Clspa
The CLSPA is a compact and versatile laboratory instrument designed for spectroscopic analysis. It utilizes a collimated light source and a sensitive photodetector to accurately measure the absorption or transmission spectra of liquid samples across a wide range of wavelengths.
Lab products found in correlation
10 protocols using clspa
Isolation of Adipocytes from Bone Marrow and Subcutaneous Fat
Isolation of Primary Salivary Gland Epithelial Cells
male Sprague–Dawley rat submaxillary glands. Glands were removed
aseptically; the outer connective tissue was excised followed by washing
in Hank’s balanced salt solution (HBSS) supplemented with 1×
antibiotic/antimycotic (Life Technologies 100× antibiotic–antimycotic
solution, Grand Island, NY). Glands were cut into cubes of approximately
1 mm3 followed by washing 2× in HBSS using gravity
settling. Cubes were then suspended in HBSS supplemented with collagenase
(CLSPA, 150 units/mL) and hyaluronidase (150 units/mL) (Worthington
Biochemical Corporation, Lakewood, NJ) and dissociated for 45 min
at 37 °C with gentle rocking. The digests were passed through
a 70 μm nylon mesh, and isolated cells were collected by centrifugation
at 1300 rpm for 5 min. Cell pellets were resuspended in advanced DMEM:F12
supplemented with 2.5% FBS, 2 mM Glutamax, 100 units/mL penicillin,
100 units/mL streptomycin, 0.25 μg/mL fungizone, and 10 ng/mL
epidermal growth factor (EGF). Cells were subcultured by trypsinization
and used at passage 3.
Isolation of Peripheral and Bone Marrow Mononuclear Cells
Bone marrow samples (cohort C) were obtained from the femur shaft of patients of varying age (Table
Isolation of PBMCs and ATMCs
Isolation of BMMCs from Healthy Individuals
Isolation of Bone Marrow Mononuclear Cells
Actin cytoskeleton dynamics in egg chambers
Isolation of Bone Marrow and Peripheral Blood Mononuclear Cells
For the isolation of bone marrow mononuclear cells (BMMCs), a fragment of substantia spongiosa osseum, which would otherwise be discarded was collected during routine hip replacement surgery. The bone was further fragmented and treated with purified collagenase (CLSPA, Worthington Biochemical; 20 U/ml) in complete RPMI medium (RPMI 1640; Corning supplemented with 10% FCS, 100 U/ml penicillin, and 100 μg/ml streptomycin; Sigma) for 1 h at 37 °C. BMMCs were extracted using a filtered tube centrifugation step, and then purified using density gradient centrifugation (Lymphoprep®; Stemcell technologies). Heparinised blood from the same donors was collected, and peripheral blood mononuclear cells (PBMCs) were also purified by density gradient centrifugation.
Isolation of BM and PB Mononuclear Cells
Isolation of Bone Marrow and Peripheral Blood Mononuclear Cells
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!