., Cao Y., Liu B., Tao X, & Li Z. (2023). Sorafenib/2800Z Co-Loaded into Cholesterol and PEG Grafted Polylysine NPs for Liver Cancer Treatment. Pharmaceuticals, 16(1), 119.

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In Vivo Imaging of U87 Tumor Growth

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In vivo imaging experiments using U87 in which iRFP720 was expressed were performed with 3 mice in each group. Images were obtained twice a week using IVIS Lumina LT in vivo imaging system (PerkinElmer, Inc., Waltham, MA, USA). To confirm the tumor growth tendency, Living Image software (ver.4.5) was used.

Maeyama M., Tanaka K., Nishihara M., Irino Y., Shinohara M., Nagashima H., Tanaka H., Nakamizo S., Hashiguchi M., Fujita Y., Kohta M., Kohmura E, & Sasayama T. (2021). Metabolic changes and anti-tumor effects of a ketogenic diet combined with anti-angiogenic therapy in a glioblastoma mouse model. Scientific Reports, 11, 79.

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Measuring Bacterial Pathogenicity In Vivo

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The regulatory regions of ipf and klf were cloned into pCS26 upstream to a promoterless luxCDABE operon (pCS26::Pipf and pCS26::Pklf, respectively) and transformed into S. Infantis 119944. The reporter strains were grown for 16 h in LB supplemented with kanamycin at 37°C and ~8×10⁸ CFU were used to infect by oral gavage female C57BL/6 mice (Envigo, Israel) that were pretreated with streptomycin. Similarly, one day old SPF White Leghorns chicks (Charles River) were infected orally (inter crop) with ~1×10⁷ CFU of the reporter strains in 0.2 ml saline. At 24h p.i., mice and chicks were sacrificed and their intact gastrointestinal tracts were removed and imaged using a photon-counting in-vivo imaging system (Photon-Imager, Biospace Lab or IVIS Lumina LT, PerkinElmer). To determine bacterial loads, the organs were homogenized in 0.7 ml saline using a BeadBlaster 24 homogenizer (Benchmark Scientific), serially diluted and plated on XLD agar plates supplemented with kanamycin.

Aviv G., Elpers L., Mikhlin S., Cohen H., Vitman Zilber S., Grassl G.A., Rahav G., Hensel M, & Gal-Mor O. (2017). The plasmid-encoded Ipf and Klf fimbriae display different expression and varying roles in the virulence of Salmonella enterica serovar Infantis in mouse vs. avian hosts. PLoS Pathogens, 13(8), e1006559.

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Assessing TOR Silencing Effects on Viral Spread

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To test whether TOR silencing can delay systemic infection of plant viruses, A. tumefaciens harbouring a TMV‐GFP clone was infiltrated into the fourth leaf of 4‐week‐old TOR‐silenced and control N. benthamiana plants as previously described by Hak and Spiegelman (2020 (link)). The clone was obtained from Dr Ziv Spiegelman, ARO. Seven days after infection, GFP fluorescence was measured using in vivo imaging analysis (IVIS Lumina LT, Perkin Elmer) equipped with a XFOV‐24 lens and Living Image 4.3.1 software (Perkin Elmer) set (excitation/emission: 420/520 nm). The data from the optical luminescence image were displayed in pseudocolour representing intensity terms of radiance (photons⋅s⁻¹⋅cm⁻²⋅steradian⁻¹) and calculated as average radiance per leaf.

Marash I., Leibman‐Markus M., Gupta R., Avni A, & Bar M. (2022). TOR inhibition primes immunity and pathogen resistance in tomato in a salicylic acid‐dependent manner. Molecular Plant Pathology, 23(7), 1035-1047.

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