Bicinchoninic acid bca proteinassay kit

Manufactured by Takara Bio

Sourced in Japan

The Bicinchoninic acid (BCA) protein assay kit is a colorimetric detection and quantification method for proteins. It provides a simple and accurate way to determine the total protein concentration in a sample.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Protease inhibitor tablet, by Thermo Fisher Scientific (1 mentions) Taurocholate, by Merck Group (1 mentions) Streptavidin agarose resin, by Thermo Fisher Scientific (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Estradiol 17β glucuronide, by Merck Group (1 mentions) Estrone 3 sulfate, by Merck Group (1 mentions) Sulfo n hydroxysuccinimide ss biotin, by Thermo Fisher Scientific (1 mentions) Bl21 de3, by Nippon Gene (1 mentions) Bradford protein assay kit, by Takara Bio (1 mentions)

Close spelling variants of this product

Bicinchoninic acid kit (4 citations) BCA kit (41 citations)

2 protocols using bicinchoninic acid bca proteinassay kit

Transporters and Membrane Protein Analysis

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Phenolsulfonphthalein (PSP)
was obtained from Macklin (Shanghai, China). Estradiol-17β-glucuronide
(E17βG), estrone-3-sulfate (E3S), and taurocholate (TCA) were
purchased from Sigma-Aldrich (St. Louis, MO). 4-(4-(Dimethylamino)styryl)-N-methylpyridinium (ASP⁺) and penciclovir (PCV)
were from US Everbright (Suzhou, China) and Aladdin (Shanghai, China),
respectively. Dulbecco’s modified Eagle’s medium (DMEM),
PSP-free DMEM, fetal bovine serum (FBS), trypsin, Lipofectamine 2000,
sulfo-N-hydroxysuccinimide-SS-biotin,
protease inhibitor tablets, and streptavidin-agarose resin were purchased
from Thermo Fisher Scientific (Waltham, MA). Penicillin-streptomycin
was from Hyclone (Logan, UT). The bicinchoninic acid (BCA) protein
assay kit was purchased from Takara (Kyoto, Japan). The first antibodies
for detecting 6-His tag and Na⁺/K⁺-ATPase were
from Proteintech (Rosemont, IL) and Abcam (Boston, MA), respectively.
Their secondary antibodies were from Proteintech (Rosemont, IL).

Wang Z., Li Y., Peng T., Su Y., Luo X., Han W., Zhang H., Ruan J, & Gui C. (2021). Human Organic Anion Transporting Polypeptides 1B1, 1B3, and 2B1 Are Involved in the Hepatic Uptake of Phenolsulfonphthalein. ACS Omega, 6(51), 35844-35851.

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Bacterial Cloning and Recombinant Protein Expression

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E. coli strain DH5α and BL21(DE3) were purchased from Nippon Gene (Tokyo, Japan) and were used for cloning and recombinant protein expression, respectively. A cloning vector, pTac-1, was purchased from BioDynamics (Tokyo, Japan). A modified pCold vector (TaKaRa, Shiga, Japan)^{47 (link)} was used for protein expression. Reagents were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan) unless stated otherwise. Protein concentration was determined with a TaKaRa bicinchoninic acid (BCA) protein assay kit or TaKaRa Bradford protein assay kit.

Nishiyama R., Ojima T., Ohnishi Y., Kumaki Y., Aizawa T, & Inoue A. (2021). An oxidative metabolic pathway of 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEHU) from alginate in an alginate-assimilating bacterium. Communications Biology, 4, 1254.

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