Rodent lab chow 5008

For all experiments with wild type (wt) mice, C57BL/6 mice were purchased from Jackson Laboratories. Experiments were performed primarily at VA Ann Arbor Healthcare System, with additional experiments at National Jewish Health; both Animal Care Facilities are fully accredited by the American Association for Accreditation of Laboratory Animal Care. Mice were housed under specific pathogen-free conditions and used for experiments between 8–16 weeks of age. To generate miR-34a+/− mice, miR-34a flox/flox mice on a C57BL/6 background (37 (link)) (Jackson) were crossed with LysM cre mice (Jackson). The F1 generation of miR-34a flox/− LysM cre mice (referred to as miR-34a+/−) was genotyped following Jackson protocols, with non-littermate, age-matched C57BL/6 mice as wt controls in all such experiments. Mice were fed standard animal chow (rodent lab chow 5008; Purina, St. Louis, MO) and chlorinated tap water ad libitum. Animal care and experimentation were conducted in accordance with U.S. Department of Health and Human Services Guide for the Care and Use of Laboratory Animals and were approved by the Animal Use Committee at VA Ann Arbor Healthcare System and National Jewish Health.

C57BL/6 mice were purchased from Jackson Laboratories (Bar Harbor, ME). Mice were housed under specific pathogen-free conditions in the Animal Care Facility at the VA Ann Arbor Healthcare System, which is fully accredited by the American Association for Accreditation of Laboratory Animal Care. Mice were fed standard animal chow (rodent lab chow 5008; Purina, St. Louis, MO) and chlorinated tap water ad libitum and were used for experiments between 8 and 16 weeks of age. Animal care and experimentation were conducted in accordance with the Guide for the Care and Use of Laboratory Animals (8th edition) and were approved by the Ann Arbor VA Healthsystem Subcommittee on Animal Studies.