The drugs used were the following: AM404 [R&D Systems (Minneapolis, MN) and Fisher Scientific (Waltham, MA)]; MRS1477 and AM251 [Fisher Scientific]; capsazepine [VWR (Radnor, PA) and Cayman Chemical (Ann Arbor, MI)]; CP-55,940 [Santa Cruz Biotechnology (Dallas, TX)]; JZL184 [Sigma-Aldrich (St. Louis, MO) and Cayman Chemical]. All drugs were dissolved in 5% DMSO, 15% Tween 80 in sterile physiological saline and injected intraperitoneal at 10 mL/kg. The doses of drugs used were based on previous studies: AM404 at 10 mg/kg (Gamaleddin et al., 2013 (link)), MRS1477 at 10 mg/kg (Kaszas et al., 2012 (link)), AM251 at 1 mg/kg (Chen et al., 2004 (link)), capsazepine at 5 mg/kg (dos-Santos-Pereira et al., 2016 (link)), CP-55,940 at 30 μg/kg (Vinod et al., 2008 (link)), and JZL184 at two doses: 2 mg/kg and 18 mg/kg (Oleson et al., 2012 (link); Hartley et al., 2016 (link)).
Cp 55 940
Manufactured by Santa Cruz Biotechnology
Sourced in United States
CP-55,940 is a synthetic cannabinoid compound that functions as a potent agonist of the CB1 and CB2 cannabinoid receptors. It has been used extensively in research to study the pharmacological effects of cannabinoid receptor activation.
Automatically generated - may contain errors
Lab products found in correlation
Am404, by R&D Systems (1 mentions)
Am251, by Thermo Fisher Scientific (1 mentions)
Jzl184, by Merck Group (1 mentions)
Endoxifen, by Merck Group (1 mentions)
Lasofoxifene, by Merck Group (1 mentions)
Toremifene, by Merck Group (1 mentions)
Tamoxifen, by Bio-Techne (1 mentions)
Win55 212 2, by Bio-Techne (1 mentions)
Am251, by Bio-Techne (1 mentions)
Am630, by Bio-Techne (1 mentions)
N desmethyltamoxifen, by Merck Group (1 mentions)
35s gtpγs, by American Radiolabeled Chemicals (1 mentions)
Damgo, by Bio-Techne (1 mentions)
4 hydroxytamoxifen, by Merck Group (1 mentions)
2 protocols using cp 55 940
1
Pharmacological Modulation of Cannabinoid Receptors
2
Ligand Binding and Functional Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All SERMs were obtained from the following commercial sources. tamoxifen and Y-134 were purchased from Tocris Bioscience (Bristol, United Kingdom). N-desmethyl tamoxifen, 4-hydroxy tamoxifen, endoxifen, SO4-tamoxifen, toremifene, 4-hydroxy toremifene, OSP, RAL, lasofoxifene, NAF, and BAZ were all obtained from Sigma Aldrich (St. Louis, MO, USA). Acolbifene was procured from AdooQ Bioscience (Irvine, CA, USA).
AM-630, AM-251, DAMGO, and WIN-55,212-2 were purchased from Tocris Bioscience. CP-55,940 was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). [35S]GTPγS (1250 Ci/mmol) was procured from American Radiolabeled Chemicals (St. Louis, MO, USA) and [3H]CP-55,940 (131.4 Ci/mmol) was purchased from PerkinElmer (Waltham, MA, USA).
All other reagents were purchased from Fisher Scientific Inc. (Pittsburgh, PA, USA). All compounds were dissolved in 100% DMSO to produce a stock concentration of 10 mM.
AM-630, AM-251, DAMGO, and WIN-55,212-2 were purchased from Tocris Bioscience. CP-55,940 was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). [35S]GTPγS (1250 Ci/mmol) was procured from American Radiolabeled Chemicals (St. Louis, MO, USA) and [3H]CP-55,940 (131.4 Ci/mmol) was purchased from PerkinElmer (Waltham, MA, USA).
All other reagents were purchased from Fisher Scientific Inc. (Pittsburgh, PA, USA). All compounds were dissolved in 100% DMSO to produce a stock concentration of 10 mM.
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