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4 protocols using concentrated hydrochloric acid hcl

1

Synthesis and Characterization of Glucosamine Derivatives

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D(+)-Glucosamine hydrochloride, D-Glucosaminic, and D-Glucosamine sulphate were from Aladdin Co., Ltd., Shanghai, China. 2-Acetamido-2-deoxy-L-glucopyranose, N-Methyl-D-glucamine, and Zinc chloride were purchases from MACKLIN Co., Ltd., Shanghai, China. Concentrated hydrochloric acid (HCl, 99.0%) was obtained from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China.
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2

Synthesis of Acetylacetone Cobalt(II) Catalyst

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All chemical regents including concentrated hydrochloric acid (HCl), polyvinyl alcohol, ethyl alcohol and acetylacetone cobalt(II)(AACo) were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Olive oil (Olivoilà) and deionized water obtained from a Milli-Q system (Millipore, Bedford, MA) were used.
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3

Chondrogenic Differentiation of Rat BMSCs

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Dopamine (DA) and tris-(hydroxymethyl)-aminomethane (Tris) were purchased from Sigma-Aldrich (USA). Concentrated hydrochloric acid (HCl) was obtained from Sinopharm Chemical Reagent Co., Ltd (China). Polymer PCL (molecular weight = 45 kDa) was purchased from Sigma-Aldrich (USA). Gelatin (Gel), hyaluronic acid (HA), sodium alginate (SA), and calcium chloride anhydrous (CaCl2) were purchased from Aladdin Co., Ltd. (China). CTGF (recombinant, rat) was purchased from R&D systems (USA) and TGF-β3 (recombinant, rat) was purchased from ProSpec (USA). Cell culture reagents, such as Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and trypsin were purchased from Life Technologies (USA). The Rat (SD) Mesenchymal Stem Cell Chondrogenic Differentiation Basal Medium was purchased from Cyagen Biosciences Inc. (China). BMSCs were extracted from the bone marrow of Sprague Dawley (SD) rats. SD rats and nude mice were obtained from the Animal Laboratory Center of Shanghai Jiao Tong University (China).
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4

Microencapsulation of Probiotic Cultures

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Man Rogosa Sharpe (MRS) broth was obtained from Haibo Biotechnology Co. Ltd. (Qingdao, China). Agar, sodium chloride (NaCl), and glycerol were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). For the microencapsulation experiments (including preparation and characterization), sodium alginate, Tween 80, glacial acetic acid, calcium carbonate, sodium L (+)-ascorbate, sodium citrate, sodium bicarbonate, potassium phosphate monobasic, and dipotassium hydrogen phosphate were purchased from Sinopharm Chemical Reagent Co. Ltd. Chitosan hydrochloride and trehalose were purchased from Aladdin Reagent Co. Ltd. (Shanghai, China). Skim milk powder was purchased from NZMP (Auckland, New Zealand). Bean oil was purchased from Yihai Kerry (Shanghai, China). For simulated digestion experiments, sodium hydroxide (NaOH) and concentrated hydrochloric acid (HCl) were purchased from Sinopharm Chemical Reagent Co. Ltd. Trypsin was purchased from Amresco (Dallas, TX, United States). Pepsin and bile salts were purchased from Sigma-Aldrich (St. Louis, MO, United States).
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