The chambers were mounted on a fluorescence microscope (Nikon, Eclipse TE-2000U) with a 60× objective (Nikon, Plan Apo VC 60× (NA=1.40)). Images were recorded in two ways depending on the temporal and spatial requirements. The first method used an EM-CCD TV camera (Hitachi Kokusai Electric, KP-E500) attached to the side-port of the microscope while the second method used a conventional digital camera (Canon, EOS Kiss X7i) attached to the front port of the microscope. TV images were recorded on a PC by an image grabber board (The Imaging Source, DFG/SVI PCI grabber) and controller (The Imaging Source, IC Capture) in an uncompressed AVI format (UYVY, 640×480 pixels, 30 fps). Still images were recorded in TIFF format (0.5 sec exposure, ISO=12800, 3456×3456 pixels). A wide-angle image was obtained by an All-in-one Fluorescence Microscope (Keyence, BZ-X700). Images were analyzed on a PC by Image-J software.
Eos kiss x7i
Manufactured by Canon
Sourced in Japan
The Canon EOS Kiss x7i is a digital single-lens reflex (DSLR) camera. It features a 24.2-megapixel APS-C CMOS sensor and DIGIC 6 image processor. The camera supports Full HD video recording and has a 9-point autofocus system.
Automatically generated - may contain errors
Lab products found in correlation
M165 fc, by Leica (2 mentions)
Plan apo vc 60, by Nikon (1 mentions)
Bz x700, by Keyence (1 mentions)
Eclipse te2000 u, by Nikon (1 mentions)
Bz x710, by Keyence (1 mentions)
Szx16, by Olympus (1 mentions)
Cellsens standard 1, by Olympus (1 mentions)
Lsm 5.0 image browser software, by Zeiss (1 mentions)
Cm dii, by Thermo Fisher Scientific (1 mentions)
Cm3050, by Leica (1 mentions)
Tissue tec oct compound, by Sakura Finetek (1 mentions)
Bx53 microscope, by Olympus (1 mentions)
6 protocols using eos kiss x7i
1
Fluorescence Microscope Imaging Protocol
2
Quantitative Tissue Imaging for Skin Regeneration
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Skin regeneration in living animals was monitored using digital camera systems (EOS Kiss x7i, Canon, Tokyo, Japan; DP70, Olympus) attached on dissecting microscopes (M165 FC, Leica Microsystems; SZX16, Olympus). Transmitted light and fluorescence images of tissue sections were acquired by a digital light microscope (BZ-X710, Keyence, Osaka, Japan) or a charge-coupled device (CCD) camera system (DP73, cellSens Standard 1.6, Olympus; DP22, cellSens Standard, Olympus) attached to a fluorescence microscope (BX50; Olympus). Microscopic images were analyzed by Photoshop 21.0.0 (Adobe Systems, San Jose, CA, USA) as well as with software for image acquisition systems. Bands on agarose gels in PCR were scanned and quantified by NIH ImageJ software (http://imagej.nih.gov/ij/ (accessed on 2 February 2020)). Figures were prepared using Photoshop. Image, brightness, contrast, and sharpness were adjusted according to the journal’s guidelines.
3
Time-lapse imaging of maize root responses
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Maize roots were treated with mock solution or 50 µM yucasin+10 µM Kyn for 1h before being positioned in glass capillaries as described above. Roots were then incubated in an inverted orientation and photographed under either dark or light conditions. To obtain images in the dark, very dim green light was used as a safe light. Time-lapse photographs were taken at 5min intervals with a single-lens reflex camera (EOS Kiss x7i, Canon) with a programmable shutter controller (model E6315, Etsumi) during a 24h period. The pictures were converted into a sequential movie using the Graphic Converter 9 Lemke Software & QuickTime 7 Pro (Apple).
4
Fluorescence Imaging of Transgenic Newts
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Transgenic newts were monitored under a fluorescence dissecting microscope (M165 FC) which was installed with filter sets specific to AmCyan (CFP; Exciter: 436/20 nm; Emitter: 480/40 nm; Leica Microsystems), YFP (YFP; Exciter: 500/20 nm; Emitter: 535/30 nm; Leica Microsystems) and mCherry (Exciter: XF1044, 575DF25; Emitter: XF3402, 645OM75; Opto Science, Tokyo, Japan). Images were taken by a digital camera system (EOS Kiss x7i; Canon, Tokyo, Japan) attached to the microscope. Eyeball sections were examined on a fluorescence microscope (BX50; Olympus, Tokyo, Japan) which was also installed with the same filter sets as well as standard filter sets for EGFP, Texas red and DAPI. Their images were taken by a charge-coupled device camera system (DP73; cellSens Standard 1.6; Olympus) attached to this microscope (BX50; Olympus) or by a confocal microscope system (LSM510; LSM 5.0 Image Browser software; Carl Zeiss, Germany). Images were analyzed with software for the image acquisition systems and in Photoshop CS5 Extended (Adobe Systems, San Jose, CA). Figures and panels were prepared using Photoshop CS5 Extended. Images, brightness, contrast and sharpness were adjusted according to the journal’s guidelines.
5
Embryonic Somatopleure Labeling with DiI
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CM-DiI (Molecular Probes) at 1 mg/mL in 100% EtOH was diluted to 0.5 mg/mL with 3 M sucrose solution. To label the somatopleure, DiI solution was injected between the ectoderm and somatopleure using a pulled glass capillary (GD-1, Narishige, Tokyo, Japan) by mouth pipetting. The embryos were incubated until the desired developmental stages and fixed using 4% paraformaldehyde in phosphate-buffered saline (PBS) at 4°C overnight. For cryosectioning, the fixed embryos were immersed in PBS containing 30% (w/v) sucrose, and then embedded in Tissue-Tec O.C.T. compound (Sakura Finetek, Tokyo, Japan). The sections (14 μm thick) were prepared with cryostat (CM 3050, Leica Biosystems Nussloch, Nußloch, Germany) and observed under a fluorescence microscope with a UPlanFL N 40× objective lens (NA 0.75) (OLYMPUS, Tokyo, Japan). Images were taken with a digital camera (EOS kiss X7i, Canon, Tokyo, Japan) and processed using Fiji (ImageJ; National Institutes of Health) software. The
6
Sudan Black B Staining of Lipids
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Deparaffinized sections were immersed in .5% Sudan Black B in 70% ethanol for 30 s. Thereafter, sections were briefly immersed several times in 70% ethanol. To remove the ethanol, sections were immersed in 40%, 30%, and 20% ethanol, and finally in distilled water. After that, the glycerin-enclosed sections were secured with a coverslip.
Bright-field images of Sudan Black-stained sections were acquired using a BX-53 microscope (Olympus) with a 40× objective, and regions of interest (ROIs) were visualized using a complementary metal-oxide semiconductor camera (EOS kiss X7i; Canon) via a 5× lens (PE × 5; Olympus). Captured images (145 × 220 μm) were transferred to a computer.
Bright-field images of Sudan Black-stained sections were acquired using a BX-53 microscope (Olympus) with a 40× objective, and regions of interest (ROIs) were visualized using a complementary metal-oxide semiconductor camera (EOS kiss X7i; Canon) via a 5× lens (PE × 5; Olympus). Captured images (145 × 220 μm) were transferred to a computer.
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