Mice were anesthetized with 2% isofluorane in O2 and ventricular systolic pressure was assessed by catheterization using a Millar SPR-869 pressure-volume catheter.
Spr 869 pressure volume catheter
Manufactured by Millar
The SPR-869 pressure-volume catheter is a medical device designed to measure intravascular pressure and volume. It functions by using a pressure sensor and volume measurement capabilities to provide real-time data on these physiological parameters.
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4 protocols using spr 869 pressure volume catheter
1
Ventricular Pressure Assessment in Mice
2
Evaluation of BMP9 in Pulmonary Hypertension
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Male Sprague Dawley rats (~200 to 250 grams, Charles River, Margate, UK) were randomized into three groups. Control rats were given intraperitoneal injections of sterile water as a vehicle control. Rats on the prevention protocol were given 60 mg/kg of MCT (Sigma, St. Louis, MO) by i.p. injection and treated with daily i.p. injections of recombinant human BMP9 (600 ng/day) or vehicle control from day 0 until sacrifice on days 2, 5 or 21. Rats on the reversal protocol were given 40 mg/kg of MCT, allowed to develop PAH from days 0 to 21, and treated with BMP9 (600 ng/day) or vehicle control from day 21 to sacrifice on day 35. At the time of sacrifice, animals were anaesthetized with xylazine (4.6 mg/kg) and ketamine (7 mg/kg) and right and left ventricular function were assessed using a Millar SPR-869 pressure-volume catheter. Tissue harvesting was carried out as described above for mice.
3
Sugen-Induced Pulmonary Hypertension Model
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Male Sprague Dawley rats (∼150–200 g, Charles River, Saffron Walden, Essex, UK) were administered a single subcutaneous injection of Sugen 5416 (SU-5416; 20 mg kg−1, Tocris, Bristol, UK) in vehicle (0.5% carboxyl methylcellulose sodium, 0.4% polysorbate 80, 0.9% benzyl alcohol, all Sigma-Aldrich). Subsequently, rats were placed into a 10% O2 chamber for 3 weeks. After 3 weeks of hypoxia, animals were returned to a normoxic environment for 5 weeks. At 8 weeks, rats were randomized into two groups. One group received i.p. injections of 2.5 mg kg−1 Etanercept (Enbrel Pfizer, Sandwich, Kent, UK) diluted in Dulbecco's phosphate-buffered saline (D8537, Sigma-Aldrich) and the second group received vehicle alone. For terminal hemodynamic measurements, rats were anesthetized with xylazine (4.6 mg kg−1) and ketamine (7 mg kg−1). Body weight was recorded. Right and left ventricular function were assessed using a Millar SPR-869 pressure-volume catheter. Tissue collecting and assessment of right ventricular hypertrophy were conducted as described above for mice.
4
Evaluation of BMP9 in Pulmonary Hypertension
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