Ugt1a10

Manufactured by Corning

UGT1A10 is a laboratory equipment product from Corning. It is a recombinant enzyme used for in vitro studies. The core function of UGT1A10 is to catalyze the conjugation of various substrates with glucuronic acid.

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2 protocols using ugt1a10

In Vitro Licochalcone A Metabolism

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Licochalcone A (E-97%, Z-3% by NMR, Electronic Supplemental Material S1) and β-nicotinamide adenine dinucleotide phosphate, reduced sodium salt (NADPH), uridine 5’-diphosphoglucuronic acid triammonium salt (UDPGA), sulfatase, β-glucuronidase, sulfur trioxide-pyridine complex, and glutathione (GSH) were purchased from Sigma-Aldrich (St. Louis, MO). Pooled human liver microsomes (20 mg/mL, 150 men and women donors), cDNA-expressed human cytochrome P450 1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C18, CYP2C19, CYP2D6, CYP2E1, and CYP3A4 (0.5 nmol of P450 in 0.5 mL) and cDNA-expressed recombinant human UGTs including UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A7, UGT1A8, UGT1A9, UGT1A10, UGT2B4, UGT2B7, UGT2B15, and UGT2B17 (5 mg protein/mL), were purchased from Corning Life Sciences (Tewksbury, MA). HPLC-grade solvents were purchased from Thermo Fisher (Pittsburgh, PA). Cryopreserved human hepatocytes (50 donors pooled) and manufacturer-specified cell culture media were purchased from Thermo Fisher (Waltham, MA).

Huang L., Nikolic D, & van Breemen R.B. (2017). Hepatic metabolism of licochalcone A, a potential chemopreventive chalcone from licorice (Glycyrrhiza inflata), determined using liquid chromatography-tandem mass spectrometry. Analytical and bioanalytical chemistry, 409(30), 6937-6948.

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Comprehensive In Vitro ADME Toolkit

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Corning UltraPool human liver microsomes (HLM) 150 (20 mg/mL), human intestinal microsomes (HIM) pool (10 mg/mL) as well as recombinant human CYP enzymes (with P450 oxidoreductase, OR) including CYP1A2+OR (0.5 nmol), CYP2B6+OR (0.5 nmol), CYP2C8+OR (1.0 nmol), CYP2C9*1+OR (1 nmol), CYP2C19+OR (0.5 nmol), CYP2D6*1+OR (0.5 nmol), CYP2E1+OR + cytochrome b₅ (1 nmol), CYP3A4+OR + cytochrome b₅ (0.5 nmol), MAO-A (5 mg/mL), MAO-B (5 mg/mL), and UGT1A10 (5 mg/mL) were purchased from Corning Life Sciences B.V (Amsterdam, Netherlands). All microsomes and recombinant enzyme solutions were aliquoted and stored at −80 °C until further use. Co-factors including NADPH regenerating system solution A (25 mM NADP+, 66 mM glucose-6-phosphate, and 66 mM MgCl₂ in water), NADPH regenerating system solution B (40 U/mL glucose-6-phosphate dehydrogenase in 5 mM sodium citrate), UGT reaction mix solution A (25 mM uridine 5′-diphospho-glucuronic acid in water), and UGT reaction mix solution B (250 mM Tris-HCl, 40 mM MgCl₂, and 0.125 mg/mL alamethicin in water) were also obtained from Corning Life Sciences B.V.

Thomann J., Kolaczynska K.E., Stoeckmann O.V., Rudin D., Vizeli P., Hoener M.C., Pryce C.R., Vollenweider F.X., Liechti M.E, & Duthaler U. (2024). In vitro and in vivo metabolism of psilocybin’s active metabolite psilocin. Frontiers in Pharmacology, 15, 1391689.

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