Anti cd5fitc

Manufactured by BioLegend

Sourced in China

Anti-CD5 FITC is a fluorescently labeled monoclonal antibody that recognizes the CD5 antigen. CD5 is a pan-T cell marker expressed on the surface of T cells and a subset of B cells. The FITC (Fluorescein Isothiocyanate) fluorochrome conjugated to the antibody allows for the detection and identification of CD5-positive cells using flow cytometry.

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2 protocols using anti cd5fitc

Thymus Cell Suspension Preparation and Staining

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Thymus cell suspensions were prepared and stained as described (Hager-Theodorides et al., 2005 (link)) using combinations of the following directly conjugated antibodies at concentration of 1:100: (from BD Pharmingen) anti-γδPE (catalogue no. 553178); from eBioscience: anti-TCRβFITC (catalogue no. 11-5961-85), antiCD3PE (catalogue no. 12-0031-82), anti-CD24PE (catalogue no. 12-0241-82) and anti-CD69FITC (catalogue no. 11-0691-85); (from Biolegend) anti-CD3FITC (catalogue no. 100204), anti-CD5FITC (catalogue no. 100605), anti-Qa2FITC (catalogue no. 121709), anti-CD4APC (catalogue no. 116014), anti-CD5PE (catalogue no. 100607), anti-CD8PerCP/Cy5.5 (catalogue no. 100734), anti-CD4PerCP/Cy5.5 (catalogue no. 100539) and anti-CD8APC (catalogue no. 100712). Data were acquired on a C6 Accuri flow cytometer (BD Biosciences) and analysed using FlowJo software. Live cells were gated by FSC and SSC profiles. Data represent at least three experiments.

Solanki A., Yanez D.C., Ross S., Lau C.I., Papaioannou E., Li J., Saldaña J.I, & Crompton T. (2018). Gli3 in fetal thymic epithelial cells promotes thymocyte positive selection and differentiation by repression of Shh. Development (Cambridge, England), 145(3), dev146910.

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Comprehensive Identification of ILC Subsets

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Peripheral blood mononuclear cells were stained using the following antibodies: anti-CD3-FITC (clone: HIT3a), anti-CD5-FITC (clone: UCHT2), anti-CD11c-FITC (clone: 3.9), anti-CD16-FITC (clone: B73.1), anti-CD19-FITC (clone: HIB19), anti-TCRαβ-FITC (clone: IP26), anti-CD117-PE (clone: A3C6E2), anti-CD127-PE/Cyanine7 (clone: A019D5), anti-CD294-APC (clone: BM16), and anti-CD45-APC/Cyanine7 (clone: H130) (all from BioLegend, Beijing, China). Dead cells were stained with 7-aminoactinomycin D (7-AAD) viability staining solution (BioLegend). Total ILCs were identified as 7-AAD -CD45 + lineage (CD3, CD5, CD11c, CD16, CD19, and TCRαβ) -CD127 + lymphocytes. The ILC1s were CD117 -CD294 -, whilst ILC2s were CD294 + , and ILC3s were CD117 + CD294 -. Flow cytometry was performed using a CytoFLEX flow cytometer (Beckman Coulter, Brea, USA), and data were analyzed using CytoExpert v. 2.3 software (Beckman Coulter).

Chen Z.X., Liu H.Q., Wu Z.H., He J.L, & Zhong H.J. (2023). Type 3 innate lymphoid cells as an indicator of renal dysfunction and serum uric acid in hyperuricemia. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 32(3).

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