Kasumi 1

Human AML cell lines U937, THP‐1, Kasumi‐1, SKNO‐1, and ME‐1 were purchased from Cell Bank of Shanghai Institute of Biochemistry and Cell Biology. Primary leukemia cells from AML patients (The Affiliated DrumTower Hospital of Nanjing University Medical School, Nanjing, China) were collected using lymphocyte‐monocyte separation medium (Jingmei, Shanghai, China). Primary AML cells and AML cell lines were cultured in RPMI‐1640 medium, supplemented with 10% FBS, 100 U/mL of benzyl penicillin, and 100 U/mL of streptomycin in a humidified environment with 5% CO₂ at 37°C. All cells used were passaged in our laboratory for less than 3 months after resurrection.

Human chronic myelogenous leukemia K562 cells, human promyelocytic leukemia HL-60 cells, human acute T lymphocytic leukemia Jurkat cells, human acute myeloid leukemia cell line Kasumi-1, human cervical carcinoma HeLa cells, human cervical squamous carcinoma SiHa cells, human cervical cancer cell lines CaSki, human hepatocellular carcinoma BEL-7402, human lung adenocarcinoma cell line A549, human breast cancer MDA-MB-231, human prostate cancer PC-3, mouse hepatoma cell line H22, and murine breast carcinoma cell line 4T1 were obtained from Shanghai Cell Bank, Chinese Academy of Science. The human non-small cell lung cancer cell line NCI-H1975 was provided by the American Type Culture Collection. A549 cell line was cultured in Ham’s F12K medium (F12K) with 10% fetal bovine serum and 1% penicillin/streptomycin. The K562 cell line was cultured in Iscove’s Modified Dulbecco’s medium (IMDM) with 10% fetal bovine serum and 1% penicillin/streptomycin. HeLa cell line was cultured in modified Eagle’s medium (MEM) with 10% fetal bovine serum and 1% penicillin/streptomycin, and others were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium with 10% fetal bovine serum and 1% penicillin/streptomycin. All the cells were cultured at 37 °C with 5% CO₂ in a humidified incubator.